Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,414,977	T→C	G77G (GGT→GGC)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,414,977	0	T	C	100.0%	173.2 / NA	48	G77G (GGT→GGC)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/25);  total (23/25)

AGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGTAAGCACCAGTTTGCCCAGTCT  >  NC_000913/2414849‑2415109                                                                                                                                 |                                                                                                                                     aGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGCTGAAGGCCTGCTTTCCAg                                                                                                                           >  1:885484/1‑140 (MQ=255)  gCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGc                                                                                                                          <  2:870908/140‑1 (MQ=255)  gCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGc                                                                                                                          <  1:888981/140‑1 (MQ=255)  gCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGCTGAAGGCCTGCTTTCCAGc                                                                                                                          <  2:885484/140‑1 (MQ=255)        tCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAg                                                                                                                    >  2:951618/1‑140 (MQ=255)             ccTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGa                                                                                                               >  2:172342/1‑140 (MQ=255)             ccTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGa                                                                                                               <  2:370953/140‑1 (MQ=255)                aTCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGAtgt                                                                                                            <  2:710510/140‑1 (MQ=255)                  cGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGc                                                                                                          >  2:826554/1‑140 (MQ=255)                   gCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCCACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCt                                                                                                         <  2:93043/140‑1 (MQ=255)                     tCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGa                                                                                                       <  1:951618/140‑1 (MQ=255)                      cAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGAt                                                                                                      >  2:890575/1‑140 (MQ=255)                       aGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATg                                                                                                     >  2:113631/1‑140 (MQ=255)                        gCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGAtgt                                                                                                            >  1:805678/1‑132 (MQ=255)                        gCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGAtgt                                                                                                            <  2:805678/132‑1 (MQ=255)                           gcgTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAag                                                                                                 >  2:761799/1‑140 (MQ=255)                              tACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAgaga                                                                                              >  1:905335/1‑140 (MQ=255)                                 cGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGAtcg                                                                                           >  2:503960/1‑140 (MQ=255)                                   gTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGAtcgtc                                                                                         >  1:941131/1‑140 (MQ=255)                                      gCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCa                                                                                      <  1:761799/140‑1 (MQ=255)                                      gCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCa                                                                                      <  1:826554/140‑1 (MQ=255)                                       cGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCaa                                                                                     <  2:941131/140‑1 (MQ=255)                                       cGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCaa                                                                                     >  1:187782/1‑140 (MQ=255)                                            cgcCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTAc                                                                                <  2:670883/140‑1 (MQ=255)                                                         tACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCa                                                                   >  2:785154/1‑140 (MQ=255)                                                           cGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCaaa                                                                 <  2:905335/140‑1 (MQ=255)                                                           cGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCaaa                                                                 >  1:215001/1‑140 (MQ=255)                                                             aCTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGa                                                               <  1:113631/140‑1 (MQ=255)                                                             aCTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGa                                                               >  2:130383/1‑140 (MQ=255)                                                             aCTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGa                                                               >  1:315372/1‑140 (MQ=255)                                                                 tCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGc                                                                           >  2:431032/1‑124 (MQ=255)                                                                 tCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGc                                                                           <  1:431032/124‑1 (MQ=255)                                                                 tCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCt                                                           >  2:928301/1‑140 (MQ=255)                                                                 tCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCt                                                           >  2:375124/1‑140 (MQ=255)                                                                 tCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCt                                                           >  1:893765/1‑140 (MQ=255)                                                                          cgAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGtt                                                  <  1:543901/140‑1 (MQ=255)                                                                           gAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACAACGCTAACACCAAAGACGCTGAAGTCGTTc                                                 <  2:87553/140‑1 (MQ=255)                                                                                 ttCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTg                                           <  1:375124/140‑1 (MQ=255)                                                                                 ttCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTg                                           <  1:928301/140‑1 (MQ=255)                                                                                      ccacGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGt                                      >  1:647479/1‑140 (MQ=255)                                                                                         cgacgGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTg                                   >  2:423799/1‑140 (MQ=255)                                                                                                cgctgaaccgctgaaAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGa                            <  1:172342/140‑1 (MQ=255)                                                                                                 gctgaaccgctgaaAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGac                           <  2:645690/140‑1 (MQ=255)                                                                                                     aaccgctgaaAATGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGt                       <  2:187782/140‑1 (MQ=255)                                                                                                                aTGAGCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGTAAGCACCAGtt            <  2:647479/140‑1 (MQ=255)                                                                                                                    gCTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGTAAGCACCaccgccag        >  1:181463/1‑133 (MQ=255)                                                                                                                     cTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGTAAGCACCAGTTTGccc       <  2:315372/140‑1 (MQ=255)                                                                                                                     cTACGTTGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGTAAGCACCAGTTTGccc       <  1:785154/140‑1 (MQ=255)                                                                                                                          ttGAAGGCCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGTAAGCACCAGTTTGCCCAGtct  <  1:130383/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     AGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGTAAGCACCAGTTTGCCCAGTCT  >  NC_000913/2414849‑2415109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑