Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	272,657	T→C	intergenic (+3/‑190)	eyeA → / → ykfC	CP4‑6 prophage; small RNA EyeA/CP4‑6 prophage; PF00078 domain‑containing protein YkfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	272,657	0	T	C	100.0%	244.7 / NA	67	intergenic (+3/‑190)	eyeA/ykfC	CP4‑6 prophage; small RNA EyeA/CP4‑6 prophage; PF00078 domain‑containing protein YkfC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/33);  total (34/33)

AAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAG  >  NC_000913/272524‑272793                                                                                                                                      |                                                                                                                                         aaGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCt                                                                                                                                    <  1:186057/140‑1 (MQ=255) aaGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCt                                                                                                                                    <  2:940107/140‑1 (MQ=255)   gCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGa                                                                                                                                  >  2:257468/1‑140 (MQ=255)       tGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTcc                                                                                                                              >  2:930133/1‑140 (MQ=255)       tGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTcc                                                                                                                              >  1:339790/1‑140 (MQ=255)        gCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGcc                                                                                                                                     <  2:559805/132‑1 (MQ=255)        gCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGcc                                                                                                                                     >  1:559805/1‑132 (MQ=255)        gCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCca                                                                                                                             <  1:60287/140‑1 (MQ=255)         ccGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCcag                                                                                                                            >  2:507858/1‑140 (MQ=255)          cGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCcagg                                                                                                                           >  2:411149/1‑140 (MQ=255)          cGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCcagg                                                                                                                           <  2:720680/140‑1 (MQ=255)          cGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCcagg                                                                                                                           <  2:642311/140‑1 (MQ=255)            gTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCcaggca                                                                                                                         >  2:917939/1‑140 (MQ=255)             tCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCca                                                                                                                             <  2:539371/135‑1 (MQ=255)             tCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCca                                                                                                                             >  1:539371/1‑135 (MQ=255)              cGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCcaggcagg                                                                                                                       >  1:672748/1‑140 (MQ=255)                   gtgtTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAAc                                                                                                                  >  1:943358/1‑140 (MQ=255)                      ttttGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTaa                                                                                                                    <  1:968051/135‑1 (MQ=255)                      ttttGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTaa                                                                                                                    >  2:968051/1‑135 (MQ=255)                      ttttGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCgg                                                                                                               >  2:484058/1‑140 (MQ=255)                        ttGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCgggg                                                                                                             <  2:649225/140‑1 (MQ=255)                         tGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGa                                                                                                            <  2:339790/140‑1 (MQ=255)                              tGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCgggg                                                                                                             <  2:909528/134‑1 (MQ=255)                              tGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCgggg                                                                                                             >  1:909528/1‑134 (MQ=255)                                tATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAg                                                                                                     >  2:122119/1‑140 (MQ=255)                                   gCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGa                                                                                                  <  2:943358/140‑1 (MQ=255)                                     gAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACg                                                                                                <  1:507858/140‑1 (MQ=255)                                     gAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACg                                                                                                <  1:677126/140‑1 (MQ=255)                                          acCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGt                                                                                           >  1:126560/1‑140 (MQ=255)                                          acCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGt                                                                                           >  1:313187/1‑140 (MQ=255)                                            cGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTa                                                                                         >  1:367364/1‑140 (MQ=255)                                             ggggCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGCGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTaa                                                                                        <  2:672748/140‑1 (MQ=255)                                               ggCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAgg                                                                                      >  1:706239/1‑140 (MQ=255)                                                  agcagcGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAggggg                                                                                   <  1:930133/140‑1 (MQ=255)                                                   gcagcGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGc                                                                                  <  2:113269/140‑1 (MQ=255)                                                   gcagcGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGc                                                                                  <  2:367364/140‑1 (MQ=255)                                                    cagcGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCa                                                                                 <  2:126560/140‑1 (MQ=255)                                                    cagcGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCa                                                                                 <  2:706239/140‑1 (MQ=255)                                                    cagcGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCa                                                                                 <  2:313187/140‑1 (MQ=255)                                                      gcGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATg                                                                               >  1:412101/1‑140 (MQ=255)                                                              cACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTaa                                                                                        >  1:826682/1‑123 (MQ=255)                                                              cACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTaa                                                                                        <  2:826682/123‑1 (MQ=255)                                                              cACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGAt                                                                       >  2:708199/1‑140 (MQ=255)                                                                 gCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGAc                                                                    >  1:582059/1‑140 (MQ=255)                                                                   ttGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGAcgc                                                                  >  1:673120/1‑140 (MQ=255)                                                                    tGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGAcgcg                                                                 >  1:192270/1‑140 (MQ=255)                                                                    tGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGAcgcg                                                                 <  2:655784/140‑1 (MQ=255)                                                                              aCTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCg                                                       >  1:518511/1‑140 (MQ=255)                                                                                ttCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGa                                                     <  1:708199/140‑1 (MQ=255)                                                                                ttCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGa                                                     >  2:514225/1‑140 (MQ=255)                                                                                  cTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc                                                   <  2:673120/140‑1 (MQ=255)                                                                                   tGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCt                                                  >  1:733034/1‑140 (MQ=255)                                                                                     ccAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTc                                                <  2:728419/140‑1 (MQ=255)                                                                                            gCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCgtagta                                         <  1:379567/140‑1 (MQ=255)                                                                                                          tAAAGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa                           <  2:733034/140‑1 (MQ=255)                                                                                                            aaGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGaaa                         <  1:411149/140‑1 (MQ=255)                                                                                                            aaGAGTGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGaaa                         <  1:917939/140‑1 (MQ=255)                                                                                                                 tGCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCa                    <  1:122119/140‑1 (MQ=255)                                                                                                                  gCGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCAt                   >  2:386462/1‑140 (MQ=255)                                                                                                                   cGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCAtt                  >  1:956015/1‑140 (MQ=255)                                                                                                                   cGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCAtt                  >  2:952225/1‑140 (MQ=255)                                                                                                                   cGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCAtt                  >  1:327383/1‑140 (MQ=255)                                                                                                                   cGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCAtt                  >  1:948017/1‑140 (MQ=255)                                                                                                                   cGACGGGCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGACTCCGATGATGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCAtt                  >  2:882358/1‑140 (MQ=255)                                                                                                                         gCAGCCTCCTCAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATgg            >  2:176462/1‑140 (MQ=255)                                                                                                                                   cAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg  <  1:257468/140‑1 (MQ=255)                                                                                                                                   cAGCATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAg  <  2:192270/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         AAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAG  >  NC_000913/272524‑272793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑