Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,853,128	T→C	I284M (ATA→ATG)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,853,128	0	T	C	100.0%	139.6 / NA	39	I284M (ATA→ATG)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/20);  total (19/20)

AGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTC  >  NC_000913/1852992‑1853263                                                                                                                                         |                                                                                                                                        aGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATc                                                                                                                                      >  2:398739/1‑140 (MQ=255) aGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATc                                                                                                                                      >  1:712788/1‑140 (MQ=255)     aaaTAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATa                                                                                                                                  <  2:92385/140‑1 (MQ=255)      aaTAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATAc                                                                                                                                 <  1:644896/140‑1 (MQ=255)                                aattaatGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCa                                                                                                       >  1:627766/1‑140 (MQ=255)                                 attaatGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCAt                                                                                                      <  2:974339/140‑1 (MQ=255)                                  ttaatGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCAtt                                                                                                     <  2:712788/140‑1 (MQ=255)                                                    ttCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATc                                                                                   <  1:47254/140‑1 (MQ=255)                                                       cTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATcgcg                                                                                >  1:148174/1‑140 (MQ=255)                                                        tACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGa                                                                               <  1:398739/140‑1 (MQ=255)                                                         aCCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGAc                                                                              <  1:547769/140‑1 (MQ=255)                                                              aGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATg                                                                         >  1:725173/1‑140 (MQ=255)                                                                cgcCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTa                                                                       <  1:884165/140‑1 (MQ=255)                                                                      tCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTAcgtcgt                                                                 <  2:725173/140‑1 (MQ=255)                                                                             aaCAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACAttt                                                          <  2:148174/140‑1 (MQ=255)                                                                               cAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTa                                                                       >  1:270303/1‑125 (MQ=255)                                                                               cAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTa                                                                       <  2:270303/125‑1 (MQ=255)                                                                                    gTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCttt                                                   >  2:727520/1‑140 (MQ=255)                                                                                    gTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCttt                                                   >  2:570063/1‑140 (MQ=255)                                                                                    gTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCttt                                                   >  1:612098/1‑140 (MQ=255)                                                                                    gTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCttt                                                   >  1:623279/1‑140 (MQ=255)                                                                                       aTTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTaaa                                                >  2:729237/1‑140 (MQ=255)                                                                                       aTTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTaaa                                                >  1:564555/1‑140 (MQ=255)                                                                                        ttAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAAc                                               <  2:612098/140‑1 (MQ=255)                                                                                         tAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACa                                              <  2:623279/140‑1 (MQ=255)                                                                                               tGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGcc                                        <  1:727520/140‑1 (MQ=255)                                                                                               tGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGcc                                        >  1:515727/1‑140 (MQ=255)                                                                                                  tttATCGACATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGa                                     <  1:802666/140‑1 (MQ=255)                                                                                                         aCATCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTAcc                              <  2:564555/140‑1 (MQ=255)                                                                                                           aTCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAg                                  <  1:497809/134‑1 (MQ=255)                                                                                                           aTCAATGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAg                                  >  2:497809/1‑134 (MQ=255)                                                                                                                tGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGAtt                       >  2:478330/1‑140 (MQ=255)                                                                                                                tGCCGGAGTTAACAAATATGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGAtt                       >  1:672518/1‑140 (MQ=255)                                                                                                                                tatGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGt       <  2:627766/140‑1 (MQ=255)                                                                                                                                  tGGTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTg     >  1:942923/1‑140 (MQ=255)                                                                                                                                    gTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGct   <  2:672518/140‑1 (MQ=255)                                                                                                                                    gTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGct   >  1:878594/1‑140 (MQ=255)                                                                                                                                    gTCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGct   <  1:104416/140‑1 (MQ=255)                                                                                                                                     tCGGCATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAGTTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGctc  >  2:497352/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTC  >  NC_000913/1852992‑1853263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑