Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,927	T→C	R236R (CGT→CGC)	yciV →	RNA/ssDNA 5'‑>3' exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,927	0	T	C	100.0%	165.3 / NA	48	R236R (CGT→CGC)	yciV	RNA/ssDNA 5'‑>3' exonuclease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/28);  total (20/28)

CGGTAAGGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCA  >  NC_000913/1323792‑1324058                                                                                                                                        |                                                                                                                                    cGGTAAGGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCAccc                                                                                                                                 <  2:217524/140‑1 (MQ=255)                     aTCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGt                                                                                                             <  2:394301/140‑1 (MQ=255)                      tCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTc                                                                                                            >  1:540801/1‑140 (MQ=255)                        cTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAg                                                                                                          <  2:134547/140‑1 (MQ=255)                         tGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGc                                                                                                         >  1:441026/1‑140 (MQ=255)                          gggCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGca                                                                                                        <  2:441026/140‑1 (MQ=255)                           ggCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGcat                                                                                                       >  2:809270/1‑140 (MQ=255)                               gTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTgcg                                                                                                                >  1:199899/1‑127 (MQ=255)                               gTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTgcg                                                                                                                <  2:199899/127‑1 (MQ=255)                               gTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCAtt                                                                                                   >  2:322361/1‑140 (MQ=255)                                tACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCAttt                                                                                                  >  1:188482/1‑140 (MQ=255)                                     tCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAg                                                                                                          >  1:355488/1‑127 (MQ=255)                                     tCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAg                                                                                                          <  2:355488/127‑1 (MQ=255)                                     tCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATggg                                                                                             <  1:479737/140‑1 (MQ=255)                                     tCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATggg                                                                                             <  1:731621/140‑1 (MQ=255)                                       ttAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCa                                                                                           <  2:540801/140‑1 (MQ=255)                                       ttAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCa                                                                                           <  1:765866/140‑1 (MQ=255)                                        tAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCAt                                                                                          >  1:257907/1‑140 (MQ=255)                                          gTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATggg                                                                                             >  1:143222/1‑135 (MQ=255)                                          gTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATggg                                                                                             <  2:143222/135‑1 (MQ=255)                                          gTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATca                                                                                        >  2:548051/1‑140 (MQ=255)                                          gTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATca                                                                                        >  2:467726/1‑140 (MQ=255)                                            gCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATcaca                                                                                      <  2:188482/140‑1 (MQ=255)                                               aaaTGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATggg                                                                                             >  2:367833/1‑130 (MQ=255)                                               aaaTGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATggg                                                                                             <  1:367833/130‑1 (MQ=255)                                                  tGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATc                                                                                <  2:246567/140‑1 (MQ=255)                                                     cTGAAAAGATTGGTTGCGCATTTTGCCGAACACCATGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGAt                                                                                 <  1:271116/136‑1 (MQ=255)                                                     cTGAAAAGATTGGTTGCGCATTTTGCCGAACACCATGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGAt                                                                                 >  2:271116/1‑136 (MQ=255)                                                     cTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGa                                                                             >  1:818468/1‑140 (MQ=255)                                                       gAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGAtt                                                                           >  2:451512/1‑140 (MQ=255)                                                           aGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTtca                                                                       <  2:823876/140‑1 (MQ=255)                                                                  ttGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCa                                                                <  1:612591/140‑1 (MQ=255)                                                                  ttGCACATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCa                                                                <  1:793929/140‑1 (MQ=255)                                                                       cATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGccc                                                           <  1:81884/140‑1 (MQ=255)                                                                                 aaCACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGaa                                                 <  1:322361/140‑1 (MQ=255)                                                                                 aaCACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGaa                                                 >  1:453436/1‑140 (MQ=255)                                                                                      cacGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGaa                                                 >  2:473047/1‑135 (MQ=255)                                                                                      cacGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGaa                                                 <  1:473047/135‑1 (MQ=255)                                                                                              cgcgATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAAc                                    >  2:801188/1‑140 (MQ=255)                                                                                                           gcgcAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGc                       <  2:453436/140‑1 (MQ=255)                                                                                                            cgcAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCa                      <  1:548051/140‑1 (MQ=255)                                                                                                            cgcAGTGCCAGCAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCa                      <  1:467726/140‑1 (MQ=255)                                                                                                                       cAATCGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAgg           <  1:801188/140‑1 (MQ=255)                                                                                                                           cGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTc       >  2:502114/1‑140 (MQ=255)                                                                                                                           cGCCCAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATCTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTc       >  1:253856/1‑140 (MQ=255)                                                                                                                              ccAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTgg    <  1:451512/140‑1 (MQ=255)                                                                                                                              ccAATGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTgg    <  2:609109/140‑1 (MQ=255)                                                                                                                                aaTGAACGCACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATCTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCa  <  2:253856/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CGGTAAGGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGTTGAAGGCGTCTGGCA  >  NC_000913/1323792‑1324058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑