Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,610,001	A→T	L227Q (CTG→CAG)	uxaB ←	tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,610,001	0	A	T	100.0%	156.6 / NA	45	L227Q (CTG→CAG)	uxaB	tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/22);  total (23/22)

TCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCA  >  NC_000913/1609866‑1610127                                                                                                                                        |                                                                                                                               tCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGttt                                                                                                                            >  2:931684/1‑140 (MQ=255) tCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGttt                                                                                                                            >  2:318437/1‑140 (MQ=255) tCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGttt                                                                                                                            >  1:349796/1‑140 (MQ=255) tCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGttt                                                                                                                            >  1:328257/1‑140 (MQ=255)        tCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACt                                                                                                                     >  1:598050/1‑140 (MQ=255)            cACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCAt                                                                                                                 >  2:819548/1‑140 (MQ=255)            cACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCAt                                                                                                                 >  2:280755/1‑140 (MQ=255)              cGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATcg                                                                                                               <  1:280755/140‑1 (MQ=255)                         tatTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAAcc                                                                                                    <  1:177405/140‑1 (MQ=255)                         tatTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAAcc                                                                                                    <  1:303888/140‑1 (MQ=255)                             tGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTa                                                                                                >  2:868421/1‑140 (MQ=255)                              gTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTaa                                                                                               <  2:964480/140‑1 (MQ=255)                                  aGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCg                                                                                                   <  1:253810/132‑1 (MQ=255)                                  aGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCg                                                                                                   >  2:253810/1‑132 (MQ=255)                                  aGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGAt                                                                                           >  2:874372/1‑140 (MQ=255)                                           tCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTccac                                                                                  <  1:819548/140‑1 (MQ=255)                                           tCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTccac                                                                                  <  1:931684/140‑1 (MQ=255)                                                  cTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATa                                                                                          >  2:760091/1‑125 (MQ=255)                                                  cTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATa                                                                                          <  1:760091/125‑1 (MQ=255)                                                    aaGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGa                                                                         >  1:483887/1‑140 (MQ=255)                                                    aaGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGa                                                                         >  2:189674/1‑140 (MQ=255)                                                         tttCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGa                                                                    <  1:189674/140‑1 (MQ=255)                                                         tttCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGa                                                                    <  1:715597/140‑1 (MQ=255)                                                            cGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTccac                                                                                  <  1:14032/123‑1 (MQ=255)                                                            cGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTccac                                                                                  >  2:14032/1‑123 (MQ=255)                                                             ggTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCg                                                                             <  2:267021/127‑1 (MQ=255)                                                             ggTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCg                                                                             >  1:267021/1‑127 (MQ=255)                                                               tCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGt                                                              >  2:518390/1‑140 (MQ=255)                                                                 ccTGAATCACAAAAAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAAACGGTAACGAAACGGTCCACCAGCGTAGAACAGAAGCTGTTa                                                            >  2:484748/1‑140 (MQ=255)                                                                   tGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGc                                                          <  2:598050/140‑1 (MQ=255)                                                                   tGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGc                                                          <  2:607921/140‑1 (MQ=255)                                                                               aGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCa                                              <  1:320491/140‑1 (MQ=255)                                                                                ggTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCTCTTCATCGCGCGGATAACCGGTAACGATACGATCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCAt                                             <  1:484748/140‑1 (MQ=255)                                                                                 gTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCAt                                             >  1:294236/1‑139 (MQ=255)                                                                                 gTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCAt                                             <  2:294236/139‑1 (MQ=255)                                                                                     aaGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAAt                                        >  1:595667/1‑140 (MQ=255)                                                                                       gtgtTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATaa                                      <  2:401561/140‑1 (MQ=255)                                                                                                 gtgtCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTgg                            <  1:275144/140‑1 (MQ=255)                                                                                                  tgtCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGc                           <  1:318437/140‑1 (MQ=255)                                                                                                  tgtCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGc                           <  1:276570/140‑1 (MQ=255)                                                                                                  tgtCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGc                           >  2:276570/1‑140 (MQ=255)                                                                                                            aCCATCGTGATAACCCAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCAct                 >  1:631236/1‑140 (MQ=255)                                                                                                                           cAGTTCTTCTTCCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCgca  >  1:275475/1‑140 (MQ=255)                                                                                                                                   cttcCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCAt        <  1:236812/126‑1 (MQ=255)                                                                                                                                   cttcCTGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCAt        >  2:236812/1‑126 (MQ=255)                                                                                                                                        |                                                                                                                               TCAACAATCAGCACGTTGAGCGGATATTTGTCCAGACGCAGTTCAGTCGCTAAGGATTTCGGTCCCTGAATCACAAACAGGTAAAAGTGTTCAGCGGTGTCGAGAAAACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCA  >  NC_000913/1609866‑1610127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑