Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,887,873	T→C	V349A (GTG→GCG)	mnmE →	5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,887,873	0	T	C	100.0%	231.5 / NA	64	V349A (GTG→GCG)	mnmE	5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/34);  total (30/34)

ATGGCACCACAACAGACGCCGTGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGTGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTACAGG  >  NC_000913/3887738‑3888010                                                                                                                                        |                                                                                                                                          aTGGCACCACAACAGACGCCGTGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAAc                                                                                                                                       >  1:139020/1‑140 (MQ=255) aTGGCACCACAACAGACGCCGTGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAAc                                                                                                                                       >  1:694928/1‑140 (MQ=255) aTGGCACCACAACAGACGCCGTGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAAc                                                                                                                                       >  2:922521/1‑140 (MQ=255) aTGGCACCACAACAGACGCCGTGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAAc                                                                                                                                       >  2:844901/1‑140 (MQ=255)        cacaACAGACGCCGTGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGt                                                                                                                                    >  1:587597/1‑136 (MQ=255)        cacaACAGACGCCGTGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGt                                                                                                                                    <  2:587597/136‑1 (MQ=255)                      tGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGtctc                                                                                                                  <  2:949742/140‑1 (MQ=255)                      tGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGtctc                                                                                                                  <  1:592357/140‑1 (MQ=255)                      tGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGtctc                                                                                                                  >  1:949742/1‑140 (MQ=255)                      tGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGtctc                                                                                                                  <  2:660746/140‑1 (MQ=255)                           ccGGCAGAGATCTGGCCGGAATTTATTGTCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGc                                                                                                             <  1:190529/140‑1 (MQ=255)                           ccGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGc                                                                                                             <  1:322375/140‑1 (MQ=255)                              gCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAg                                                                                                          <  1:650778/140‑1 (MQ=255)                              gCAGAGATCTGACCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAg                                                                                                          >  1:376197/1‑140 (MQ=255)                               cAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAgg                                                                                                         <  2:636270/140‑1 (MQ=255)                                agagATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGa                                                                                                        <  1:227590/140‑1 (MQ=255)                                agagATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGa                                                                                                        <  1:165853/140‑1 (MQ=255)                                  agaTCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACt                                                                                                      >  1:640033/1‑140 (MQ=255)                                  agaTCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACt                                                                                                      >  1:131361/1‑140 (MQ=255)                                    aTCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGtctc                                                                                                                  <  1:44548/126‑1 (MQ=255)                                    aTCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGtctc                                                                                                                  >  2:44548/1‑126 (MQ=255)                                    aTCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGtct                                                                                                                   <  2:644315/125‑1 (MQ=255)                                    aTCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGtct                                                                                                                   >  1:644315/1‑125 (MQ=255)                                    aTCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTgg                                                                                                    >  2:35000/1‑140 (MQ=255)                                         gCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAg                                                                                               >  2:471407/1‑140 (MQ=255)                                                  tATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACg                                                                                      <  2:376197/140‑1 (MQ=255)                                                  tATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACg                                                                                      <  1:891836/140‑1 (MQ=255)                                                        ccGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTgg                                                                                                 <  2:714146/123‑2 (MQ=255)                                                        ccGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTgg                                                                                                 >  1:714146/1‑122 (MQ=255)                                                        ccGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGtgctgc                                                                                >  1:697646/1‑140 (MQ=255)                                                        ccGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGtgctgc                                                                                >  2:873383/1‑140 (MQ=255)                                                          gTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGt                                                                              >  2:353300/1‑140 (MQ=255)                                                          gTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAA‑TCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTa                                                                             >  2:589512/1‑140 (MQ=255)                                                           tCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTa                                                                             >  1:429730/1‑140 (MQ=255)                                                           tCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTa                                                                             >  2:807441/1‑140 (MQ=255)                                                            cTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTaa                                                                            <  2:694928/140‑1 (MQ=255)                                                            cTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTaa                                                                            <  1:353300/140‑1 (MQ=255)                                                            cTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTaa                                                                            >  2:584057/1‑140 (MQ=255)                                                                      aaCTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCaaa                                                                  <  2:429730/140‑1 (MQ=255)                                                                      aaCTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCaaa                                                                  <  2:368568/140‑1 (MQ=255)                                                                       aCTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAAc                                                                 >  1:216506/1‑140 (MQ=255)                                                                             gATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCa                                                           <  1:584057/140‑1 (MQ=255)                                                                                   cGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCt                                                     <  2:216506/140‑1 (MQ=255)                                                                                     tggtgCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCa                                                           >  2:229087/1‑132 (MQ=255)                                                                                     tggtgCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCa                                                           <  1:229087/132‑1 (MQ=255)                                                                                     tggtgCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCttt                                                   <  1:35000/140‑1 (MQ=255)                                                                                     tggtgCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCttt                                                   >  2:222693/1‑140 (MQ=255)                                                                                     tggtgCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCttt                                                   >  1:531041/1‑140 (MQ=255)                                                                                       gtgCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGa                                                 <  2:697646/140‑1 (MQ=255)                                                                                       gtgCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGa                                                 >  1:129559/1‑140 (MQ=255)                                                                                        tgCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGac                                                <  1:222693/140‑1 (MQ=255)                                                                                         gcgcAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGaca                                               <  2:139020/140‑1 (MQ=255)                                                                                         gcgcAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGaca                                               <  1:938530/140‑1 (MQ=255)                                                                                         gcgcAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGaca                                               <  1:43398/140‑1 (MQ=255)                                                                                            cAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCa                                            <  2:129559/140‑1 (MQ=255)                                                                                                  aGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATgg                                      >  2:51290/1‑140 (MQ=255)                                                                                                   gCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGa                                     <  1:522959/140‑1 (MQ=255)                                                                                                    ccGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGaca                                               <  1:774660/129‑1 (MQ=255)                                                                                                    ccGATATCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGaca                                               >  2:774660/1‑129 (MQ=255)                                                                                                       atatCACCGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAggc                                 >  2:600522/1‑140 (MQ=255)                                                                                                        tatCACCGGCGAAACGCTGGGAATGAGTGACGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAggcg                                <  2:688603/140‑1 (MQ=255)                                                                                                              cGGCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTcc                          >  1:679327/1‑140 (MQ=255)                                                                                                                gCGAAACGCTGGGAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGGCACCAACATGGAAGGCGGCTTCCTg                        >  1:711383/1‑140 (MQ=255)                                                                                                                           ggAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGCGCGtcgtcg             <  1:844901/140‑1 (MQ=255)                                                                                                                           ggAATGAGTGAAGCGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGCGCGtcgtcg             <  1:922521/140‑1 (MQ=255)                                                                                                                                 agtgaagcgaACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGcgcg                   <  1:33867/128‑1 (MQ=255)                                                                                                                                 agtgaagcgaACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGcgcg                   >  2:33867/1‑128 (MQ=255)                                                                                                                                   tgaagcgaaCGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTAc     <  1:51290/140‑1 (MQ=255)                                                                                                                                    gaagcgaaCGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTACa    <  1:873383/140‑1 (MQ=255)                                                                                                                                     aagcgaaCGGTCACGCGTTAA‑TCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTACAgg  <  1:589512/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                          ATGGCACCACAACAGACGCCGTGGATCCGGCAGAGATCTGGCCGGAATTTATTGCCCGTCTGCCAGCGAAACTGCCGATCACCGTGGTGCGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAGTGAAGTGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTACAGG  >  NC_000913/3887738‑3888010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑