Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,675,569	G→A	N264N (AAC→AAT)	pntB ←	pyridine nucleotide transhydrogenase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,675,569	0	G	A	100.0%	237.8 / NA	67	N264N (AAC→AAT)	pntB	pyridine nucleotide transhydrogenase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/34);  total (33/34)

CACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTT  >  NC_000913/1675434‑1675697                                                                                                                                        |                                                                                                                                 cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCa                                                                                                                              <  1:970807/140‑1 (MQ=255) cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCa                                                                                                                              <  2:373909/140‑1 (MQ=255) cACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTGCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCa                                                                                                                              >  2:583523/1‑140 (MQ=255)    tGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCg                                                                                                                           >  2:194214/1‑140 (MQ=255)    tGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCg                                                                                                                           >  2:272630/1‑140 (MQ=255)      aaTGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGcc                                                                                                                         <  1:272630/140‑1 (MQ=255)       aTGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCt                                                                                                                        <  2:102030/140‑1 (MQ=255)        tGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCtt                                                                                                                       <  1:711101/140‑1 (MQ=255)        tGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCtt                                                                                                                       <  1:693536/140‑1 (MQ=255)           gAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTaca                                                                                                                    <  1:179029/140‑1 (MQ=255)           gAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTaca                                                                                                                    >  2:449937/1‑140 (MQ=255)           gAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTaca                                                                                                                    >  1:148089/1‑140 (MQ=255)           gAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTaca                                                                                                                    <  2:148089/140‑1 (MQ=255)             gTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTAcaca                                                                                                                  <  1:117467/140‑1 (MQ=255)             gTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTAcaca                                                                                                                  <  2:457234/140‑1 (MQ=255)             gTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTAcaca                                                                                                                  <  1:765876/140‑1 (MQ=255)             gTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTAcaca                                                                                                                  <  1:560913/140‑1 (MQ=255)             gTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTAcaca                                                                                                                  <  1:396304/140‑1 (MQ=255)             gTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCACCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTAcaca                                                                                                                  <  2:810862/140‑1 (MQ=255)                          tCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAg                                                                                                     >  1:429175/1‑140 (MQ=255)                                 tctcTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATa                                                                                                                >  1:546893/1‑122 (MQ=255)                                 tctcTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATa                                                                                                                <  2:546893/122‑1 (MQ=255)                                      tctGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCga                                                                                         >  2:553412/1‑140 (MQ=255)                                       ctGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCgaa                                                                                        >  2:489195/1‑140 (MQ=255)                                       ctGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCgaa                                                                                        <  1:489195/140‑1 (MQ=255)                                          cGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCgaagaa                                                                                     >  1:980667/1‑140 (MQ=255)                                            gTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAAcc                                                                                   <  2:368493/140‑1 (MQ=255)                                               aTTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGc                                                                                               <  2:935599/125‑1 (MQ=255)                                               aTTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGc                                                                                               >  1:935599/1‑125 (MQ=255)                                                  tCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTAATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAg                                                                             <  2:417199/140‑1 (MQ=255)                                                      ggTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCa                                                                         <  1:194214/140‑1 (MQ=255)                                                      ggTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCa                                                                         <  1:364145/140‑1 (MQ=255)                                                       gTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGAc                                                                                <  1:311056/132‑1 (MQ=255)                                                       gTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGAc                                                                                >  2:311056/1‑132 (MQ=255)                                                       gTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCAc                                                                        <  2:192103/140‑1 (MQ=255)                                                                 aCTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAAt                                                              <  2:742727/140‑1 (MQ=255)                                                                  cTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATc                                                             >  1:915463/1‑140 (MQ=255)                                                                  cTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATc                                                             >  2:78986/1‑140 (MQ=255)                                                                         atcatcGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGt                                                      <  1:167077/140‑1 (MQ=255)                                                                           catcGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCg                                                    >  2:470963/1‑140 (MQ=255)                                                                           catcGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCg                                                    >  2:39273/1‑140 (MQ=255)                                                                            atcGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGt                                                   >  2:117940/1‑140 (MQ=255)                                                                             tcGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGtt                                                  >  1:984640/1‑140 (MQ=255)                                                                                ccAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCt                                               >  1:187649/1‑140 (MQ=255)                                                                                 cAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTg                                              <  2:187649/140‑1 (MQ=255)                                                                                 cAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTg                                              <  1:583523/140‑1 (MQ=255)                                                                                    tAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGc                                           >  2:341274/1‑140 (MQ=255)                                                                                        agagCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATaa                                       >  1:274573/1‑140 (MQ=255)                                                                                          agCCGTCGGTGCCGAAACCGCCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAg                                     >  2:825727/1‑140 (MQ=255)                                                                                          agCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAg                                     >  2:278962/1‑140 (MQ=255)                                                                                           gCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGc                                    >  2:592235/1‑140 (MQ=255)                                                                                            ccGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGcc                                   >  1:125264/1‑140 (MQ=255)                                                                                              gTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCccg                                 >  1:111807/1‑140 (MQ=255)                                                                                              gTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCccg                                 >  1:459407/1‑140 (MQ=255)                                                                                                    gCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCccgcagccg                           >  1:682401/1‑140 (MQ=255)                                                                                                    gCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCccgcagccg                           >  1:78185/1‑140 (MQ=255)                                                                                                    gCCGAAACCACCCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCccgcagccg                           >  2:632407/1‑140 (MQ=255)                                                                                                            caccCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAgccgcc                   <  2:111807/140‑1 (MQ=255)                                                                                                             accCGCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGccc                  <  1:592235/140‑1 (MQ=255)                                                                                                                 gCAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGcc              <  2:984640/140‑1 (MQ=255)                                                                                                                  cAATAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCg             >  1:516617/1‑140 (MQ=255)                                                                                                                   aaTAACGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCgg            <  1:637693/140‑1 (MQ=255)                                                                                                                      aaCGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGt         <  1:78986/140‑1 (MQ=255)                                                                                                                        cGCTGATAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTAc       <  2:516617/140‑1 (MQ=255)                                                                                                                             aTAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGtt  >  2:289284/1‑140 (MQ=255)                                                                                                                             aTAAAGGAACGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGtt  >  2:204441/1‑140 (MQ=255)                                                                                                                                    aaCGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGtt  >  2:543534/1‑133 (MQ=255)                                                                                                                                    aaCGATTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGtt  <  1:543534/133‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 CACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCGCGGTGCTCACCCACTTCCTGATCATCGCCAGTAGAAGAGCCGTCGGTGCCGAAACCACCCGCAATAACGCTGATAAAGGAACGGTTCATCGCCTTACACATAATGTAAGAAAGGATAGCCCCCGAAGAACCGACCAGCGCACCGGTCACAATCAGCAGGTCGTTGCTGAGCATAAAGCCCGCAGCCGCAGCCGCCCAGCCGGAGTACGAGTT  >  NC_000913/1675434‑1675697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑