Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,805,159	T→C	G264G (GGA→GGG)	waaP ←	lipopolysaccharide core heptose (I) kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,805,159	0	T	C	100.0%	135.9 / NA	38	G264G (GGA→GGG)	waaP	lipopolysaccharide core heptose (I) kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

TTATATTTTTTATCTTGTACTTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATAT  >  NC_000913/3805029‑3805286                                                                                                                                   |                                                                                                                                ttATATTTTTTATCTTGTACTTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGt                                                                                                                        <  1:134453/140‑1 (MQ=255)         tttATCTTGTACTTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCg                                                                                                                >  1:700528/1‑140 (MQ=255)                tGTACTTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTa                                                                                                         >  2:536543/1‑140 (MQ=255)                 gTACTTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTaa                                                                                                        <  2:603867/140‑1 (MQ=255)                       ttttttCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCt                                                                                                  <  2:700528/140‑1 (MQ=255)                                          aTTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCAt                                                                               >  1:336405/1‑140 (MQ=255)                                            ttttAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGt                                                                             <  1:381466/140‑1 (MQ=255)                                            ttttAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGt                                                                             <  1:472923/140‑1 (MQ=255)                                              ttAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTc                                                                           <  1:884458/140‑1 (MQ=255)                                                aaTTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCaa                                                                         >  1:263741/1‑140 (MQ=255)                                                 aTTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAAt                                                                        <  1:631476/140‑1 (MQ=255)                                                             cAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCa                                                            >  1:434004/1‑140 (MQ=255)                                                              aaCCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCa                                                            <  2:723070/139‑1 (MQ=255)                                                              aaCCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCa                                                            >  1:723070/1‑139 (MQ=255)                                                               aCCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAag                                                          <  1:210332/140‑1 (MQ=255)                                                                    aTTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACa                                                     <  1:404517/140‑1 (MQ=255)                                                                          aaaataaaTAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATAttt                                               <  2:263741/140‑1 (MQ=255)                                                                                 ataGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATAttt                                               >  2:150779/1‑133 (MQ=255)                                                                                 ataGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATAttt                                               <  1:150779/133‑1 (MQ=255)                                                                                       ataataAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCAt                                  >  1:64769/1‑140 (MQ=255)                                                                                       ataataAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCAt                                  >  2:676608/1‑140 (MQ=255)                                                                                               gttagttCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAAt                          >  1:431678/1‑140 (MQ=255)                                                                                               gttagttCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTGTGCGTAAAGGCATCCCAAAAt                          >  2:896742/1‑140 (MQ=255)                                                                                                 tagttCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTC‑TTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATccc                               <  1:109506/132‑1 (MQ=255)                                                                                                 tagttCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTC‑TTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATccc                               >  2:109506/1‑132 (MQ=255)                                                                                                  agttCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATaaa                       <  1:536543/140‑1 (MQ=255)                                                                                                  agttCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATaaa                       <  1:676608/140‑1 (MQ=255)                                                                                                   gttCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAAc                      >  2:236711/1‑140 (MQ=255)                                                                                                    ttCCAGTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAAcc                     >  1:844986/1‑140 (MQ=255)                                                                                                         gTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATATGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCAt                >  1:477320/1‑140 (MQ=255)                                                                                                         gTACATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCAt                >  2:95359/1‑140 (MQ=255)                                                                                                            cATACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATaaa             <  2:434004/140‑1 (MQ=255)                                                                                                              tACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATc           >  2:66680/1‑140 (MQ=255)                                                                                                              tACTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATc           >  2:167986/1‑140 (MQ=255)                                                                                                               aCTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCg          >  1:490698/1‑140 (MQ=255)                                                                                                               aCTAATAAATATTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCg          <  1:49673/140‑1 (MQ=255)                                                                                                                       atatTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGatat  <  2:412084/140‑1 (MQ=255)                                                                                                                       atatTTTTATAACCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGatat  <  1:260174/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                TTATATTTTTTATCTTGTACTTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAGTACATACTAATAAATATTTTTATAATCCTTTGCGTTGTGTTCGCTCTTTAATACGCTCGGCCTTAACGCTTGCCATGTTCAATAAATTCTGTTCAAGAGACAATATTTTGCGTAAAGGCATCCCAAAATAAACCTTCATAAATCGCCAGATAT  >  NC_000913/3805029‑3805286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑