Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	782,659	A→G	D192G (GAC→GGC)	nadA →	quinolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	782,659	0	A	G	96.0%	174.3 / 0.2	50	coding (575/1044 nt)	nadA	quinolinate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (23/25);  minor base . (1/1);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

AAAGCGCGCGCAGATTGGGTGGTAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATT  >  NC_000913/782523‑782783                                                                                                                                         |                                                                                                                             aaaGCGCGCGCAGATTGGGTGGTAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCAt                                                                                                                           >  2:350582/1‑140 (MQ=255)  aaGCGCGCGCAGATTGGGTGGTAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCAtt                                                                                                                          <  1:369722/140‑1 (MQ=255)            aGATTGGGTGGTAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGc                                                                                                                <  1:457486/140‑1 (MQ=255)              aTTGGGTGGTAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAg                                                                                                              <  2:37140/140‑1 (MQ=255)              aTTGGGTGGTAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAg                                                                                                              <  2:858583/140‑1 (MQ=255)                tGGGTGGTAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAggg                                                                                                            <  1:74484/140‑1 (MQ=255)                tGGGTGGTAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAggg                                                                                                            <  2:591785/140‑1 (MQ=255)                tGGGTGGTAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAggg                                                                                                            <  1:643812/140‑1 (MQ=255)                    tggtAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGc                                                                                                         >  1:763678/1‑139 (MQ=255)                    tggtAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGc                                                                                                         <  2:763678/139‑1 (MQ=255)                        aaCTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTa                                                                                                    >  2:779654/1‑140 (MQ=255)                            tCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATtgt                                                                                                >  2:379353/1‑140 (MQ=255)                                               ttATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACt                                                                             <  1:379353/140‑1 (MQ=255)                                                tATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTa                                                                                  <  2:249373/134‑1 (MQ=255)                                                tATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTa                                                                                  >  1:249373/1‑134 (MQ=255)                                                tATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTc                                                                            >  1:101348/1‑140 (MQ=255)                                                tATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTc                                                                            <  2:705794/140‑1 (MQ=255)                                                        atcTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTa                                                                    >  2:439482/1‑140 (MQ=255)                                                         tcTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTaa                                                                   >  2:322916/1‑140 (MQ=255)                                                          cTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACGTCTGGGGCGTTACGTGCAGAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAAc                                                                  >  2:877911/1‑140 (MQ=255)                                                          cTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAGCAGACGGGTGGAG‑‑‑‑GCTATGCTGACAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGc                                                              >  2:866389/1‑140 (MQ=255)                                                           ttGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAAcc                                                                 <  1:439482/140‑1 (MQ=255)                                                                  tttGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGc                                                          >  1:782844/1‑140 (MQ=255)                                                                    tGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAAcc                                                                 <  1:912344/131‑1 (MQ=255)                                                                    tGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAAcc                                                                 >  2:912344/1‑131 (MQ=255)                                                                    tGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCaa                                                        >  2:699829/1‑140 (MQ=255)                                                                    tGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCaa                                                        >  2:187977/1‑140 (MQ=255)                                                                    tGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCaa                                                        >  2:425623/1‑140 (MQ=255)                                                                    tGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCaa                                                        >  2:927896/1‑140 (MQ=255)                                                                    tGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCaa                                                        >  2:263515/1‑140 (MQ=255)                                                                      ggTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCaaga                                                      >  2:156258/1‑140 (MQ=255)                                                                         gAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAagaaga                                                   <  1:425623/140‑1 (MQ=255)                                                                                  atcTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGa                                          <  1:263515/140‑1 (MQ=255)                                                                                  atcTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGa                                          <  1:699829/140‑1 (MQ=255)                                                                                   tcTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAG‑‑‑‑GCTATGCTGACAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGAtgctg                                     <  1:866389/140‑1 (MQ=255)                                                                                       ggCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGTATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGAtg                                        >  1:401518/1‑137 (MQ=255)                                                                                       ggCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGTATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGAtg                                        <  2:401518/137‑1 (MQ=255)                                                                                       ggCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGAtgctg                                     <  1:322916/140‑1 (MQ=255)                                                                                       ggCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGAtgctg                                     <  2:291223/140‑1 (MQ=255)                                                                                            ccGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATa                                <  1:350582/140‑1 (MQ=255)                                                                                            ccGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATa                                <  2:782844/140‑1 (MQ=255)                                                                                                         ggggCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAg                   >  1:964966/1‑140 (MQ=255)                                                                                                          gggCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGa                  >  1:321547/1‑140 (MQ=255)                                                                                                          gggCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGa                  >  2:375679/1‑140 (MQ=255)                                                                                                            gCGTTACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGAAt                <  1:779654/140‑1 (MQ=255)                                                                                                               ttACGTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGAATcac             >  1:760610/1‑140 (MQ=255)                                                                                                                   gTGCAAAAACAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGAATCACcaca         <  2:964966/140‑1 (MQ=255)                                                                                                                       aaaaaCAGACGGGTGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCt     <  2:101348/140‑1 (MQ=255)                                                                                                                                    tGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTAtt  >  1:527633/1‑130 (MQ=255)                                                                                                                                    tGGAGGCATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTAtt  <  2:527633/130‑1 (MQ=255)                                                                                                                                         |                                                                                                                             AAAGCGCGCGCAGATTGGGTGGTAACTTCAAGCATTGCCGTCGAACTTATTGATCATCTTGATAGTTTGGGTGAAAAAATCATCTGGGCACCCGACAAACATCTGGGGCGTTACGTGCAAAAACAGACGGGTGGAGACATTCTATGCTGGCAGGGTGCCTGTATTGTGCATGATGAATTTAAGACTCAGGCGTTAACCCGCTTGCAAGAAGAATACCCGGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATT  >  NC_000913/782523‑782783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑