Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,274,122	T→C	W587R (TGG→CGG)	yejA →	putative oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,274,122	0	T	C	100.0%	255.6 / NA	71	W587R (TGG→CGG)	yejA	putative oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/30);  total (41/30)

CACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGT  >  NC_000913/2273988‑2274258                                                                                                                                       |                                                                                                                                         cACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTAt                                                                                                                                     <  2:139884/140‑1 (MQ=255)      gATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGt                                                                                                                                >  1:188327/1‑140 (MQ=255)        tCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCa                                                                                                                              <  1:707078/140‑1 (MQ=255)           cgTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATa                                                                                                                           >  1:780749/1‑140 (MQ=255)                  aCGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGc                                                                                                                    >  2:709004/1‑140 (MQ=255)                     tGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCaa                                                                                                                 >  2:552016/1‑140 (MQ=255)                       gAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAAc                                                                                                               >  2:928151/1‑140 (MQ=255)                         attattACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTg                                                                                                             <  2:780749/140‑1 (MQ=255)                              taCATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTc                                                                                                        >  2:417237/1‑140 (MQ=255)                                  tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGcc                                                                                                    <  1:112405/140‑1 (MQ=255)                                  tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGcc                                                                                                    >  2:963641/1‑140 (MQ=255)                                  tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGcc                                                                                                    <  1:892431/140‑1 (MQ=255)                                  tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGcc                                                                                                    >  1:472880/1‑140 (MQ=255)                                    ctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAg                                                                                                  >  1:776929/1‑140 (MQ=255)                                     tgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTc                                                                                                        <  1:134107/133‑1 (MQ=255)                                     tgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTc                                                                                                        >  2:134107/1‑133 (MQ=255)                                     tgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGc                                                                                                 >  1:985790/1‑140 (MQ=255)                                      gcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCa                                                                                                <  1:417237/140‑1 (MQ=255)                                       ccAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCaa                                                                                               >  2:201989/1‑140 (MQ=255)                                         aaTGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAAc                                                                                             <  2:355082/140‑1 (MQ=255)                                              ggTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGga                                                                                        <  2:985790/140‑1 (MQ=255)                                                 aCATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGagag                                                                                     <  2:188327/140‑1 (MQ=255)                                                     ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGa                                                                                 >  2:249543/1‑140 (MQ=255)                                                     ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGa                                                                                 >  2:536287/1‑140 (MQ=255)                                                     ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGa                                                                                 >  1:622074/1‑140 (MQ=255)                                                     ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGa                                                                                 >  1:747584/1‑140 (MQ=255)                                                      gCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGAt                                                                                <  1:709004/140‑1 (MQ=255)                                                         gAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATggg                                                                             >  1:270853/1‑140 (MQ=255)                                                         gAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATggg                                                                             <  2:491353/140‑1 (MQ=255)                                                               cGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTa                                                                       >  2:174072/1‑140 (MQ=255)                                                                 tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTAcc                                                                     >  1:146869/1‑140 (MQ=255)                                                                      ccTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGAtt                                                                >  2:537/1‑140 (MQ=255)                                                                        tggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCg                                                              <  1:537/140‑1 (MQ=255)                                                                        tggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCg                                                              <  1:536287/140‑1 (MQ=255)                                                                           tggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCg                                                           >  1:735444/1‑140 (MQ=255)                                                                             ggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTc                                                         <  2:747584/140‑1 (MQ=255)                                                                             ggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGACACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTc                                                         <  1:916538/140‑1 (MQ=255)                                                                                tAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGt                                                      <  2:776929/140‑1 (MQ=255)                                                                                       tcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                               <  1:963641/140‑1 (MQ=255)                                                                                       tcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                               <  2:622074/140‑1 (MQ=255)                                                                                        cccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTg                                              >  1:318946/1‑140 (MQ=255)                                                                                        cccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTg                                              >  1:790144/1‑140 (MQ=255)                                                                                         ccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGa                                             >  2:854458/1‑140 (MQ=255)                                                                                             ccggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                         <  2:735444/140‑1 (MQ=255)                                                                                               ggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCaa                                       <  1:547074/140‑1 (MQ=255)                                                                                                gccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                               <  2:215590/131‑1 (MQ=255)                                                                                                gccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                               >  1:215590/1‑131 (MQ=255)                                                                                                 ccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACa                                     <  1:928151/140‑1 (MQ=255)                                                                                                  cgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                         >  2:815553/1‑135 (MQ=255)                                                                                                  cgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                         <  1:815553/135‑1 (MQ=255)                                                                                                    tGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTa                                  <  1:249543/140‑1 (MQ=255)                                                                                                        ccccATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                              <  2:270853/140‑1 (MQ=255)                                                                                                              cTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                        >  1:243565/1‑140 (MQ=255)                                                                                                                    ccTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                              <  1:894007/128‑1 (MQ=255)                                                                                                                    ccTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                              >  2:894007/1‑128 (MQ=255)                                                                                                                      tCGGTATCGATGCGTGGCGGTATGACGTCAATAAAGCGGCAAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                <  2:146869/140‑1 (MQ=255)                                                                                                                      tCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                <  2:243565/140‑1 (MQ=255)                                                                                                                      tCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                >  1:26209/1‑140 (MQ=255)                                                                                                                       cGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCa               >  2:704315/1‑140 (MQ=255)                                                                                                                        ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                        <  2:891490/130‑1 (MQ=255)                                                                                                                        ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                        >  1:891490/1‑130 (MQ=255)                                                                                                                        ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa              >  2:61208/1‑140 (MQ=255)                                                                                                                        ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa              >  2:425833/1‑140 (MQ=255)                                                                                                                        ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa              >  1:238414/1‑140 (MQ=255)                                                                                                                        ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa              >  1:717445/1‑140 (MQ=255)                                                                                                                        ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa              >  2:341044/1‑140 (MQ=255)                                                                                                                         gTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                        <  1:309696/129‑1 (MQ=255)                                                                                                                         gTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                        >  2:309696/1‑129 (MQ=255)                                                                                                                         gTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAc             >  2:638101/1‑140 (MQ=255)                                                                                                                         gTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAc             >  2:128276/1‑140 (MQ=255)                                                                                                                         gTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAc             >  2:399091/1‑140 (MQ=255)                                                                                                                                  ccTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATc    >  1:928840/1‑140 (MQ=255)                                                                                                                                    tggcggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGt  >  2:949129/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         CACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGT  >  NC_000913/2273988‑2274258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑