Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,261,465	A→G	G137G (GGT→GGC)	prs ←	ribose‑phosphate diphosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,465	0	A	G	100.0%	207.4 / NA	57	G137G (GGT→GGC)	prs	ribose‑phosphate diphosphokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/32);  total (25/32)

GCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCA  >  NC_000913/1261329‑1261600                                                                                                                                         |                                                                                                                                        gcTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGccc                                                                                                                                      >  1:459036/1‑140 (MQ=255)   ttAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTg                                                                                                                                    >  1:736424/1‑140 (MQ=255)      gCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAAt                                                                                                                                 <  2:459036/140‑1 (MQ=255)           aGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGtt                                                                                                                            <  1:941555/140‑1 (MQ=255)             cgcgGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCa                                                                                                                          <  1:12703/140‑1 (MQ=255)             cgcgGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCa                                                                                                                          >  1:210039/1‑140 (MQ=255)             cgcgGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCa                                                                                                                          >  2:12703/1‑140 (MQ=255)                gggCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCg                                                                                                                       <  1:316894/140‑1 (MQ=255)                    acgcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCa                                                                                                                   <  2:498221/140‑1 (MQ=255)                          aaCGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCa                                                                                                             <  2:713669/140‑1 (MQ=255)                                gccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCa                                                                                                       >  1:448313/1‑140 (MQ=255)                                    aTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGa                                                                                                                 <  2:126421/126‑1 (MQ=255)                                    aTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGa                                                                                                                 >  1:126421/1‑126 (MQ=255)                                       tCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACg                                                                                                <  1:935545/140‑1 (MQ=255)                                                 ccACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCA‑‑‑‑‑GGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCt                                                                                 >  1:839885/1‑140 (MQ=255)                                                  cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGa                                                                                     <  2:546126/140‑1 (MQ=255)                                                            ttATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGagag                                                                           >  1:610318/1‑140 (MQ=255)                                                             tATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg                                                                          <  2:100508/140‑1 (MQ=255)                                                             tATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg                                                                          <  2:610318/140‑1 (MQ=255)                                                               tCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg                                                                          >  1:30698/1‑138 (MQ=255)                                                               tCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg                                                                          <  2:30698/138‑1 (MQ=255)                                                               tCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAC‑TTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAg                                                                       >  2:94470/1‑140 (MQ=255)                                                                 cAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGt                                                                      <  2:137559/140‑1 (MQ=255)                                                                     ttCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAC‑TTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCa                                                                 <  1:94470/140‑1 (MQ=255)                                                                        aGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAAc                                                               <  1:527187/140‑1 (MQ=255)                                                                         gCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAAcc                                                              >  1:269976/1‑140 (MQ=255)                                                                         gCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAAcc                                                              >  2:601750/1‑140 (MQ=255)                                                                         gCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAAcc                                                              >  1:480119/1‑140 (MQ=255)                                                                             cagcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACtt                                                          <  2:736424/140‑1 (MQ=255)                                                                                  tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                     <  2:269976/140‑1 (MQ=255)                                                                                  tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                     >  2:592153/1‑140 (MQ=255)                                                                                    tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGt                                                   >  1:602685/1‑140 (MQ=255)                                                                                              ggATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAt                                                <  2:397435/133‑1 (MQ=255)                                                                                              ggATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAt                                                >  1:397435/1‑133 (MQ=255)                                                                                              ggATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTaca                                         <  2:448313/140‑1 (MQ=255)                                                                                              ggATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTaca                                         >  1:882832/1‑140 (MQ=255)                                                                                              ggATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTa                                           <  1:290895/138‑1 (MQ=255)                                                                                              ggATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTa                                           >  2:290895/1‑138 (MQ=255)                                                                                               gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTacac                                        >  2:115440/1‑140 (MQ=255)                                                                                               gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGGCAGGACACGGCCAACACCGACGGTGGGGGGGAAGGCCGCAACCACTTTCGCCGTGATTGGTacac                                        >  2:916677/1‑140 (MQ=255)                                                                                                         ccAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACg                              >  2:90494/1‑140 (MQ=255)                                                                                                          cAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACgg                             >  1:508228/1‑140 (MQ=255)                                                                                                                cGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGa                       <  1:115440/140‑1 (MQ=255)                                                                                                                cGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGa                       <  2:210039/140‑1 (MQ=255)                                                                                                                cGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGa                       <  1:927842/140‑1 (MQ=255)                                                                                                                   tATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACgg                    >  2:37482/1‑140 (MQ=255)                                                                                                                      cAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGTAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGa                                 <  2:111954/124‑1 (MQ=255)                                                                                                                      cAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGTAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGa                                 >  1:111954/1‑124 (MQ=255)                                                                                                                         ccGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTgg              <  2:321397/140‑1 (MQ=255)                                                                                                                         ccGGAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTgg              <  1:607159/140‑1 (MQ=255)                                                                                                                           ggAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGa                       >  2:242137/1‑129 (MQ=255)                                                                                                                           ggAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGa                       >  2:599155/1‑129 (MQ=255)                                                                                                                           ggAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGa                       <  1:599155/129‑1 (MQ=255)                                                                                                                           ggAACGTCGAAGAAGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGa                       <  1:242137/129‑1 (MQ=255)                                                                                                                              aCGTCGAAGAAGCCCTGAATCTGTTCAGCGAGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcgcgc         <  2:602685/140‑1 (MQ=255)                                                                                                                                   gaagaaGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGc    <  1:768665/140‑1 (MQ=255)                                                                                                                                   gaagaaGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGc    <  2:839885/140‑1 (MQ=255)                                                                                                                                     agaaGCCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCa  <  2:508228/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCA  >  NC_000913/1261329‑1261600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑