Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	54,578	A→G	V42A (GTT→GCT)	surA ←	peptidyl‑prolyl cis‑trans isomerase SurA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	54,578	0	A	G	100.0%	280.2 / NA	77	V42A (GTT→GCT)	surA	peptidyl‑prolyl cis‑trans isomerase SurA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/38);  total (39/38)

ATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCAT  >  NC_000913/54443‑54713                                                                                                                                        |                                                                                                                                        aTTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTc                                                                                                                                     >  2:732271/1‑140 (MQ=255) aTTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTc                                                                                                                                     >  1:928743/1‑140 (MQ=255) aTTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTc                                                                                                                                     >  2:537104/1‑140 (MQ=255)   tttCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGc                                                                                                                                   <  1:494529/140‑1 (MQ=255)         ccccATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCa                                                                                                                             >  1:578518/1‑140 (MQ=255)             aTCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCg                                                                                                                                    <  1:188336/129‑1 (MQ=255)             aTCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCg                                                                                                                                    >  2:188336/1‑129 (MQ=255)               cTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCacga                                                                                                                       <  1:732271/140‑1 (MQ=255)               cTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCacga                                                                                                                       <  2:149384/140‑1 (MQ=255)                 gCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCacgacg                                                                                                                     <  2:37686/140‑1 (MQ=255)                 gCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGACGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGGCGCTTTCCAGCacgacg                                                                                                                     >  2:876777/1‑140 (MQ=255)                       tgatTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTa                                                                                                               >  2:352382/1‑140 (MQ=255)                          tttGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTg                                                                                                            >  1:926704/1‑140 (MQ=255)                          tttGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTg                                                                                                            >  1:827233/1‑140 (MQ=255)                          tttGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACAACGCCGTTATTg                                                                                                            >  1:482610/1‑140 (MQ=255)                            tGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCa                                                                                                                          >  2:973345/1‑124 (MQ=255)                            tGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCa                                                                                                                          <  1:973345/124‑1 (MQ=255)                            tGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCa                                                                                                                          >  2:800865/1‑124 (MQ=255)                            tGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCa                                                                                                                          <  1:800865/124‑1 (MQ=255)                            tGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTgac                                                                                                          >  1:307541/1‑140 (MQ=255)                             gATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTgacg                                                                                                         <  1:537104/140‑1 (MQ=255)                             gATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTgacg                                                                                                         <  2:756203/140‑1 (MQ=255)                              aTCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTgacga                                                                                                        <  1:681400/140‑1 (MQ=255)                               tCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTgacgac                                                                                                       >  2:351419/1‑140 (MQ=255)                               tCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTgacgac                                                                                                       >  1:198954/1‑140 (MQ=255)                               tCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTgacgac                                                                                                       >  2:36968/1‑140 (MQ=255)                                    gATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTg                                                                                                  >  2:931685/1‑140 (MQ=255)                                    gATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTg                                                                                                  >  1:612269/1‑140 (MQ=255)                                      tCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCg                                                                                                <  2:926704/140‑1 (MQ=255)                                       cAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGa                                                                                               <  1:36968/140‑1 (MQ=255)                                       cAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGa                                                                                               <  1:351419/140‑1 (MQ=255)                                        aaaCGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGAc                                                                                              >  1:349707/1‑140 (MQ=255)                                         aaCGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACt                                                                                             <  1:633882/140‑1 (MQ=255)                                             ttCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAG‑TTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATc                                                                                        >  1:171370/1‑140 (MQ=255)                                              tCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATc                                                                                        >  1:420496/1‑140 (MQ=255)                                                cATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGa                                                                                      <  2:827233/140‑1 (MQ=255)                                                cATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGa                                                                                      <  2:744774/140‑1 (MQ=255)                                                cATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGa                                                                                      <  2:420496/140‑1 (MQ=255)                                                cATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCATCGCTTTCCAGCACGACGCCGGTATTGACGACGGCTGCGACTTTATCGa                                                                                      >  2:721374/1‑140 (MQ=255)                                                    aTTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTAc                                                                                  <  2:715119/140‑1 (MQ=255)                                                        ggtggCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTgg                                                                              >  1:56722/1‑140 (MQ=255)                                                        ggtggCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTgg                                                                              >  2:806985/1‑140 (MQ=255)                                                          tggCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTgggg                                                                            <  2:349707/140‑1 (MQ=255)                                                             cgcAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGc                                                                         >  1:659405/1‑140 (MQ=255)                                                             cgcAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGc                                                                         >  1:348016/1‑140 (MQ=255)                                                               cAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAg                                                                       <  2:382931/140‑1 (MQ=255)                                                               cAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAg                                                                       >  1:636226/1‑140 (MQ=255)                                                               cAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAg                                                                       >  1:105709/1‑140 (MQ=255)                                                               cAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAg                                                                       >  1:642764/1‑140 (MQ=255)                                                               cAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACAACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAg                                                                       <  2:482610/140‑1 (MQ=255)                                                               cAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCAGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAg                                                                       <  2:578518/140‑1 (MQ=255)                                                                  cgtcgcgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGa                                                                    <  1:352382/140‑1 (MQ=255)                                                                   gtcgcgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGaa                                                                   >  2:640773/1‑140 (MQ=255)                                                                       cgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTg                                                               <  2:755506/140‑1 (MQ=255)                                                                       cgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTg                                                               <  2:307541/140‑1 (MQ=255)                                                                        gtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTgg                                                              <  2:612269/140‑1 (MQ=255)                                                                           atcaGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTAt                                                           >  1:57735/1‑140 (MQ=255)                                                                             caGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTc                                                         <  2:57735/140‑1 (MQ=255)                                                                                  aGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGAt                                                    <  2:642764/140‑1 (MQ=255)                                                                                  aGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGAt                                                    <  1:640773/140‑1 (MQ=255)                                                                                       ctgcCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATgg                                               <  2:636226/140‑1 (MQ=255)                                                                                          ccttgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGa                                            >  2:6993/1‑140 (MQ=255)                                                                                          ccttgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGa                                            <  2:56722/140‑1 (MQ=255)                                                                                                    gcagcGTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAag                                  >  1:702342/1‑140 (MQ=255)                                                                                                         gTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCg                             >  2:680021/1‑140 (MQ=255)                                                                                                         gTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCg                             >  1:620929/1‑140 (MQ=255)                                                                                                         gTTCAGTTTTACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCg                             <  2:620929/140‑1 (MQ=255)                                                                                                               ttttACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAagcag                               <  2:986424/132‑1 (MQ=255)                                                                                                               ttttACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAagcag                               >  1:986424/1‑132 (MQ=255)                                                                                                                tttACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCa                      <  2:928743/140‑1 (MQ=255)                                                                                                                 ttACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAg                     <  2:702342/140‑1 (MQ=255)                                                                                                                 ttACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAg                     <  1:876777/140‑1 (MQ=255)                                                                                                                  tACCGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGt                    >  2:630160/1‑140 (MQ=255)                                                                                                                    ccGACTGCATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGttc                  >  1:674478/1‑140 (MQ=255)                                                                                                                           cATTAATCCATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATAc           <  2:674478/140‑1 (MQ=255)                                                                                                                                   cATCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCa   >  1:825629/1‑140 (MQ=255)                                                                                                                                    aTCAGCGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCAt  <  2:825629/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATTTTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCAT  >  NC_000913/54443‑54713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑