Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	36,533	A→G	V431A (GTC→GCC)	caiC ←	carnitine‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	36,533	0	A	G	100.0%	141.3 / NA	40	V431A (GTC→GCC)	caiC	carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/22);  total (18/22)

CGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGACATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCT  >  NC_000913/36396‑36661                                                                                                                                          |                                                                                                                                 cGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCa                                                                                                                                >  2:603607/1‑140 (MQ=255)           ttCACCTTCATTCAGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTcgccgc                                                                                                                      <  2:480663/140‑1 (MQ=255)             cACCTTCATTCAGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCa                                                                                                                    <  1:603607/140‑1 (MQ=255)               ccTTCATTCAGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACg                                                                                                                  <  1:519807/140‑1 (MQ=255)               ccTTCATTCAGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACg                                                                                                                  <  1:798712/140‑1 (MQ=255)                  tcattcaGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACg                                                                                                                  >  2:714089/1‑137 (MQ=255)                  tcattcaGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACg                                                                                                                  <  1:714089/137‑1 (MQ=255)                  tcattcaGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGttt                                                                                                               <  2:467031/140‑1 (MQ=255)                    attcaGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTaa                                                                                                             >  1:642063/1‑140 (MQ=255)                      tcaGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATc                                                                                                           >  2:541033/1‑140 (MQ=255)                               caAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGttt                                                                                                               <  2:76067/127‑1 (MQ=255)                               caAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGttt                                                                                                               >  1:76067/1‑127 (MQ=255)                               caAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAg                                                                                                  <  1:618092/140‑1 (MQ=255)                                       ttGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAg                                                                                                  >  1:126350/1‑132 (MQ=255)                                       ttGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAg                                                                                                  <  2:126350/132‑1 (MQ=255)                                          aTGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATcgac                                                                                       <  1:836523/140‑1 (MQ=255)                                          aTGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATcgac                                                                                       >  2:836523/1‑140 (MQ=255)                                                    cgcgAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATaaaaa                                                                             >  2:695595/1‑140 (MQ=255)                                                             aatcTTTAATACCCACAACCACGATGTCCTGAATTTCCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCctct                                                                    <  2:596089/140‑1 (MQ=255)                                                                   tAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTtcgtcg                                                              <  1:541033/140‑1 (MQ=255)                                                                     aTACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTcgcg                                                            <  2:642063/140‑1 (MQ=255)                                                                     aTACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTcgcg                                                            <  2:836214/140‑1 (MQ=255)                                                                     aTACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTcgcg                                                            <  2:868408/140‑1 (MQ=255)                                                                           acaACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGcggtatcc                                                      >  1:447820/1‑140 (MQ=255)                                                                           acaACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGcggtatcc                                                      >  1:405314/1‑140 (MQ=255)                                                                            caACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGcggtatccg                                                     >  1:916658/1‑140 (MQ=255)                                                                            caACCACGATGTCCTGAATTTGCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGcggtatccg                                                     >  2:698831/1‑140 (MQ=255)                                                                             aaCCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGcggtatccgg                                                    >  1:869344/1‑140 (MQ=255)                                                                                  cGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTtcg                                                                 <  2:457365/122‑1 (MQ=255)                                                                                  cGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTtcg                                                                 >  1:457365/1‑122 (MQ=255)                                                                                       tCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGt                                          >  1:107101/1‑140 (MQ=255)                                                                                         cTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGt                                          <  1:444452/138‑1 (MQ=255)                                                                                         cTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGATATCGCCGGt                                          >  2:444452/1‑138 (MQ=255)                                                                                             aTTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGTATGcag                                    <  2:916658/140‑1 (MQ=255)                                                                                                 tCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGTATGcagccag                                >  2:202192/1‑140 (MQ=255)                                                                                                     gTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGTATGCAGCCAGCCAt                            <  1:202192/140‑1 (MQ=255)                                                                                                                           aGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGt      <  1:666512/140‑1 (MQ=255)                                                                                                                           aGCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGt      >  2:224789/1‑140 (MQ=255)                                                                                                                            gCTCCACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTg     >  1:923358/1‑140 (MQ=255)                                                                                                                               ccACGCAGGAGGCATTCTCGCCGCCACGTTTACTCATATTCCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGTATCCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCt  <  2:870390/140‑1 (MQ=255)                                                                                                                               ccACGCAGGAGGCATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCt  <  2:452151/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 CGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCTTTGATGGCTTCATCGCGAATCGAATCTTTAATACCCACAACCACGATGTCCTGAATTTTCGGGTGCGCGGCGATAATATTTTCCAGCTCCACGCAGGAGACATTCTCGCCGCCACGTTTAATCATATTGCAGCGGCGATCGACGAAATAAAAAAAGTCCTCTTCGTCGCGGTATCCGGTATCGCCGGTATGCAGCCAGCCATCGGCTTCCAGCACTTTCGCAGTGGCT  >  NC_000913/36396‑36661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑