Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,159,060	A→G	T398A (ACC→GCC)	ptsG →	glucose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,060	0	A	G	100.0%	213.9 / NA	61	T398A (ACC→GCC)	ptsG	glucose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/31);  total (30/31)

TGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAAC  >  NC_000913/1158929‑1159195                                                                                                                                    |                                                                                                                                        tgtgGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGaa                                                                                                                                 >  1:609653/1‑140 (MQ=255)   tgGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAAt                                                                                                                               >  1:652818/1‑140 (MQ=255)           cccGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAg                                                                                                                                     >  2:453738/1‑126 (MQ=255)           cccGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAg                                                                                                                                     <  1:453738/126‑1 (MQ=255)           cccGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCgg                                                                                                                       >  1:497685/1‑140 (MQ=255)                   tcgGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGtt                                                                                                               >  2:56162/1‑140 (MQ=255)                   tcgGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGtt                                                                                                               <  1:667003/140‑1 (MQ=255)                     ggTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCAc                                                                                                                          >  2:921096/1‑127 (MQ=255)                     ggTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCAc                                                                                                                          <  1:921096/127‑1 (MQ=255)                              tATGCGGTTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTtgg                                                                                                    >  2:802626/1‑140 (MQ=255)                                tGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTtggtg                                                                                                  <  2:652818/140‑1 (MQ=255)                                 gCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTtggtgg                                                                                                 >  2:579514/1‑140 (MQ=255)                                 gCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTtggtgg                                                                                                 >  1:609973/1‑140 (MQ=255)                                      tGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTggtggt                                                                                                <  1:404409/136‑1 (MQ=255)                                      tGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTggtggt                                                                                                >  2:404409/1‑136 (MQ=255)                                      tGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAg                                                                                            >  2:721391/1‑140 (MQ=255)                                         ttACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGaaa                                                                                         <  1:56162/140‑1 (MQ=255)                                          tactacACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGaaaa                                                                                        <  2:609973/140‑1 (MQ=255)                                          tactacACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGaaaa                                                                                        >  2:367725/1‑140 (MQ=255)                                               cacCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTa                                                                                   >  2:332959/1‑140 (MQ=255)                                               cacCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTa                                                                                   >  2:188639/1‑140 (MQ=255)                                               cacCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTa                                                                                   >  1:453921/1‑140 (MQ=255)                                                  cATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTAc                                                                                  >  1:503645/1‑138 (MQ=255)                                                  cATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTAc                                                                                  <  2:503645/138‑1 (MQ=255)                                                    tCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAAc                                                                              >  1:525003/1‑140 (MQ=255)                                                    tCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAAc                                                                              >  1:230023/1‑140 (MQ=255)                                                      ttCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCt                                                                            <  2:525003/140‑1 (MQ=255)                                                      ttCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCt                                                                            <  2:453921/140‑1 (MQ=255)                                                      ttCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCt                                                                            <  1:407036/140‑1 (MQ=255)                                                       tCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTc                                                                           >  2:295982/1‑140 (MQ=255)                                                           cgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACg                                                                       <  1:802626/140‑1 (MQ=255)                                                           cgTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACg                                                                       <  1:585744/140‑1 (MQ=255)                                                            gTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGc                                                                      <  2:609653/140‑1 (MQ=255)                                                            gTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGc                                                                      <  1:295982/140‑1 (MQ=255)                                                            gTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGc                                                                      >  2:827831/1‑140 (MQ=255)                                                                  aTTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATg                                                                   <  2:665758/137‑1 (MQ=255)                                                                  aTTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATg                                                                   >  1:665758/1‑137 (MQ=255)                                                                  aTTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTAt                                                                >  1:470035/1‑140 (MQ=255)                                                                   ttAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTAtt                                                               <  2:230023/140‑1 (MQ=255)                                                                       aGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTAccc                                                           >  2:386035/1‑140 (MQ=255)                                                                            tGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACg                                                                       >  2:627872/1‑123 (MQ=255)                                                                            tGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACg                                                                       <  1:627872/123‑1 (MQ=255)                                                                            tGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTg                                                      <  1:188639/140‑1 (MQ=255)                                                                            tGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTg                                                      >  2:682705/1‑140 (MQ=255)                                                                              gATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTgcg                                                    >  1:465567/1‑140 (MQ=255)                                                                                 cTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGt                                                 >  1:816273/1‑140 (MQ=255)                                                                                    aaaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAg                                              <  2:885216/140‑1 (MQ=255)                                                                                    aaaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAg                                              <  2:816273/140‑1 (MQ=255)                                                                                        cGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGtt                                          >  1:698041/1‑140 (MQ=255)                                                                                         gCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTg                                         >  2:890942/1‑140 (MQ=255)                                                                                              gTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGCCTGCGCGTCAGCGTTGCTGAt                                    <  2:698041/140‑1 (MQ=255)                                                                                                  tGAAGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATgtgt                                <  1:332959/140‑1 (MQ=255)                                                                                                     aGACGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTa                             <  2:813477/140‑1 (MQ=255)                                                                                                       aCGCGACTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTaaa                           <  1:827831/140‑1 (MQ=255)                                                                                                            aCTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                    >  2:444809/1‑126 (MQ=255)                                                                                                            aCTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                    <  1:444809/126‑1 (MQ=255)                                                                                                            aCTGAAGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGa                      <  1:386035/140‑1 (MQ=255)                                                                                                                 aGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAgg                 <  1:454633/140‑1 (MQ=255)                                                                                                                 aGATGCAAAAGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAgg                 <  1:216921/140‑1 (MQ=255)                                                                                                                         aaGCGACAGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTg         <  1:570030/140‑1 (MQ=255)                                                                                                                                aGGTGCCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAAc  <  1:474892/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        TGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAAC  >  NC_000913/1158929‑1159195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑