Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	897,325	T→C	I81T (ATC→ACC)	potI →	putrescine ABC transporter membrane subunit PotI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	897,325	0	T	C	100.0%	136.5 / NA	38	I81T (ATC→ACC)	potI	putrescine ABC transporter membrane subunit PotI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/20);  total (18/20)

AACAGCTCGAAGCTGGTGACGGTGTGGGCCGGCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGT  >  NC_000913/897189‑897447                                                                                                                                         |                                                                                                                           aaCAGCTCGAAGCTGGTGACGGTGTGGGCCGGCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCt                                                                                                                         <  2:563357/140‑1 (MQ=255)                gTGACGGTGTGGGCCGGCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTgcggc                                                                                                          <  2:68573/140‑1 (MQ=255)                       tgtgGGCCGGCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGgtggtg                                                                                                   >  2:273001/1‑140 (MQ=255)                            gCCGGCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGtggtgctggt                                                                                              <  1:966608/140‑1 (MQ=255)                            gCCGGCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGtggtgctggt                                                                                              <  1:612650/140‑1 (MQ=255)                                gCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGtggtgctgg                                                                                               >  2:850242/1‑135 (MQ=255)                                gCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGtggtgctgg                                                                                               <  1:850242/135‑1 (MQ=255)                                gCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGtgggggtggggggg                                                                                          >  2:791309/1‑135 (MQ=255)                                gCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGtgggggtggggggc                                                                                          >  2:499139/1‑135 (MQ=255)                                 cTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGGACTATTGCGGCGGGGGTGGTGGTgggcc                                                                                         >  2:874311/1‑139 (MQ=255)                                 cTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCt                                                                                         >  1:600603/1‑140 (MQ=255)                                 cTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGGGGTGGGgcggt                                                                                         >  2:261661/1‑138 (MQ=255)                                              tGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTcg                                                                            >  2:10778/1‑140 (MQ=255)                                                        aGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGGGGTGGTGCGCCTTTGCAGGGTTTGCGGATCaaaa                                                                  >  1:761651/1‑139 (MQ=255)                                                        aGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGGGCTGGGGCGCTTTTGCAGGGTTCGCGGATCaaaa                                                                  >  1:499562/1‑139 (MQ=255)                                                          ttATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGGGGTGGGGGGCTTTTGCAGGGTTCGCGGATCAAAAgg                                                                >  1:763404/1‑140 (MQ=255)                                                          ttATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGGGGGGCTGGGGGGCCTTTGCAGGGTTTGCGGGTCAAAAgg                                                                >  2:789487/1‑140 (MQ=255)                                                            aTTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCt                                                              <  1:789487/140‑1 (MQ=255)                                                            aTTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCt                                                              <  2:972988/140‑1 (MQ=255)                                                            aTTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCt                                                              <  1:261661/140‑1 (MQ=255)                                                            aTTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCt                                                              <  2:703937/140‑1 (MQ=255)                                                             ttGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGCAGTGCTGGAGCGCGTTTGCTGGGTTCGCGGATCTAAAGGcat                                                             >  2:168645/1‑138 (MQ=255)                                                               gcgcGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTg                                                           <  2:397700/140‑1 (MQ=255)                                                                 gcgatgacgcgatgaTGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGcc                                                         <  1:273001/140‑1 (MQ=255)                                                                 gcgatgacgcgatgaTGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGcc                                                         <  2:600603/140‑1 (MQ=255)                                                                    atgacgcgatgaTGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATgg                                                                <  2:279067/130‑1 (MQ=255)                                                                    atgacgcgatgaTGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATgg                                                                >  1:279067/1‑130 (MQ=255)                                                                                tgaGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACcgcg                                          <  1:10778/140‑1 (MQ=255)                                                                                   gTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGcgccg                                       <  1:791309/140‑1 (MQ=255)                                                                                   gTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGcgccg                                       <  1:499139/140‑1 (MQ=255)                                                                                   gTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGGGGTGGGGCGCTTTGGCAGGGTTTGCGGGTCAAATGGCTTTTCCTTTTTGATCACCGcgccc                                       >  2:29403/1‑139 (MQ=255)                                                                                           ggttTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGGTTTGCCGATCAAAAGGCTTTTCCTTTTTGATCACCCCGCCGCTGGGaaa                               >  2:595910/1‑139 (MQ=255)                                                                                              ttAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCAGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGcc                            <  1:131246/140‑1 (MQ=255)                                                                                                           ttGCGGCCTGTGCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGGGCGCTTTTGCAGGGTTCGCGGGTCAAAAGGCTTTTCCTTTATGATAACCCCGCCGCTGGTAATGCCAGATGTCATCAcc               >  2:844161/1‑139 (MQ=255)                                                                                                                    gtgCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCg      <  2:256683/140‑1 (MQ=255)                                                                                                                    gtgCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCg      <  1:29403/140‑1 (MQ=255)                                                                                                                     tgCGGCGACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGGGGGCTTTGGGAGGGTTTGCGGGTCAAAAGGGTTTTGCTTTTTTATCACCGCCCCCCCGGGAAAGCCCGAAGTCATCACCGGGTTTTCGc     >  2:462111/1‑140 (MQ=255)                                                                                                                        ggcgACGGCGGCGGCGACCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCtgt  <  1:877406/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                           AACAGCTCGAAGCTGGTGACGGTGTGGGCCGGCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGT  >  NC_000913/897189‑897447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑