Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	977,782	A→G	N486S (AAC→AGC)	mukB →	chromosome partitioning protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	977,782	0	A	G	100.0%	91.8 / NA	26	N486S (AAC→AGC)	mukB	chromosome partitioning protein MukB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/10);  total (16/10)

CTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAG  >  NC_000913/977655‑977918                                                                                                                                |                                                                                                                                         cTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGgc                                                                                                                              <  2:280855/140‑1 (MQ=255)        ccTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAAc                                                                                                                       <  2:812582/140‑1 (MQ=255)                                  aCTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACt                                                                                             >  1:442872/1‑140 (MQ=255)                                                ctctctTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGTACTATTGCGCGAAgggg                                                                               >  2:876192/1‑140 (MQ=255)                                                ctctctTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAgggg                                                                               >  2:812022/1‑140 (MQ=255)                                                   tctTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCg                                                                            <  2:184063/140‑1 (MQ=255)                                                          cAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCg                                                                     <  2:808865/140‑1 (MQ=255)                                                           aGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGt                                                                    >  1:921122/1‑140 (MQ=255)                                                             aaaaTGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCa                                                                  >  1:694516/1‑140 (MQ=255)                                                             aaaaTGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCa                                                                  >  1:899493/1‑140 (MQ=255)                                                                  gAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTgg                                                             <  2:694516/140‑1 (MQ=255)                                                                       tGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCagag                                                        >  1:418932/1‑140 (MQ=255)                                                                       tGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCagag                                                        >  1:495161/1‑140 (MQ=255)                                                                        ggCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGc                                                       >  2:291209/1‑140 (MQ=255)                                                                         gcgcAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCa                                                      >  1:931509/1‑140 (MQ=255)                                                                                 cgcgCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGc                                              <  1:812022/140‑1 (MQ=255)                                                                                   cgcACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCg                                            >  1:966897/1‑140 (MQ=255)                                                                                    gcACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGc                                           >  2:206520/1‑140 (MQ=255)                                                                                      acaGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCg                                            >  2:14376/1‑137 (MQ=255)                                                                                      acaGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCg                                            <  1:14376/137‑1 (MQ=255)                                                                                                       gCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGa                        >  2:34538/1‑140 (MQ=255)                                                                                                       gCTTATCAGCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGa                        >  1:761227/1‑140 (MQ=255)                                                                                                               gCTGGTGGTGGCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAAc                <  2:899493/140‑1 (MQ=255)                                                                                                                        ggCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTAc       >  1:869518/1‑140 (MQ=255)                                                                                                                         gCAATCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTAcg      <  2:495161/140‑1 (MQ=255)                                                                                                                             tCAGCGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGag  <  1:206520/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                         CTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAG  >  NC_000913/977655‑977918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑