Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,436,571	A→G	intergenic (‑6/‑184)	cpdB ← / → cysQ	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase/3'(2'),5'‑bisphosphate nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,436,571	0	A	G	100.0%	324.6 / NA	89	intergenic (‑6/‑184)	cpdB/cysQ	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase/3'(2'),5'‑bisphosphate nucleotidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/49);  total (40/49)

TCTTTGTAATAATCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGACATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGT  >  NC_000913/4436435‑4436706                                                                                                                                         |                                                                                                                                        tCTTTGTAATAATCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCAt                                                                                                                                      <  1:300463/140‑1 (MQ=255) tCTTTGTAATAATCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCAt                                                                                                                                      <  2:100717/140‑1 (MQ=255)          taatCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTatc                                                                                                                             <  2:191535/140‑1 (MQ=255)           aatCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTatca                                                                                                                            >  1:24910/1‑140 (MQ=255)           aatCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTatca                                                                                                                            <  1:552722/140‑1 (MQ=255)             tCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCGGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTatcatc                                                                                                                          >  1:768621/1‑140 (MQ=255)             tCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCtttt                                                                                                                                >  2:90874/1‑134 (MQ=255)             tCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCtttt                                                                                                                                <  1:90874/134‑1 (MQ=255)             tCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTatcatc                                                                                                                          >  2:119061/1‑140 (MQ=255)               gAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCa                                                                                                                                       >  1:432659/1‑125 (MQ=255)                 aaTCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGa                                                                                                                      >  2:349527/1‑140 (MQ=255)                  aTCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCtttt                                                                                                                                <  1:530034/129‑1 (MQ=255)                  aTCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCtttt                                                                                                                                >  2:530034/1‑129 (MQ=255)                      atcatGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACg                                                                                                                 <  1:849432/140‑1 (MQ=255)                       tcatGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACga                                                                                                                >  2:389020/1‑140 (MQ=255)                            ttGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACgaaagaa                                                                                                           <  2:103941/140‑1 (MQ=255)                               cTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAg                                                                                                        >  2:518342/1‑140 (MQ=255)                               cTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAg                                                                                                        >  1:367687/1‑140 (MQ=255)                                tATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAgg                                                                                                       <  1:948839/140‑1 (MQ=255)                                  tGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGaa                                                                                                                     >  2:430073/1‑124 (MQ=255)                                  tGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGaa                                                                                                                     <  1:430073/124‑1 (MQ=255)                                    cAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGgaga                                                                                                   >  2:315955/1‑140 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACgaaagaa                                                                                                           >  1:664622/1‑129 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACgaaagaa                                                                                                           <  2:664622/129‑1 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATa                                                                                                >  1:957284/1‑140 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATa                                                                                                >  2:442525/1‑140 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATa                                                                                                <  2:459177/140‑1 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATa                                                                                                >  2:202063/1‑140 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATa                                                                                                <  2:168546/140‑1 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATa                                                                                                <  2:531266/140‑1 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATa                                                                                                >  2:865040/1‑140 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATa                                                                                                >  1:631553/1‑140 (MQ=255)                                       aTCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATa                                                                                                >  2:736418/1‑140 (MQ=255)                                         cAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACgaaaga                                                                                                            >  1:455576/1‑126 (MQ=255)                                         cAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACgaaaga                                                                                                            <  2:455576/126‑1 (MQ=255)                                         cAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATaaa                                                                                              >  1:472510/1‑140 (MQ=255)                                            tGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGt                                                                                           <  1:512359/140‑1 (MQ=255)                                            tGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGt                                                                                           <  1:820051/140‑1 (MQ=255)                                            tGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGt                                                                                           >  2:860561/1‑140 (MQ=255)                                            tGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGt                                                                                           >  1:129316/1‑140 (MQ=255)                                             ggTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTc                                                                                          <  1:202063/140‑1 (MQ=255)                                             ggTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTc                                                                                          <  2:24910/140‑1 (MQ=255)                                             ggTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTc                                                                                          <  1:617896/140‑1 (MQ=255)                                              gTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTct                                                                                         >  2:918635/1‑140 (MQ=255)                                               tttCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTctt                                                                                        <  1:313690/140‑1 (MQ=255)                                               tttCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTctt                                                                                        <  1:315955/140‑1 (MQ=255)                                               tttCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTctt                                                                                        <  2:957284/140‑1 (MQ=255)                                               tttCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTctt                                                                                        <  2:779835/140‑1 (MQ=255)                                               tttCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTctt                                                                                        <  1:88151/140‑1 (MQ=255)                                               tttCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTctt                                                                                        <  1:442525/140‑1 (MQ=255)                                                    aTGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAAc                                                                                             >  2:941861/1‑130 (MQ=255)                                                    aTGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAAc                                                                                             <  1:941861/130‑1 (MQ=255)                                                    aTGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTcttactta                                                                                   <  2:487389/140‑1 (MQ=255)                                                    aTGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTcttactta                                                                                   <  2:129316/140‑1 (MQ=255)                                                    aTGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTcttactta                                                                                   <  2:321157/140‑1 (MQ=255)                                                      gATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTtata                                                                                 <  1:865040/140‑1 (MQ=255)                                                             aGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGt                                                                          >  2:467721/1‑140 (MQ=255)                                                             aGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGt                                                                          >  2:475077/1‑140 (MQ=255)                                                                         gCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCcac                                                              >  2:289368/1‑140 (MQ=255)                                                                              aTTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAAttt                                                         >  1:967346/1‑140 (MQ=255)                                                                                  acacTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACg                                                     >  1:45037/1‑140 (MQ=255)                                                                                    acTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCt                                                   <  2:967346/140‑1 (MQ=255)                                                                                       ggcggcAATCAGCGTGGCCAGGAGCGTTGCGCTAGACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGAGAAGAATGCCACAATTTTACGCTTTg                                                >  2:872760/1‑140 (MQ=255)                                                                                       ggcggcAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTg                                                >  2:285842/1‑140 (MQ=255)                                                                                       ggcggcAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTg                                                >  2:116233/1‑140 (MQ=255)                                                                                       ggcggcAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTg                                                >  1:497222/1‑140 (MQ=255)                                                                                       ggcggcAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTg                                                >  2:675843/1‑140 (MQ=255)                                                                                               tCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAatgat                                        <  2:45037/140‑1 (MQ=255)                                                                                               tCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAatgat                                        <  2:497222/140‑1 (MQ=255)                                                                                                 aGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAAtgatga                                      <  2:631553/140‑1 (MQ=255)                                                                                                   cGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGaca                                    <  1:285842/140‑1 (MQ=255)                                                                                                   cGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGaca                                    <  1:467721/140‑1 (MQ=255)                                                                                                                 ttGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGgc                      >  1:874145/1‑140 (MQ=255)                                                                                                                  tGCGCTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGgcg                     <  1:736418/140‑1 (MQ=255)                                                                                                                     gcTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGcat                  <  2:874145/140‑1 (MQ=255)                                                                                                                     gcTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGcat                  <  1:389020/140‑1 (MQ=255)                                                                                                                      cTAAACTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGcatt                 >  2:160765/1‑140 (MQ=255)                                                                                                                         aaCTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGCAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGcattcat              <  2:380320/140‑1 (MQ=255)                                                                                                                         aaCTTAATCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGcattcat              <  1:119061/140‑1 (MQ=255)                                                                                                                              aaTCATCAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACg         >  1:879246/1‑140 (MQ=255)                                                                                                                               atcatcAGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGa        <  1:475077/140‑1 (MQ=255)                                                                                                                                tcatcaGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGAt       <  1:289368/140‑1 (MQ=255)                                                                                                                                tcatcaGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGAt       <  2:266187/140‑1 (MQ=255)                                                                                                                                   tcaGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGa        <  2:581895/136‑1 (MQ=255)                                                                                                                                   tcaGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGa        >  1:581895/1‑136 (MQ=255)                                                                                                                                     aGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGt  <  2:242287/140‑1 (MQ=255)                                                                                                                                     aGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGt  >  2:873205/1‑140 (MQ=255)                                                                                                                                     aGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGt  <  1:349527/140‑1 (MQ=255)                                                                                                                                     aGGGGCATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAAGGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGt  <  1:860561/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCTTTGTAATAATCGAAATCCATCATGTTGCTATGCAGATCAGTGGTTTCCATGATACGCAGATCGACCGTCGCTGCATTCACACTGGCGGCAATCAGCGTGGCCAGGAGCGTTGCGCTAAACTTAATCATCAGGGACATCCTTTTATCATCGGGAATACGAAAGAAAAGGGAGAATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGT  >  NC_000913/4436435‑4436706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑