Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,432,592	A→G	R155R (CGT→CGC)	ytfF ←	inner membrane protein YtfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,432,592	0	A	G	100.0%	204.3 / NA	57	R155R (CGT→CGC)	ytfF	inner membrane protein YtfF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/29);  total (28/29)

TGACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAACGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTCGCGCTGGCTATAAAGCAGA  >  NC_000913/4432459‑4432727                                                                                                                                      |                                                                                                                                        tgACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGcc                                                                                                                                   <  1:600698/140‑1 (MQ=255)       gcgcCTGCGCTGTTGCCCACATCATCGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCa                                                                                                                             <  2:120131/140‑1 (MQ=255)          ccTGCGCTGTTGCCCACATCATCGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCaaaa                                                                                                                          >  2:903309/1‑140 (MQ=255)           cTGCGCTGTTGCCCACATCATCGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAAt                                                                                                                         >  1:370096/1‑140 (MQ=255)             gcgcTGTTGCCCACATCATCGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCg                                                                                                                       >  2:930715/1‑140 (MQ=255)             gcgcTGTTGCCCACATCATCGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCg                                                                                                                       >  1:590467/1‑140 (MQ=255)                        cacaTCATCGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTg                                                                                                            >  2:552668/1‑140 (MQ=255)                             catcGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGtt                                                                                                         <  1:746003/138‑1 (MQ=255)                             catcGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGtt                                                                                                         >  2:746003/1‑138 (MQ=255)                             catcGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTa                                                                                                       <  1:929901/140‑1 (MQ=255)                                cGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACt                                                                                                    >  2:214373/1‑140 (MQ=255)                                cGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACt                                                                                                    <  2:162392/140‑1 (MQ=255)                                cGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACt                                                                                                    >  2:39170/1‑140 (MQ=255)                                cGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACt                                                                                                    >  1:153872/1‑140 (MQ=255)                                  gATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCa                                                                                                  <  1:959861/140‑1 (MQ=255)                                    tGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGc                                                                                                <  1:903309/140‑1 (MQ=255)                                     gTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCa                                                                                               >  1:45667/1‑140 (MQ=255)                                     gTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCa                                                                                               <  2:590467/140‑1 (MQ=255)                                     gTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCa                                                                                               >  1:865566/1‑140 (MQ=255)                                        tGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCa                                                                                               <  2:901478/137‑1 (MQ=255)                                        tGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCa                                                                                               >  1:901478/1‑137 (MQ=255)                                        tGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGCATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAata                                                                                            >  2:925150/1‑140 (MQ=255)                                              gATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAata                                                                                            >  2:38444/1‑134 (MQ=255)                                              gATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAata                                                                                            >  2:31153/1‑134 (MQ=255)                                              gATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAata                                                                                            <  1:31153/134‑1 (MQ=255)                                              gATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAata                                                                                            <  1:38444/134‑1 (MQ=255)                                              gATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTcaca                                                                                      <  1:236756/140‑1 (MQ=255)                                              gATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTcaca                                                                                      >  2:236756/1‑140 (MQ=255)                                                         agccagcGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGcc                                                                           <  1:930715/140‑1 (MQ=255)                                                         agccagcGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGcc                                                                           <  2:45667/140‑1 (MQ=255)                                                         agccagcGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGcc                                                                           <  2:153872/140‑1 (MQ=255)                                                              gcGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGc                                                                      <  2:370096/140‑1 (MQ=255)                                                                     ttGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACa                                                               <  1:552668/140‑1 (MQ=255)                                                                         gcAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATc                                                           >  1:136217/1‑140 (MQ=255)                                                                                  aCCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTggg                                                  >  2:950163/1‑140 (MQ=255)                                                                                   ccACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTgggg                                                 <  1:594920/140‑1 (MQ=255)                                                                                    cACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGc                                                <  1:214373/140‑1 (MQ=255)                                                                                       gCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAg                                             >  2:269383/1‑140 (MQ=255)                                                                                       gCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAg                                             >  2:744840/1‑140 (MQ=255)                                                                                       gCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAg                                             >  2:667151/1‑140 (MQ=255)                                                                                                aCCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCa                                    <  1:744840/140‑1 (MQ=255)                                                                                                aCCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCa                                    <  2:865566/140‑1 (MQ=255)                                                                                                          cTAACGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTg                          >  1:246370/1‑140 (MQ=255)                                                                                                            aaCGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGtt                            <  1:157058/136‑1 (MQ=255)                                                                                                            aaCGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGtt                            >  2:157058/1‑136 (MQ=255)                                                                                                             aCGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCg                       >  1:914321/1‑140 (MQ=255)                                                                                                             aCGCCAGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCg                       <  1:950163/140‑1 (MQ=255)                                                                                                                  aGCACGATGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTcgcg                  <  1:110303/140‑1 (MQ=255)                                                                                                                   gCACGATGCCAGAGGAATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTCgcgc                 >  1:429998/1‑140 (MQ=255)                                                                                                                         tGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTcgcg                  <  2:660627/133‑1 (MQ=255)                                                                                                                         tGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTcgcg                  >  1:660627/1‑133 (MQ=255)                                                                                                                         tGCCAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTCGCGCTGGCta           >  2:553102/1‑140 (MQ=255)                                                                                                                            cAGAGGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTCGCGCTGGCTATaa        <  1:553102/140‑1 (MQ=255)                                                                                                                              gagGTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATCTTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTCGCGCTGGCTATAAAg      <  2:858996/140‑1 (MQ=255)                                                                                                                                 gTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTCGCGCTGGCTATAAAGcg    <  1:944743/139‑2 (MQ=255)                                                                                                                                 gTATAGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTCGCGCTGGCTATAAAGcg    >  2:944743/1‑138 (MQ=255)                                                                                                                                   ataGCGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTCGCGCTGGCTATAAAGCAGa  <  1:841857/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TGACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGTTTGTCGGGATTTTCCCGCAGCCAGCGGGCGTTGCGCAGGGCATACCACGCCCAGCAGACCACGGAAACTAACGCCAGCACGATGCCAGAGGTATAACGTGCCCAGTCAAAATCGGGGAGTCCGTGGTTTAACTCAGCAATATTCACACACGCCAGGCCGATGCCAATACAAATCAGTGCTGGGGCGAGTTTTCCCCACGCGAGTTTGCCGTCGCGCTGGCTATAAAGCAGA  >  NC_000913/4432459‑4432727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑