Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,696,946	G→A	A36V (GCC→GTC)	uidR ←	DNA‑binding transcriptional repressor UidR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,696,946	0	G	A	100.0%	213.4 / NA	61	A36V (GCC→GTC)	uidR	DNA‑binding transcriptional repressor UidR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/32);  total (29/32)

ATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTAAACCATTATAGTTAGCGTGAATAA  >  NC_000913/1696811‑1697078                                                                                                                                        |                                                                                                                                     aTCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGTAAGATTTACAGATGACttt                                                                                                                                  <  2:575768/140‑1 (MQ=255) aTCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACttt                                                                                                                                  <  1:642615/140‑1 (MQ=255)      ttCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCg                                                                                                                             <  1:862577/140‑1 (MQ=255)               ttCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACtgt                                                                                                                    <  1:224769/140‑1 (MQ=255)                     gAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCAt                                                                                                                               >  2:434249/1‑123 (MQ=255)                     gAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCAt                                                                                                                               <  1:434249/123‑1 (MQ=255)                         cGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCAtt                                                                                                          >  1:873642/1‑140 (MQ=255)                         cGGGCAAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCAtt                                                                                                          >  1:158131/1‑140 (MQ=255)                               aGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGa                                                                                                    <  1:536935/140‑1 (MQ=255)                                        tcCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGAGTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAAtctc                                                                                           <  2:786374/140‑1 (MQ=255)                                             gTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCTCTGTGAAATCCATTTTCTGAAAAAATCTCTCTgg                                                                                      <  2:873642/140‑1 (MQ=255)                                                 tGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGgcagc                                                                                  <  1:34101/140‑1 (MQ=255)                                                  gTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGgcagca                                                                                 >  2:552944/1‑140 (MQ=255)                                                  gTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGgcagca                                                                                 >  2:34968/1‑140 (MQ=255)                                                  gTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGgcagca                                                                                 >  1:615952/1‑140 (MQ=255)                                                  gTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGgca                                                                                    <  2:591287/137‑1 (MQ=255)                                                  gTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGgca                                                                                    >  1:591287/1‑137 (MQ=255)                                                           tCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATc                                                                        <  1:552944/140‑1 (MQ=255)                                                             gCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCg                                                                      >  2:583774/1‑140 (MQ=255)                                                               cTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATc                                                                        <  1:263780/136‑1 (MQ=255)                                                               cTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATc                                                                        >  2:263780/1‑136 (MQ=255)                                                               cTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCggg                                                                    <  1:943212/140‑1 (MQ=255)                                                                tGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGt                                                                   >  2:490800/1‑140 (MQ=255)                                                                 gAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTc                                                                  >  1:182442/1‑140 (MQ=255)                                                                      aaGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCgtgt                                                             <  2:655177/140‑1 (MQ=255)                                                                            tcttTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTtg                                                       >  1:344522/1‑140 (MQ=255)                                                                              tttGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTtgtg                                                     >  2:590689/1‑140 (MQ=255)                                                                                tGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCt                                                   <  1:313280/140‑1 (MQ=255)                                                                                 ggAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCtt                                                  <  1:490800/140‑1 (MQ=255)                                                                                   agaTGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCtt                                                  <  2:589026/138‑1 (MQ=255)                                                                                   agaTGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCtt                                                  >  1:589026/1‑138 (MQ=255)                                                                                       gAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCt                                            >  1:443227/1‑140 (MQ=255)                                                                                            ggTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTGCGTGTCGGTTGTGCCTCAGTCTGCATg                                       <  2:615952/140‑1 (MQ=255)                                                                                            ggTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATg                                       <  2:182442/140‑1 (MQ=255)                                                                                             gTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCa                                         <  2:658985/137‑1 (MQ=255)                                                                                             gTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCa                                         >  1:658985/1‑137 (MQ=255)                                                                                                  agagCGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATcc                                 <  1:590689/140‑1 (MQ=255)                                                                                                      cGTCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCcatca                             >  2:370893/1‑140 (MQ=255)                                                                                                        tCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTc                                             >  1:296318/1‑122 (MQ=255)                                                                                                        tCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTc                                             <  2:296318/122‑1 (MQ=255)                                                                                                        tCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATc                                  >  1:352561/1‑133 (MQ=255)                                                                                                        tCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATc                                  <  2:352561/133‑1 (MQ=255)                                                                                                        tCCCGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCAtt                           >  1:240569/1‑140 (MQ=255)                                                                                                         cccGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTa                          >  1:762230/1‑140 (MQ=255)                                                                                                         cccGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTa                          <  1:522301/140‑1 (MQ=255)                                                                                                         cccGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTa                          <  1:547472/140‑1 (MQ=255)                                                                                                         cccGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTa                          <  1:583774/140‑1 (MQ=255)                                                                                                           cGGGACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTaaa                        <  1:271632/140‑1 (MQ=255)                                                                                                              gACTAATGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTAAACCa                     >  2:547702/1‑140 (MQ=255)                                                                                                                   aTGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGTATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTaaa                        <  1:521717/132‑1 (MQ=255)                                                                                                                   aTGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGTATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTaaa                        >  2:521717/1‑132 (MQ=255)                                                                                                                   aTGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTaaa                        <  2:116397/132‑1 (MQ=255)                                                                                                                   aTGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTaaa                        >  1:116397/1‑132 (MQ=255)                                                                                                                   aTGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTa                          >  1:915437/1‑130 (MQ=255)                                                                                                                   aTGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTa                          <  2:915437/130‑1 (MQ=255)                                                                                                                   aTGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTAAAcc                      >  1:877461/1‑134 (MQ=255)                                                                                                                   aTGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTAAACCATtata                >  2:399497/1‑140 (MQ=255)                                                                                                                   aTGGCGCAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTAAACCATtata                >  2:767217/1‑140 (MQ=255)                                                                                                                   aTGGCGCAAGACTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCAGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTAAAcc                      <  2:877461/134‑1 (MQ=255)                                                                                                                         cAAGATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTAAACCATTATAGTTAGc          >  1:421922/1‑140 (MQ=255)                                                                                                                            gATTTACAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTAAACCATTATAGTTAGCGTg       <  2:240569/140‑1 (MQ=255)                                                                                                                                 aCAGATGACTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTAAACCATTATAGTTAGCGTGAATaa  <  1:34968/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     ATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGATCCGGGTCCGTGTCGGTTGTGCTTCAGTCTGCATGTTATCCATCATTAAACCATTATAGTTAGCGTGAATAA  >  NC_000913/1696811‑1697078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑