Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,296,418	T→C	T203A (ACC→GCC)	ccmB ←	heme trafficking system membrane protein CcmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,296,418	0	T	C	100.0%	143.1 / NA	43	T203A (ACC→GCC)	ccmB	heme trafficking system membrane protein CcmB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/24);  total (19/24)

GGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGTGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAG  >  NC_000913/2296290‑2296544                                                                                                                                 |                                                                                                                               ggATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCa                                                                                                                     >  2:943682/1‑140 (MQ=255)      gCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGcgcc                                                                                                                             <  1:215100/127‑1 (MQ=255)      gCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGcgcc                                                                                                                             >  2:215100/1‑127 (MQ=255)                gCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTg                                                                                                      <  2:713578/140‑1 (MQ=255)                gCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTg                                                                                                      >  1:806224/1‑140 (MQ=255)                     gtTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGTCAGCAACGCGCCTAAAATTGCCAGa                                                                                                 >  2:157486/1‑140 (MQ=255)                     gtTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGa                                                                                                 <  2:883902/140‑1 (MQ=255)                     gtTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGa                                                                                                 <  1:325879/140‑1 (MQ=255)                     gtTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGa                                                                                                 >  1:910658/1‑140 (MQ=255)                         tCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATAcc                                                                                             >  1:658747/1‑140 (MQ=255)                              aTAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCa                                                                                        >  2:325123/1‑140 (MQ=255)                                 gTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATCCCCGTCAACg                                                                                     <  2:265669/140‑1 (MQ=255)                                    tCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGc                                                                                  <  1:943682/140‑1 (MQ=255)                                     cGATACCAGACTCGACCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCTAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCa                                                                                 <  2:138656/140‑1 (MQ=255)                                      gATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaa                                                                                <  1:178356/140‑1 (MQ=255)                                      gATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaa                                                                                <  1:606430/140‑1 (MQ=255)                                       aTACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaaa                                                                               <  1:590422/140‑1 (MQ=255)                                                  gAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGc                                                                    >  2:941988/1‑140 (MQ=255)                                                  gAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGc                                                                    >  2:2986/1‑140 (MQ=255)                                                  gAACAAAAACCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGc                                                                    >  2:593117/1‑140 (MQ=255)                                                   aaCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaaa                                                                               <  1:70429/128‑1 (MQ=255)                                                   aaCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCaaa                                                                               >  2:70429/1‑128 (MQ=255)                                                   aaCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGcc                                                                   >  2:459686/1‑140 (MQ=255)                                                   aaCAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGcc                                                                   >  2:510648/1‑140 (MQ=255)                                                                   ccAGCGTTATTGAATGCTGATTCGTACCGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGcggcgg                                                   <  1:230938/140‑1 (MQ=255)                                                                        gTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCa                                              <  2:910658/140‑1 (MQ=255)                                                                           aTTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAg                                           <  1:325123/140‑1 (MQ=255)                                                                             tcaaTGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGAt                                         <  2:580603/138‑1 (MQ=255)                                                                             tGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGAt                                         <  1:510648/140‑1 (MQ=255)                                                                             tGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGAt                                         <  1:672413/140‑1 (MQ=255)                                                                                 tGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGt                                     >  1:979104/1‑140 (MQ=255)                                                                                 tGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGt                                     >  1:12871/1‑140 (MQ=255)                                                                                       ttCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATggg                               <  1:799436/140‑1 (MQ=255)                                                                                       ttCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCATCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATggg                               <  2:365451/140‑1 (MQ=255)                                                                                           tAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAg                           >  2:234191/1‑140 (MQ=255)                                                                                             aCGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTc                         <  2:979104/140‑1 (MQ=255)                                                                                              cGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCAGTGGCAAAGATGAGTAATGGGATAGTCa                        <  2:658747/140‑1 (MQ=255)                                                                                                   ccgccgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGc                      <  1:283640/137‑1 (MQ=255)                                                                                                   ccgccgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGc                      >  2:283640/1‑137 (MQ=255)                                                                                                       cgTCGCAAAAGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAaca               <  1:2986/140‑1 (MQ=255)                                                                                                             aaaaGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCgg                    <  2:723274/129‑1 (MQ=255)                                                                                                             aaaaGGACTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCgg                    >  1:723274/1‑129 (MQ=255)                                                                                                                    cTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGag  <  2:225649/140‑1 (MQ=255)                                                                                                                    cTTAATGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGag  >  1:912442/1‑140 (MQ=255)                                                                                                                         tGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGag  >  2:230802/1‑135 (MQ=255)                                                                                                                         tGTCGCGGCGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGag  <  1:230802/135‑1 (MQ=255)                                                                                                                                 |                                                                                                                               GGATCGCCAGTTGATGCAGTGTTTTCCACATAGTTTCGATACCAGACTCGAACAAAAATCAGTAATCCAGCGTTATTGAATGCTGATTCGTAACGCTGCCGCCGTCGCAAAAGGACTTAATGTCGCGGTGCCTGCCAGCAACGCGCCTAAAATTGCCAGATACCCGTCAACGGGCAAATGCATAGAAGCCGCGTCCATCGCGGCGGTGGCAAAGATGAGTAATGGGATAGTCAGCGGTAACACCAGTATGCTGAG  >  NC_000913/2296290‑2296544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑