Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,190,712	A→G	G425G (GGT→GGC)	purB ←	adenylosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,190,712	0	A	G	100.0%	217.3 / NA	60	G425G (GGT→GGC)	purB	adenylosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/30);  total (30/30)

CAGGCCGGACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTT  >  NC_000913/1190577‑1190846                                                                                                                                        |                                                                                                                                       cAGGCCGGACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCAt                                                                                                                                    >  1:796180/1‑140 (MQ=255)    gCCGGACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGa                                                                                                                                 >  2:429784/1‑140 (MQ=255)     ccGGACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGAt                                                                                                                                >  2:118789/1‑140 (MQ=255)        gACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATaaa                                                                                                                             >  1:371378/1‑140 (MQ=255)         aCAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAAc                                                                                                                            >  2:676972/1‑140 (MQ=255)                           aCTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCg                                                                                                          <  2:881997/140‑1 (MQ=255)                                 aCGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAAcg                                                                                                    <  1:429784/140‑1 (MQ=255)                                    aGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACAGCTTCATGCCTTCGGCGTCAACgcgc                                                                                                 >  1:689174/1‑140 (MQ=255)                                       tttATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCaa                                                                                                      >  1:166156/1‑132 (MQ=255)                                       tttATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCaa                                                                                                      <  2:166156/132‑1 (MQ=255)                                       tttATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTa                                                                                              >  1:548364/1‑140 (MQ=255)                                          aTTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACcg                                                                                           <  1:676972/140‑1 (MQ=255)                                                 cTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCa                                                                                    <  2:76346/140‑1 (MQ=255)                                                  tcatcaACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAg                                                                                   <  1:727619/140‑1 (MQ=255)                                                         ccATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTc                                                                            >  1:682025/1‑140 (MQ=255)                                                                   aGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGt                                                                  >  1:550438/1‑140 (MQ=255)                                                                    gCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACACGCTTACCGCGAGTCAGCTCTTTCAGCt                                                                        <  1:771347/133‑1 (MQ=255)                                                                    gCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACACGCTTACCGCGAGTCAGCTCTTTCAGCt                                                                        >  2:771347/1‑133 (MQ=255)                                                                       cGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACgg                                                              <  2:689174/140‑1 (MQ=255)                                                                         aCCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGtt                                                            >  1:813980/1‑140 (MQ=255)                                                                             atatAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGttttt                                                         >  2:935737/1‑139 (MQ=255)                                                                             atatAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGttttt                                                         <  1:935737/139‑1 (MQ=255)                                                                                  gTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGc                                                   <  2:550438/140‑1 (MQ=255)                                                                                    tAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGttttt                                                         >  2:855927/1‑132 (MQ=255)                                                                                    tAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGttttt                                                         <  1:855927/132‑1 (MQ=255)                                                                                    tAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCa                                                 >  2:664653/1‑140 (MQ=255)                                                                                     aGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCAt                                                >  1:940912/1‑140 (MQ=255)                                                                                     aGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCAt                                                >  1:267654/1‑140 (MQ=255)                                                                                      gCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGACAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATa                                               >  2:340606/1‑140 (MQ=255)                                                                                       ccGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAg                                              >  1:556315/1‑140 (MQ=255)                                                                                       ccGGCGTCATCGCTTTCAGGCGGGCTTACTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAg                                              <  2:318716/140‑1 (MQ=255)                                                                                        cGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGc                                             >  1:902971/1‑140 (MQ=255)                                                                                        cGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGc                                             >  2:14901/1‑140 (MQ=255)                                                                                        cGGCGTCATCGCTTTCAGGCGGGCCTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGc                                             >  1:669877/1‑140 (MQ=255)                                                                                         ggCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCg                                            <  2:267654/140‑1 (MQ=255)                                                                                          gCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTGATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGa                                           >  2:672745/1‑140 (MQ=255)                                                                                            gTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACg                                         <  2:371378/140‑1 (MQ=255)                                                                                            gTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACg                                         <  2:682025/140‑1 (MQ=255)                                                                                                tCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATa                                     <  2:796180/140‑1 (MQ=255)                                                                                                 cGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATaa                                    <  2:813980/140‑1 (MQ=255)                                                                                                              gCTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTgg                       >  1:939791/1‑140 (MQ=255)                                                                                                               cTTTCTCTTCTTCTGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGt                      <  2:629388/140‑1 (MQ=255)                                                                                                                      ttcttctGGCTACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTcagcca               <  1:676133/140‑1 (MQ=255)                                                                                                                        cttctGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTcagccagc             >  1:770428/1‑140 (MQ=255)                                                                                                                         ttctGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCa            <  2:770428/140‑1 (MQ=255)                                                                                                                         ttctGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCa            <  2:548364/140‑1 (MQ=255)                                                                                                                         ttctGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCa            <  2:902971/140‑1 (MQ=255)                                                                                                                          tctGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCAc           >  2:960521/1‑140 (MQ=255)                                                                                                                          tctGGCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCAc           <  1:664653/140‑1 (MQ=255)                                                                                                                              gCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTcc       <  2:669877/140‑1 (MQ=255)                                                                                                                              gCAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTcc       <  2:360054/140‑1 (MQ=255)                                                                                                                               cAACGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTccc      <  1:672745/140‑1 (MQ=255)                                                                                                                                  cGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACt          <  2:169329/133‑1 (MQ=255)                                                                                                                                  cGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACt          >  1:819277/1‑133 (MQ=255)                                                                                                                                  cGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACt          <  2:819277/133‑1 (MQ=255)                                                                                                                                  cGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACt          >  1:169329/1‑133 (MQ=255)                                                                                                                                  cGCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGt   >  1:781168/1‑140 (MQ=255)                                                                                                                                   gCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCa            <  2:40898/130‑1 (MQ=255)                                                                                                                                   gCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCa            >  1:40898/1‑130 (MQ=255)                                                                                                                                   gCCAGGCCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGtt  <  2:556315/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CAGGCCGGACAGCATCGCCATCCGGCACTGATACGAGGTTTATTTCAGCTCATCAACCATCGTGATAGCTCGACCAATATAGTTAGCCGGCGTCATCGCTTTCAGGCGGGCTTTCTCTTCTTCTGGCAACGCCAGACCATCGATAAACTGCTTCATGCCTTCGGCGTCAACGCGCTTACCGCGAGTCAGCTCTTTCAGCTTCTCGTACGGTTTTTCGATGCCATAGCGACGCATAACTGTCTGGATTGGTTCAGCCAGCACTTCCCAGTT  >  NC_000913/1190577‑1190846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑