Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	511,849	T→C	V70A (GTC→GCC)	glsA →	glutaminase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	511,849	0	T	C	100.0%	120.9 / NA	34	V70A (GTC→GCC)	glsA	glutaminase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/19);  total (15/19)

CCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCA  >  NC_000913/511720‑511982                                                                                                                                  |                                                                                                                                      ccGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTa                                                                                                                             <  2:643801/140‑1 (MQ=255)      tACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCt                                                                                                                        <  1:449137/140‑1 (MQ=255)      tACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCt                                                                                                                        <  1:772711/140‑1 (MQ=255)               tttCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTaga                                                                                                               <  2:816568/140‑1 (MQ=255)                           gTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGccc                                                                                                   <  1:468879/140‑1 (MQ=255)                                aGGTCAACTGGTGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAgg                                                                                              <  1:313131/140‑1 (MQ=255)                                    cAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAgg                                                                                              >  1:615979/1‑136 (MQ=255)                                    cAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAgg                                                                                              <  2:615979/136‑1 (MQ=255)                                      aCTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTAc                                                                                        <  1:183604/140‑1 (MQ=255)                                      aCTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTAc                                                                                        >  1:275774/1‑140 (MQ=255)                                      aCTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTAc                                                                                        >  1:460858/1‑140 (MQ=255)                                      aCTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTAc                                                                                        >  1:540997/1‑140 (MQ=255)                                       cTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACa                                                                                       >  1:433917/1‑140 (MQ=255)                                            ggcAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACa                                                                                  >  2:731878/1‑140 (MQ=255)                                                                    tGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGAt                                                          >  2:483774/1‑140 (MQ=255)                                                                      gCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTg                                                        <  2:275774/140‑1 (MQ=255)                                                                       cAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGc                                                       >  2:716440/1‑140 (MQ=255)                                                                                taGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACt                                              <  1:731878/140‑1 (MQ=255)                                                                                     cGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGtt                                         >  1:62431/1‑140 (MQ=255)                                                                                       ggTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTAt                                       <  2:460858/140‑1 (MQ=255)                                                                                             aGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCtt                                 <  1:644954/140‑1 (MQ=255)                                                                                                    aCCGCTTTGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTg                          <  1:716440/140‑1 (MQ=255)                                                                                                         tttGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATgg                     >  2:322267/1‑140 (MQ=255)                                                                                                           tGCACTGGAATCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAAGTCCTTTATCGCCTTAAAGTTGCATggcg                   >  2:687080/1‑140 (MQ=255)                                                                                                                     tCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCt         >  1:258777/1‑140 (MQ=255)                                                                                                                     tCCATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCt         >  1:922211/1‑140 (MQ=255)                                                                                                                       cATCTCGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCttt       >  1:766043/1‑140 (MQ=255)                                                                                                                           tcGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGcc   <  2:258777/140‑1 (MQ=255)                                                                                                                           tcGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGcc   <  2:922211/140‑1 (MQ=255)                                                                                                                            cGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCttt       <  1:821927/135‑1 (MQ=255)                                                                                                                            cGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCttt       >  2:821927/1‑135 (MQ=255)                                                                                                                            cGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCa  <  2:540997/140‑1 (MQ=255)                                                                                                                            cGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCa  <  1:483774/140‑1 (MQ=255)                                                                                                                            cGAAAGCCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCa  <  1:322267/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      CCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTACCGCTTTGCACTGGAATCCATCTCGAAAGTCTGTACGTTAGCCCTTGCGTTAGAAGATGTCGGCCCGCAGGCGGTACAGGACAAAATTGGCGCTGACCCGACCGGATTGCCCTTTAACTCAGTTATCGCCTTAGAGTTGCATGGCGGCAAACCGCTTTCGCCA  >  NC_000913/511720‑511982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑