Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	665,853	A→G	V154A (GTG→GCG)	mrdB ←	SEDS family protein MrdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	665,853	0	A	G	100.0%	254.5 / NA	70	V154A (GTG→GCG)	mrdB	SEDS family protein MrdB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/33);  total (37/33)

ACCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGAAAACG  >  NC_000913/665716‑665989                                                                                                                                          |                                                                                                                                         aCCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAgccgcc                                                                                                                                        >  2:516412/1‑140 (MQ=255)   cacaGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCcag                                                                                                                                      >  2:125382/1‑140 (MQ=255)   cacaGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCcag                                                                                                                                      >  1:277392/1‑140 (MQ=255)     caGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCcagca                                                                                                                                    >  1:822527/1‑140 (MQ=255)        aaTCGGAATGAACGCCGCTACCAGCACTACTGTGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCg                                                                                                                                 <  2:708208/140‑1 (MQ=255)         aTCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGt                                                                                                                                <  2:822527/140‑1 (MQ=255)         aTCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGt                                                                                                                                <  2:536425/140‑1 (MQ=255)         aTCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGt                                                                                                                                <  2:569256/140‑1 (MQ=255)           cGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCg                                                                                                                                 <  2:391866/137‑1 (MQ=255)           cGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCg                                                                                                                                 >  1:391866/1‑137 (MQ=255)             gAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCagcagc                                                                                                                                  >  1:726133/1‑134 (MQ=255)             gAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCagcagc                                                                                                                                  <  2:726133/134‑1 (MQ=255)              aaTGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCa                                                                                                                           <  1:125382/140‑1 (MQ=255)                tGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAAGGAGGATTAATGTTCCCAGGTCAGGATGTGCAGCCGCCAGCAGCGTGGGCATa                                                                                                                         <  2:708924/140‑1 (MQ=255)                tGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATa                                                                                                                         <  1:516412/140‑1 (MQ=255)                tGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATa                                                                                                                         <  1:68941/140‑1 (MQ=255)                 gAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATaa                                                                                                                        <  1:534461/140‑1 (MQ=255)                 gAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATaa                                                                                                                        <  2:60746/140‑1 (MQ=255)                 gAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATaa                                                                                                                        <  1:111535/140‑1 (MQ=255)                     gccgcTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAatat                                                                                                                    <  1:585123/140‑1 (MQ=255)                        gcTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAg                                                                                                                 >  1:188734/1‑140 (MQ=255)                            ccAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATAACAGCAcc                                                                                                             <  2:453412/140‑1 (MQ=255)                                   tactGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGa                                                                                                      >  2:883488/1‑140 (MQ=255)                                    actGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGAt                                                                                                     >  1:275329/1‑140 (MQ=255)                                          aCGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAgcg                                                                                                         <  1:425433/130‑1 (MQ=255)                                          aCGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAgcg                                                                                                         >  2:425433/1‑130 (MQ=255)                                          aCGCCAATCAGACGCCAGCTAAGCCCAGACAGCAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCAGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGTCAgt                                                                                               >  2:628130/1‑140 (MQ=255)                                           cGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAgtg                                                                                              >  1:417599/1‑140 (MQ=255)                                           cGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAgtg                                                                                              >  1:795301/1‑140 (MQ=255)                                             ccAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAgtg                                                                                              >  2:771501/1‑138 (MQ=255)                                             ccAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAgtg                                                                                              <  1:771501/138‑1 (MQ=255)                                                       gCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGAt                                                                                  >  2:112976/1‑140 (MQ=255)                                                                   cAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACg                                                                      <  2:277392/140‑1 (MQ=255)                                                                     gagagGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTc                                                                    >  2:878227/1‑140 (MQ=255)                                                                       gagGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTcgc                                                                  <  1:691080/140‑1 (MQ=255)                                                                           aaCAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGtt                                                              >  2:222386/1‑140 (MQ=255)                                                                                     aCAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAgcgc                                                    >  2:969791/1‑140 (MQ=255)                                                                                     aCAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAgcgc                                                    >  2:419676/1‑140 (MQ=255)                                                                                      cAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAgcgcg                                                   <  1:878227/140‑1 (MQ=255)                                                                                        gACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCa                                                 >  1:750588/1‑140 (MQ=255)                                                                                          ccGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAgcgcg                                                   >  2:196743/1‑136 (MQ=255)                                                                                          ccGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAgcgcg                                                   <  1:196743/136‑1 (MQ=255)                                                                                          ccGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAAc                                               >  1:443405/1‑140 (MQ=255)                                                                                          ccGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAAc                                               >  2:11012/1‑140 (MQ=255)                                                                                          ccGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAAc                                               >  2:330766/1‑140 (MQ=255)                                                                                          ccGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAAc                                               >  2:917118/1‑140 (MQ=255)                                                                                           cGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAAcc                                              <  1:969791/140‑1 (MQ=255)                                                                                           cGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAAcc                                              <  2:750588/140‑1 (MQ=255)                                                                                             gAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCAt                                            >  2:38052/1‑140 (MQ=255)                                                                                             gAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCAt                                            >  2:508473/1‑140 (MQ=255)                                                                                               aaGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCa                                          <  1:883488/140‑1 (MQ=255)                                                                                                      aCGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTAc                                   <  1:508473/140‑1 (MQ=255)                                                                                                      aCGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTAc                                   <  1:38052/140‑1 (MQ=255)                                                                                                       cGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACg                                  >  2:789747/1‑140 (MQ=255)                                                                                                            aTTGATGTTCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTAt                             >  2:69569/1‑140 (MQ=255)                                                                                                                   ttCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTAtt                            <  2:831908/134‑1 (MQ=255)                                                                                                                   ttCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTAtt                            >  1:831908/1‑134 (MQ=255)                                                                                                                   ttCCCAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCa                      >  1:4859/1‑140 (MQ=255)                                                                                                                     cccAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTAt                             >  1:803380/1‑131 (MQ=255)                                                                                                                     cccAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTAt                             <  2:377747/131‑1 (MQ=255)                                                                                                                     cccAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTAt                             <  2:803380/131‑1 (MQ=255)                                                                                                                     cccAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTAt                             >  1:377747/1‑131 (MQ=255)                                                                                                                     cccAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTAGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAAt                    >  2:569776/1‑140 (MQ=255)                                                                                                                      ccAGGTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAAtt                   >  1:636851/1‑140 (MQ=255)                                                                                                                          gTCAGGCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCg               <  1:917118/140‑1 (MQ=255)                                                                                                                               gCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCaa                     <  1:477726/129‑1 (MQ=255)                                                                                                                               gCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCaa                     >  2:477726/1‑129 (MQ=255)                                                                                                                               gCTGTGCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGc          >  2:536563/1‑140 (MQ=255)                                                                                                                                    gCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGaaa     <  2:188734/140‑1 (MQ=255)                                                                                                                                    gCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGaaa     <  1:69569/140‑1 (MQ=255)                                                                                                                                    gCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGaaa     >  2:106171/1‑140 (MQ=255)                                                                                                                                    gCAGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGaaa     >  2:286974/1‑140 (MQ=255)                                                                                                                                      aGCCGCCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCGACGATGGCGGGCAAACGTCGCGGTTGATAAAGTGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGAAAAc   <  1:628130/140‑1 (MQ=255)                                                                                                                                       gccgccAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGAAAACg  <  1:39331/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ACCACAGAATCGGAATGAACGCCGCTACCAGCACTACTGCGACGCCAATCAGACGCCAGCTAAGGCCAGAGAGGAACAGTACAAACAGACCGGAAAGCGCAACGAGGATTGATGTTCCCAGGTCAGGCTGTGCAGCCACCAGCAGCGTGGGCATAAATATCAGCACCAGCGCGATGCCAGTGTTCTTCAACGATGGCGGGCAAACGTCGCGGTTGATAAAGCGCGCAACCATCAGTGGTACGGCTATTTTGGCAATTTCCGACGGCTGAAAACG  >  NC_000913/665716‑665989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑