Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 704,616 A→G T225A (ACC→GCC)  nagE → N‑acetylglucosamine specific PTS enzyme IIABC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913704,6160AG100.0% 117.5 / NA 35T225A (ACC→GCC) nagEN‑acetylglucosamine specific PTS enzyme IIABC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/18);  total (17/18)

ACCGTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGTATGCTGCTTTCTGTTGCTGTT  >  NC_000913/704485‑704750
                                                                                                                                   |                                                                                                                                      
aCCGTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCgggg                                                                                                                                <  1:785702/140‑1 (MQ=255)
 ccGTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGa                                                                                                                               <  2:500620/140‑1 (MQ=255)
     ctgctgATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGcgccgc                                                                                                                                    >  1:259099/1‑131 (MQ=255)
     ctgctgATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGcgccgc                                                                                                                                    <  2:259099/131‑1 (MQ=255)
     ctgctgATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGtt                                                                                                                           <  1:867235/140‑1 (MQ=255)
      tgctgATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACTAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGGTGTTc                                                                                                                          >  1:574299/1‑140 (MQ=255)
      tgctgATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTTCACGGTGAGATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGGTGttt                                                                                                                          >  2:917997/1‑139 (MQ=255)
           aTCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGTTTTCCACCGTGACATTAACCGCTTCTCTTCCCGCGACGGCGCCCCCGGGATGTTCATGTc                                                                                                                     >  2:785702/1‑140 (MQ=255)
               cAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACTAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGc                                                                                                                 <  2:574299/140‑1 (MQ=255)
                    ggTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGcttctt                                                                                                            <  1:917997/140‑1 (MQ=255)
                          cATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTTCACGGGGGCATTAAACGCTTCTATGCCGGGGACGGCGCCGCGGGGATGTTTATGTCCGGCTTTTTCCCGAt                                                                                                      >  2:34574/1‑140 (MQ=255)
                                    tGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGGTGTTCATGTCCGGCTTCTTCCCCATCATGATGttt                                                                                            >  2:853254/1‑139 (MQ=255)
                                      aaCACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACCGTGACCTTTACCGCCTCTATTCCGGGGGCCGCGCCGCGGGGGTGTTGATGTCCGGCTTCTTCCCCATCATGATGGTCgg                                                                                          >  2:717413/1‑140 (MQ=255)
                                       accccATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGt                                                                                         <  2:66765/137‑1 (MQ=255)
                                       acacCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGt                                                                                         <  2:289307/140‑1 (MQ=255)
                                          ccATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGTCGGTTTTCCAACGGGACAATAACCGCCTTTATGTCGGTGACGGGGCCGCGGGGGGGTTCATGTCCGGCCTCTTTCCGATCATGTTGTTCGGTCTg                                                                                      >  2:336757/1‑140 (MQ=255)
                                            aTCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGcc                                                                                    <  1:34574/140‑1 (MQ=255)
                                             tCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTCCACGGTGACATTAACCGCTTCTATGCCGGGGACGGCGCCGCGGGGGTGTTCATGTCCGGCTTCTTTCCCATCATGATGTTCGGGCTGCCg                                                                                   >  2:72116/1‑140 (MQ=255)
                                                ccTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTGTTCCCGGTCATGATGTTCGGTCTGCCgggg                                                                                >  2:602768/1‑139 (MQ=255)
                                                  tGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTTCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGGTGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCgggggg                                                                              >  2:225833/1‑137 (MQ=255)
                                                       ccAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGgcgc                                                                         <  1:225833/140‑1 (MQ=255)
                                                         aGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTg                                                                       >  2:92113/1‑140 (MQ=255)
                                                               gTGAATTCACCAACGCGGCGGGGACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATg                                                                 >  1:265914/1‑140 (MQ=255)
                                                               gTGAATTCACCAACGCGGCGGGGACGGGTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATg                                                                 >  1:393456/1‑140 (MQ=255)
                                                                       aCCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCgc                                                         <  1:602768/140‑1 (MQ=255)
                                                                           aCGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCgcagca                                                     <  1:72116/140‑1 (MQ=255)
                                                                            cgcgGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCAc                                                    <  1:336757/140‑1 (MQ=255)
                                                                                      aCGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGcg                                          <  1:92113/140‑1 (MQ=255)
                                                                                          ttttCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGcgtcgt                                      >  1:881119/1‑140 (MQ=255)
                                                                                           tttCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCgtcgtc                                     <  2:265914/140‑1 (MQ=255)
                                                                                                    tGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTg                            >  2:312653/1‑140 (MQ=255)
                                                                                                    tGACATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTg                            >  1:533789/1‑140 (MQ=255)
                                                                                                      aCATTAACCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGc                          >  1:821217/1‑140 (MQ=255)
                                                                                                            aCCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGt                              >  2:129342/1‑130 (MQ=255)
                                                                                                            aCCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGt                              <  1:129342/130‑1 (MQ=255)
                                                                                                            aCCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGTAtg                    <  1:312653/140‑1 (MQ=255)
                                                                                                            aCCGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGTAtg                    >  1:309092/1‑140 (MQ=255)
                                                                                                             ccGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGTAtgc                   >  2:756185/1‑140 (MQ=255)
                                                                                                              cGCTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGTAtgct                  >  1:211935/1‑140 (MQ=255)
                                                                                                                cTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCg                         <  1:819379/131‑1 (MQ=255)
                                                                                                                cTTCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCg                         >  2:819379/1‑131 (MQ=255)
                                                                                                                  tCTATGCCGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGTATGCTGCtt              >  2:153562/1‑140 (MQ=255)
                                                                                                                         cGGTGACGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGTATGCTGCTTTCTGTTg       <  1:717413/140‑1 (MQ=255)
                                                                                                                              aCGGCGCCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGTATGCTGCTTTCTGTTGCTGtt  >  2:751178/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                      
ACCGTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTCTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGTATGCTGCTTTCTGTTGCTGTT  >  NC_000913/704485‑704750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑