Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,300,856	T→C	Y633C (TAC→TGC)	napA ←	periplasmic nitrate reductase subunit NapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,300,856	0	T	C	100.0%	166.8 / NA	48	Y633C (TAC→TGC)	napA	periplasmic nitrate reductase subunit NapA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/27);  total (21/27)

GTCGTTACCTTCGCTGTAACGCCACTGCGTTTCTTTACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCG  >  NC_000913/2300729‑2300985                                                                                                                                |                                                                                                                                  gTCGTTACCTTCGCTGTAACGCCACTGCGTTTCTTTACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACa                                                                                                                       <  2:411534/140‑1 (MQ=255)             gCTGTAACGCCACTGCGTTTCTTTACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCa                                                                                                           <  2:145872/140‑1 (MQ=255)              cTGTAACGCCACTGCGTTTCTTTACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAg                                                                                                          >  2:926420/1‑140 (MQ=255)                 tAACGCCACTGCGTTTCTTTACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATa                                                                                                       <  2:720534/140‑1 (MQ=255)                 tAACGCCACTGCGTTTCTTTACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGAACGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATa                                                                                                       >  2:863242/1‑140 (MQ=255)                         cTGCGTTTCTTTACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCa                                                                                               >  1:924643/1‑140 (MQ=255)                                  tttACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCggg                                                                                      <  2:711987/140‑1 (MQ=255)                                    tACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGAt                                                                                    >  2:670755/1‑140 (MQ=255)                                         ttCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATc                                                                               >  2:924964/1‑140 (MQ=255)                                           caccacCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTTACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGt                                                                             >  1:291838/1‑140 (MQ=255)                                           caccacCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCggg                                                                                      >  2:859504/1‑131 (MQ=255)                                           caccacCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCggg                                                                                      <  1:859504/131‑1 (MQ=255)                                           caccacCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGt                                                                             >  1:825306/1‑140 (MQ=255)                                              caccGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCa                                                                          >  1:318938/1‑140 (MQ=255)                                                 cGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCt                                                                       <  2:924643/140‑1 (MQ=255)                                                    ccAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGAt                                                                    <  1:304589/140‑1 (MQ=255)                                                        cgcAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCAAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTc                                                                >  1:58300/1‑140 (MQ=255)                                                             aCCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCa                                                           >  1:637913/1‑140 (MQ=255)                                                                    gcTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGa                                                    <  1:863242/140‑1 (MQ=255)                                                                    gcTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGa                                                    <  2:212502/140‑1 (MQ=255)                                                                              aGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAAt                                          <  1:926420/140‑1 (MQ=255)                                                                              aGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAAt                                          <  1:670755/140‑1 (MQ=255)                                                                                  aTCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGc                                      >  2:712027/1‑140 (MQ=255)                                                                                   tCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCt                                     <  1:924964/140‑1 (MQ=255)                                                                                   tCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCt                                     <  2:679321/140‑1 (MQ=255)                                                                                   tCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCt                                     <  2:637913/140‑1 (MQ=255)                                                                                    cGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTc                                    >  2:389033/1‑140 (MQ=255)                                                                                         ggTGCCAGATCGTGACCGTGACCGCGACCAACCCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATCCCGGGAATTTGCTCACTTc                               <  2:932559/140‑1 (MQ=255)                                                                                           tGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCg                             <  2:825306/140‑1 (MQ=255)                                                                                           tGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCg                             >  2:250558/1‑140 (MQ=255)                                                                                             ccAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGgt                           <  2:318938/140‑1 (MQ=255)                                                                                              cAGATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGgtg                          <  2:333936/140‑1 (MQ=255)                                                                                                gATCGTGACCGTGACCGCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGtgtg                        <  1:389033/140‑1 (MQ=255)                                                                                                     tgaccgtgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCt                                     <  2:523592/122‑1 (MQ=255)                                                                                                     tgaccgtgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCt                                     >  1:523592/1‑122 (MQ=255)                                                                                                     tgaccgtgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGgtg                          <  2:639622/133‑1 (MQ=255)                                                                                                     tgaccgtgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGgtg                          >  1:639622/1‑133 (MQ=255)                                                                                                     tgaccgtgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATa                   >  2:846507/1‑140 (MQ=255)                                                                                                     tgaccgtgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATa                   >  1:630076/1‑140 (MQ=255)                                                                                                     tgaccgtgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATa                   >  1:385327/1‑140 (MQ=255)                                                                                                       accgtgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACa                 <  2:384775/140‑1 (MQ=255)                                                                                                       accgtgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACa                 <  2:385327/140‑1 (MQ=255)                                                                                                           tgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGtgtg                        >  1:903311/1‑129 (MQ=255)                                                                                                           tgaccgCGACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGtgtg                        <  2:903311/129‑1 (MQ=255)                                                                                                           tgaccgCGACCAAACCATGCGCACTCTTAGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCAc             >  1:82722/1‑140 (MQ=255)                                                                                                                 cgACCAAACCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTa       <  2:630076/140‑1 (MQ=255)                                                                                                                      aaaCCATGCGCACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCg  <  1:712027/140‑1 (MQ=255)                                                                                                                      aaaCCATGCGCACTCTTAGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCg  <  2:82722/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                  GTCGTTACCTTCGCTGTAACGCCACTGCGTTTCTTTACCGTTCACCACCGGCCAGCGCAGACCGCGCGCTTTGTGGTAGTCATCGAACGGTGCCAGATCGTGACCGTGACCGCGACCAAACCATGCGTACTCTTCGAACAGCCCTTTTTGCAGATAGAAGCCCAGCTCGCGGGATTCATCGTTCAGCTGATCTTCCGCCAGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCG  >  NC_000913/2300729‑2300985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑