Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,621,090	T→C	C70R (TGC→CGC)	deoD →	purine nucleoside phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,621,090	0	T	C	100.0%	189.3 / NA	52	C70R (TGC→CGC)	deoD	purine nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/24);  total (28/24)

GCGTGCGAAGTATATTGCTGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCTGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCA  >  NC_000913/4620954‑4621225                                                                                                                                         |                                                                                                                                        gcgtgcgAAGTATATTGCTGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCt                                                                                                                                      <  2:141799/140‑1 (MQ=255) gcgtgcgAAGTATATTGCTGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCt                                                                                                                                      <  1:842782/140‑1 (MQ=255)    tgcgAAGTATATTGCTGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGc                                                                                                                                       >  2:9859/1‑136 (MQ=255)        aaGTATATTGCTGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTa                                                                                                                               <  2:131735/140‑1 (MQ=255)        aaGTATATTGCTGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTa                                                                                                                               <  2:612987/140‑1 (MQ=255)          gTATATTGCTGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTaca                                                                                                                             <  1:90881/140‑1 (MQ=255)              atTGCTGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCaa                                                                                                                         >  2:513157/1‑140 (MQ=255)                   tGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTa                                                                                                                               <  1:572647/129‑1 (MQ=255)                   tGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTa                                                                                                                               >  2:572647/1‑129 (MQ=255)                     aaaCTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTa                                                                                                                               >  1:650540/1‑127 (MQ=255)                     aaaCTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTa                                                                                                                               <  2:650540/127‑1 (MQ=255)                     aaaCTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTg                                                                                                                  >  2:837741/1‑140 (MQ=255)                     aaaCTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTg                                                                                                                  >  2:569428/1‑140 (MQ=255)                                   gATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCgg                                                                                                    >  2:477559/1‑140 (MQ=255)                                      gCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGt                                                                                                 <  1:513157/140‑1 (MQ=255)                                      gCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGt                                                                                                 >  2:957787/1‑140 (MQ=255)                                      gCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGt                                                                                                 >  2:113339/1‑140 (MQ=255)                                       cccGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCAc                                                                                                             <  2:36248/127‑1 (MQ=255)                                       cccGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCAc                                                                                                             >  1:36248/1‑127 (MQ=255)                                           tgaagtgaaCAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTgaaga                                                                                            >  1:824073/1‑140 (MQ=255)                                            gaagtgaaCAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTgaagaa                                                                                           <  1:113339/140‑1 (MQ=255)                                            gaagtgaaCAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTgaagaa                                                                                           <  1:957787/140‑1 (MQ=255)                                                  aacaacGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTAt                                                                                     <  1:405802/140‑1 (MQ=255)                                                   acaacGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATc                                                                                    <  2:889524/140‑1 (MQ=255)                                                       cGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCcgc                                                                                 >  2:497479/1‑139 (MQ=255)                                                       cGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCcgc                                                                                 <  1:497479/139‑1 (MQ=255)                                                       cGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCACAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCcgc                                                                                 >  1:252982/1‑139 (MQ=255)                                                       cGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCACAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCcgc                                                                                 <  2:252982/139‑1 (MQ=255)                                                         ttCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTg                                                                              <  2:438043/140‑1 (MQ=255)                                                                 aTGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCtg                                                                      <  1:477559/140‑1 (MQ=255)                                                                          ttCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGt                                                             >  2:777212/1‑140 (MQ=255)                                                                            cACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTc                                                           >  2:489893/1‑140 (MQ=255)                                                                              ccGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTg                                                         >  1:355668/1‑140 (MQ=255)                                                                                ggTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGcc                                                       <  1:777212/140‑1 (MQ=255)                                                                                        cAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTaa                                               <  2:355668/140‑1 (MQ=255)                                                                                         aaaGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTaaa                                              >  1:49943/1‑140 (MQ=255)                                                                                           aGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAAc                                            >  2:462971/1‑140 (MQ=255)                                                                                            ggCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACt                                           >  1:775809/1‑140 (MQ=255)                                                                                            ggCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACt                                           >  1:816491/1‑140 (MQ=255)                                                                                            ggCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACt                                           >  1:117138/1‑140 (MQ=255)                                                                                               cGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTgcg                                        >  2:513347/1‑140 (MQ=255)                                                                                               cGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTgcg                                        >  2:590868/1‑140 (MQ=255)                                                                                                gCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTgcgc                                       >  1:108553/1‑140 (MQ=255)                                                                                                           gTAATGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTAt                            <  2:117138/140‑1 (MQ=255)                                                                                                             aaTGGGTCACGGTATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGTTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCg                          <  2:497155/140‑1 (MQ=255)                                                                                                                         tATGGGTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCt              >  2:617475/1‑140 (MQ=255)                                                                                                                             ggTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCAc          <  1:462971/140‑1 (MQ=255)                                                                                                                              gTATCCCGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCAcc         >  2:626055/1‑140 (MQ=255)                                                                                                                                 tcccgtcccgCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGAt      >  1:656087/1‑140 (MQ=255)                                                                                                                                 tcccgtcccgCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGAt      >  2:635010/1‑140 (MQ=255)                                                                                                                                 tcccgtcccgCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGAt      >  1:738684/1‑140 (MQ=255)                                                                                                                                  cccGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGAtt     >  1:861815/1‑140 (MQ=255)                                                                                                                                  cccGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGAtt     <  2:738684/140‑1 (MQ=255)                                                                                                                                  cccGTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTATGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGAtt     >  2:526926/1‑140 (MQ=255)                                                                                                                                     gTCCCGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTATGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCa  <  1:526926/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GCGTGCGAAGTATATTGCTGAAACTTTCCTTGAAGATGCCCGTGAAGTGAACAACGTTCGCGGTATGCTGGGCTTCACCGGTACTTACAAAGGCCGCAAAATTTCCGTAATGGGTCACGGTATGGGTATCCCGTCCTGCTCCATCTACACCAAAGAACTGATCACCGATTTCGGCGTGAAGAAAATTATCCGCGTGGGTTCCTGTGGCGCAGTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCA  >  NC_000913/4620954‑4621225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑