Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 960,067 A→G Q419R (CAG→CGG)  aroA → 3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913960,0670AG100.0% 253.1 / NA 69Q419R (CAG→CGG) aroA3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/31);  total (38/31)

AACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCG  >  NC_000913/959934‑960202
                                                                                                                                     |                                                                                                                                       
aaCTTTGCCGAGATCGCGACATACAATGATCACCGGATGGTGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGgc                                                                                                                                   <  2:613272/140‑1 (MQ=255)
               gcgACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGc                                                                                                                    >  1:836275/1‑140 (MQ=255)
                  aCATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGc                                                                                                                 <  2:304767/140‑1 (MQ=255)
                   cATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGcc                                                                                                                <  1:150179/140‑1 (MQ=255)
                   cATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGcc                                                                                                                <  2:836275/140‑1 (MQ=255)
                    aTACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCt                                                                                                               <  2:540320/140‑1 (MQ=255)
                             tCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaaca                                                                                                      >  2:700919/1‑140 (MQ=255)
                             tCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaaca                                                                                                      <  1:700919/140‑1 (MQ=255)
                              cACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaa                                                                                                     <  2:234255/140‑1 (MQ=255)
                               aCCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaac                                                                                                    >  2:192942/1‑140 (MQ=255)
                               aCCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaac                                                                                                    <  2:259652/140‑1 (MQ=255)
                               aCCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaac                                                                                                    >  2:570874/1‑140 (MQ=255)
                               aCCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaac                                                                                                    >  2:519873/1‑140 (MQ=255)
                               aCCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaac                                                                                                    >  2:493526/1‑140 (MQ=255)
                               aCCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaa                                                                                                     >  1:433216/1‑139 (MQ=255)
                               aCCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaa                                                                                                     <  2:433216/139‑1 (MQ=255)
                                ccGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACAGCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACg                                                                                                   >  2:878371/1‑140 (MQ=255)
                                ccGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACg                                                                                                   >  2:892571/1‑140 (MQ=255)
                                  ggATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaa                                                                                                     <  2:481447/136‑1 (MQ=255)
                                  ggATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaa                                                                                                     >  1:481447/1‑136 (MQ=255)
                                   gATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGc                                                                                                >  2:908942/1‑140 (MQ=255)
                                    aTGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaa                                                                                                     >  1:773407/1‑134 (MQ=255)
                                    aTGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGaacaa                                                                                                     <  2:773407/134‑1 (MQ=255)
                                      ggCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCaat                                                                                             >  1:840006/1‑140 (MQ=255)
                                      ggCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCaat                                                                                             >  1:457561/1‑140 (MQ=255)
                                        cGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCaataa                                                                                           >  1:967256/1‑140 (MQ=255)
                                           tgtgTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCaataaata                                                                                        >  1:262624/1‑140 (MQ=255)
                                           tgtgTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCaataaata                                                                                        >  1:334636/1‑140 (MQ=255)
                                            gtgtTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAg                                                                                       <  2:389742/140‑1 (MQ=255)
                                             tgtTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGc                                                                                      >  2:55500/1‑140 (MQ=255)
                                             tgtTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGc                                                                                      >  2:2818/1‑140 (MQ=255)
                                              gtTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGcc                                                                                     <  1:908942/140‑1 (MQ=255)
                                                    cGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAAtct                                                                               <  2:915919/140‑1 (MQ=255)
                                                       tggtggCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAAtctttc                                                                            >  1:448838/1‑140 (MQ=255)
                                                        ggtggCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAAtctttct                                                                           <  1:878371/140‑1 (MQ=255)
                                                         gtggCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAAtctttctt                                                                          >  2:199825/1‑140 (MQ=255)
                                                               ttGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCaa                                                                    <  2:840006/140‑1 (MQ=255)
                                                                       tACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCgg                                                            >  1:411365/1‑140 (MQ=255)
                                                                        acacCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGc                                                           >  1:534919/1‑140 (MQ=255)
                                                                         cacCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGca                                                          >  1:346807/1‑140 (MQ=255)
                                                                              gTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTg                                                     >  2:103368/1‑140 (MQ=255)
                                                                                gACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTc                                                   >  2:270905/1‑140 (MQ=255)
                                                                                    aTTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCg                                               >  2:472765/1‑140 (MQ=255)
                                                                                     ttCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGt                                              >  2:277129/1‑140 (MQ=255)
                                                                                         tGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGttttt                                          >  2:646396/1‑140 (MQ=255)
                                                                                            tCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGtttttttt                                       <  2:334636/140‑1 (MQ=255)
                                                                                              ccAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCg                                     <  1:192942/140‑1 (MQ=255)
                                                                                              ccAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCg                                     <  1:472765/140‑1 (MQ=255)
                                                                                               cAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCgg                                    >  1:412474/1‑140 (MQ=255)
                                                                                               cAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCgg                                    >  2:516038/1‑140 (MQ=255)
                                                                                                aaaTGCACGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGa                                   <  1:270905/140‑1 (MQ=255)
                                                                                                      aCGGCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTtgt                             <  2:457561/140‑1 (MQ=255)
                                                                                                         gCCAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAg                          <  1:516038/140‑1 (MQ=255)
                                                                                                          ccAAAACATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGt                         >  2:630360/1‑140 (MQ=255)
                                                                                                             aaaCATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCAt                      >  1:616164/1‑140 (MQ=255)
                                                                                                             aaaCATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCAt                      >  1:52752/1‑140 (MQ=255)
                                                                                                               aCATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCAttt                    <  2:793602/140‑1 (MQ=255)
                                                                                                               aCATTTCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCAttt                    <  2:534919/140‑1 (MQ=255)
                                                                                                                   ttCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGAt                <  2:448838/140‑1 (MQ=255)
                                                                                                                   ttCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGAt                <  1:892571/140‑1 (MQ=255)
                                                                                                                    tCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGAtt               >  2:600308/1‑140 (MQ=255)
                                                                                                                    tCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGAtt               >  1:790124/1‑140 (MQ=255)
                                                                                                                    tCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGAtt               >  2:19053/1‑140 (MQ=255)
                                                                                                                    tCCGGATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGCGAGTCATTTTGAtt               <  1:646396/140‑1 (MQ=255)
                                                                                                                        gATTATTTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCAATTTGATTAATg           >  2:548587/1‑140 (MQ=255)
                                                                                                                          ttattTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGt         <  1:600308/140‑1 (MQ=255)
                                                                                                                          ttattTCGAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGt         <  2:305902/140‑1 (MQ=255)
                                                                                                                                 gAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCg  <  1:493526/140‑1 (MQ=255)
                                                                                                                                 gAGCGGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCg  <  1:199825/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
AACTTTGCCGAGATCGCGACATACAATGATCACCGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCG  >  NC_000913/959934‑960202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑