| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,466,533 | T→C | pseudogene (1142/2518 nt) | ydbA → | putative outer membrane protein, N‑terminal fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,466,533 | 0 | T | C | 94.6% | 107.1 / ‑1.6 | 37 | pseudogene (1142/2518 nt) | ydbA | putative outer membrane protein, N‑terminal fragment |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (19/16); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
GATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAAC > NC_000913/1466397‑1466666 | gATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCAc < 2:590463/140‑1 (MQ=255) gTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAg > 1:246617/1‑140 (MQ=255) aTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCg < 2:563793/129‑1 (MQ=255) aTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCg > 1:563793/1‑129 (MQ=255) aaCGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTAt > 1:344814/1‑140 (MQ=255) taataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAgg < 2:478672/140‑1 (MQ=255) ataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGt > 2:931647/1‑131 (MQ=255) ataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGt < 1:931647/131‑1 (MQ=37) aCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGAc < 2:642676/140‑1 (MQ=255) tATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAgg < 2:262596/140‑1 (MQ=18) ttCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCg > 1:844781/1‑140 (MQ=18) tCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCgg < 1:313313/140‑1 (MQ=21) tCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCgg < 2:246617/140‑1 (MQ=21) cAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGctgt < 2:344814/140‑4 (MQ=18) gACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgttgt > 1:923228/1‑140 (MQ=37) ggCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaa < 1:381807/140‑1 (MQ=255) cGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaaca > 1:715160/1‑140 (MQ=255) tGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGaa > 2:716215/1‑140 (MQ=255) tGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaacaac < 1:721763/133‑1 (MQ=255) tGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaacaac > 2:721763/1‑133 (MQ=255) cggcggcggTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAgcg < 2:445337/140‑1 (MQ=255) gcggcggTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGcc < 2:923228/140‑1 (MQ=255) aTTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTgg < 1:716215/140‑1 (MQ=255) ccGGCGACAGCGCAACGGGGGATAGCAAGGAGCCTCA‑GACGGCTATAAAACAGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACg < 2:670739/140‑1 (MQ=18) cGGCGACAGCGCAACGGTGGATAACAATG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGc > 2:512199/1‑140 (MQ=255) cAACGGTGGATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGt > 2:116973/1‑140 (MQ=255) gATAACAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGc > 2:940218/1‑140 (MQ=255) aaCAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCAc > 2:883480/1‑140 (MQ=255) aCAAGG‑GCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACg < 1:116973/140‑1 (MQ=255) gg‑gCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCGGATTAACGGTGATGACGCCACGGCaa > 2:3310/1‑140 (MQ=255) gg‑gCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCa > 2:601390/1‑134 (MQ=255) gg‑gCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCa < 1:601390/134‑1 (MQ=255) gCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaac > 2:733043/1‑140 (MQ=255) gCACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaac > 1:73373/1‑140 (MQ=255) cACCATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaaca > 1:651708/1‑140 (MQ=255) cATGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaaca < 2:844781/140‑1 (MQ=255) tGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaacaac > 1:559281/1‑140 (MQ=255) tGACCGCCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaacaac > 1:50020/1‑140 (MQ=255) | GATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGG‑GCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAAC > NC_000913/1466397‑1466666 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |