Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,277,878	A→G	D137G (GAC→GGC)	soxR →	DNA‑binding transcriptional dual regulator SoxR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,277,878	0	A	G	100.0%	168.0 / NA	47	D137G (GAC→GGC)	soxR	DNA‑binding transcriptional dual regulator SoxR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/26);  total (21/26)

AACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCC  >  NC_000913/4277743‑4278013                                                                                                                                        |                                                                                                                                        aaCAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGc                                                                                                                                     <  1:797349/140‑1 (MQ=255)     gCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGGGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCt                                                                                                                                    >  2:501247/1‑137 (MQ=255)     gCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGGGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCt                                                                                                                                    <  1:501247/137‑1 (MQ=255)     gCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAg                                                                                                                                 <  2:504517/140‑1 (MQ=255)              ccAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGaagaag                                                                                                                        >  2:766300/1‑140 (MQ=255)                       agaagaGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGgcg                                                                                                               >  1:914062/1‑140 (MQ=255)                         aagaGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCa                                                                                                             <  2:53666/140‑1 (MQ=255)                          agagTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCAc                                                                                                            >  2:205083/1‑140 (MQ=255)                           gagTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACg                                                                                                           <  2:571073/140‑1 (MQ=255)                            agTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGc                                                                                                          <  2:38713/140‑1 (MQ=255)                            agTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGc                                                                                                          <  1:766300/140‑1 (MQ=255)                            agTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGc                                                                                                          <  2:34738/140‑1 (MQ=255)                             gTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCt                                                                                                         <  1:111705/140‑1 (MQ=255)                             gTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCt                                                                                                         <  1:817872/140‑1 (MQ=255)                             gTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCt                                                                                                         <  2:69241/140‑1 (MQ=255)                              ttGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCt                                                                                                         <  2:102414/139‑1 (MQ=255)                              ttGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCt                                                                                                         >  1:102414/1‑139 (MQ=255)                                 gATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCt                                                                                                     <  1:523453/140‑1 (MQ=255)                                        gcATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGAt                                                                                              >  1:724669/1‑140 (MQ=255)                                                 ccTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAg                                                                                                <  2:298824/129‑1 (MQ=255)                                                 ccTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAg                                                                                                >  1:298824/1‑129 (MQ=255)                                                    tAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaa                                                                                  >  1:965036/1‑140 (MQ=255)                                                    tAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaa                                                                                  <  1:205083/140‑1 (MQ=255)                                                     aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaaa                                                                                 >  2:834554/1‑140 (MQ=255)                                                     aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaaa                                                                                 >  2:587112/1‑140 (MQ=255)                                                      gTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                                <  2:914062/140‑1 (MQ=255)                                                      gTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                                >  1:46792/1‑140 (MQ=255)                                                               cGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGTCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAg                                                                       >  2:919700/1‑140 (MQ=255)                                                                  gACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGgc                                                                    <  2:724669/140‑1 (MQ=255)                                                                                aTGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgtttgttt                                                      >  1:147307/1‑140 (MQ=255)                                                                                aTGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgtttgttt                                                      >  2:6147/1‑140 (MQ=255)                                                                                  gTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTc                                                    <  1:666074/140‑1 (MQ=255)                                                                                     ttGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCgg                                                 >  1:826350/1‑140 (MQ=255)                                                                                     ttGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCgg                                                 >  1:728787/1‑140 (MQ=255)                                                                                          tgtgGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGATTGTTTTCCGgtcttt                                            >  1:212783/1‑140 (MQ=255)                                                                                             ggCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtc                                         >  1:339728/1‑140 (MQ=255)                                                                                             ggCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtc                                         >  2:784948/1‑140 (MQ=255)                                                                                              gCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtct                                        <  2:339728/140‑1 (MQ=255)                                                                                              gCTGCC‑TTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtctt                                       <  1:504684/140‑1 (MQ=255)                                                                                                          gcAGTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGATTGTTTTCCGGTCTTTGTCTTTCTCTCTATcc                            <  2:212783/140‑1 (MQ=255)                                                                                                             gTGATTGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGc                         <  1:834554/140‑1 (MQ=255)                                                                                                                 ttGCCCGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGt                     <  2:438265/140‑1 (MQ=255)                                                                                                                      cGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTg                       <  2:697593/133‑1 (MQ=255)                                                                                                                      cGTTGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTg                       >  1:697593/1‑133 (MQ=255)                                                                                                                        ttGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACagg              >  1:40974/1‑140 (MQ=255)                                                                                                                        ttGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACagg              >  1:780846/1‑140 (MQ=255)                                                                                                                         tGCGTAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACagga             <  2:965036/140‑1 (MQ=255)                                                                                                                             tAACCCGGGCGGCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGt         <  2:826350/140‑1 (MQ=255)                                                                                                                                    ggcggcCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTccccc  <  1:784948/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCC  >  NC_000913/4277743‑4278013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑