| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,087,770 | T→C | F257L (TTT→CTT) | yiiG → | DUF3829 domain‑containing lipoprotein YiiG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,087,770 | 0 | T | C | 98.4% | 216.2 / ‑4.7 | 61 | F257L (TTT→CTT) | yiiG | DUF3829 domain‑containing lipoprotein YiiG |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (35/25); total (36/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAATTAATGATAAGCGTCAGCTTGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGTTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAGGCCAGTATCAACTCGAGGCTAAA > NC_000913/4087638‑4087901 | gAAATTAATGATAAGCGTCAGCTTGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGc > 1:117783/1‑140 (MQ=255) tAATGATAAGCGTCAGCTTGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAg > 2:190199/1‑140 (MQ=255) tGATAAGCGTCAGCTTGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGTgg > 1:712545/1‑140 (MQ=255) gATAAGCGTCAGCTTGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGc < 2:889492/140‑1 (MQ=255) aaGCGTCAGCTTGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAAt > 1:11079/1‑140 (MQ=255) cGTCAGCTTGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATgaa > 1:3784/1‑140 (MQ=255) tCAGCTTGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATgaaga > 1:206668/1‑140 (MQ=255) cAGCTTGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATgaagaa < 1:960766/140‑1 (MQ=255) tGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAgtgt < 2:11079/140‑1 (MQ=255) aaCTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGaa < 1:530681/140‑1 (MQ=255) aaCTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGaa < 1:564246/140‑1 (MQ=255) cTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACt > 1:427289/1‑140 (MQ=255) tGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATgaagaa > 2:3770/1‑127 (MQ=255) tGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATgaagaa < 1:3770/127‑1 (MQ=255) aaTATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAAc < 2:206668/140‑1 (MQ=255) aagaaagaGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGa < 2:722647/130‑1 (MQ=255) aagaaagaGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGa > 1:722647/1‑130 (MQ=255) aaagaGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGgcg < 2:3784/140‑1 (MQ=255) agagAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCa > 2:913499/1‑140 (MQ=255) aGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCa < 2:676338/140‑1 (MQ=255) aGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCa > 2:303930/1‑140 (MQ=255) aaaaCATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAaaga > 2:557003/1‑140 (MQ=255) aaCATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAaagaag < 2:427289/140‑1 (MQ=255) ccACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATa > 1:163116/1‑140 (MQ=255) ccACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGAAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATa > 1:413190/1‑140 (MQ=255) actactactCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGGAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATaaa > 2:862939/1‑140 (MQ=255) tactactCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAaag > 2:218381/1‑127 (MQ=255) tactactCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAaag < 1:218381/127‑1 (MQ=255) ctactCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAACGTGACTGAACTGGAAACGCTAGTGGCGAAGGCCAAAGAAGCGGATAAAggcg > 2:701703/1‑140 (MQ=255) ctactCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAggcg > 1:189808/1‑140 (MQ=255) ctctctGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGGTAAAGGCGGCa > 1:734692/1‑140 (MQ=255) ctctGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATg > 2:942303/1‑140 (MQ=255) tctGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGa > 1:899079/1‑140 (MQ=255) tGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAggc < 1:528876/131‑1 (MQ=255) tGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAggc > 2:528876/1‑131 (MQ=255) gCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGGAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAAttt > 1:703427/1‑140 (MQ=255) gCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCa > 1:308536/1‑133 (MQ=255) gCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCa < 2:308536/133‑1 (MQ=255) aGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGGAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTtct < 2:703427/140‑1 (MQ=255) aGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTtct < 2:17487/140‑1 (MQ=255) gTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTtctc > 1:768159/1‑140 (MQ=255) gTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTtctc > 1:69705/1‑140 (MQ=255) tCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCg > 1:744177/1‑140 (MQ=255) aTGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGtt > 1:381626/1‑140 (MQ=255) aTGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGtt > 2:806894/1‑140 (MQ=255) aGCGAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAAtt > 2:606025/1‑140 (MQ=255) gAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGGTAAAGGCGGCATGAATTTCTCGTTTATTAATTCgg < 2:734692/140‑1 (MQ=255) gAAACAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCgg < 2:189808/140‑1 (MQ=255) aaaCAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGc < 1:303930/140‑1 (MQ=255) aaaCAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGc < 2:163116/140‑1 (MQ=255) aaaCAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGc < 1:606025/140‑1 (MQ=255) aaaCAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGAAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGc < 2:413190/140‑1 (MQ=255) aCAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAg > 2:649829/1‑140 (MQ=255) cAAATTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAgg > 2:151266/1‑140 (MQ=255) aaaTTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAGGc < 2:69705/140‑1 (MQ=255) aaaTTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAGGc < 1:649829/140‑1 (MQ=255) aaaTTAATAACCTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAGGc < 2:381626/140‑1 (MQ=255) cTGATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAGGCCAGTATCAACt < 1:806894/140‑1 (MQ=255) gATATCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGACAGGCCAGTATCAACTCg > 2:437116/1‑140 (MQ=255) atatCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAGGCCAGTATCAACTCGa > 2:334954/1‑140 (MQ=255) atatCGCAAGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAGGCCAGTATCAACTCGa > 2:136726/1‑140 (MQ=255) cAAGATAAGTTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAGGCCAGTATCAACTCGAGGCTaa > 1:760832/1‑140 (MQ=255) aaGATAAGCTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAGGGCAGTATCAACTCGAGGCTaaa > 2:896218/1‑140 (MQ=255) | GAAATTAATGATAAGCGTCAGCTTGCGGAACTGAAAAATATTGAAGAAAGAGAAGGGAAAACATTCCACTACTACTCTCTGGCAGTCATGATTTCAGCGAAACAAATTAATAACCTGATATCGCAAGATAAGTTTGATGCAGAAGCGGCAATGAAGAAAGTGTCTGAACTGGAAACGCTAGTGGCGCAGGCCAAAGAAGCGGATAAAGGCGGCATGAATTTCTCGTTTATTAATTCGGCAGGCCAGTATCAACTCGAGGCTAAA > NC_000913/4087638‑4087901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |