Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,500,102	G→A	V478I (GTC→ATC)	ypdA →	sensory histidine kinase YpdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,500,102	0	G	A	100.0%	301.0 / NA	85	V478I (GTC→ATC)	ypdA	sensory histidine kinase YpdA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/36);  total (49/36)

CTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAA  >  NC_000913/2499969‑2500235                                                                                                                                      |                                                                                                                                      cTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCa                                                                                                                                 >  2:593998/1‑140 (MQ=255)  tttGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCAt                                                                                                                                <  1:593998/140‑1 (MQ=255)      gTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGc                                                                                                                            >  1:238816/1‑140 (MQ=255)      gTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGc                                                                                                                            >  1:798748/1‑140 (MQ=255)         aCAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGtt                                                                                                                         <  2:238816/140‑1 (MQ=255)          cAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTg                                                                                                                        <  2:193009/140‑1 (MQ=255)           aaGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGc                                                                                                                       >  1:560098/1‑140 (MQ=255)           aaGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGc                                                                                                                       >  1:672690/1‑140 (MQ=255)            aGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCa                                                                                                                      <  2:798748/140‑1 (MQ=255)              cTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCaga                                                                                                                    >  1:590599/1‑140 (MQ=255)                     ttATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGa                                                                                                             >  2:786463/1‑140 (MQ=255)                        tCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCa                                                                                                                      <  2:571396/128‑1 (MQ=255)                        tCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCa                                                                                                                      >  1:571396/1‑128 (MQ=255)                               tatTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTAc                                                                                                   >  2:177976/1‑140 (MQ=255)                               tatTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTAc                                                                                                   >  1:333888/1‑140 (MQ=255)                               tatTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTAc                                                                                                   >  1:594009/1‑140 (MQ=255)                               tatTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTAc                                                                                                   >  1:492396/1‑140 (MQ=255)                                atTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACg                                                                                                  >  1:385911/1‑140 (MQ=255)                                 ttGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGc                                                                                                 <  2:662388/140‑1 (MQ=255)                                  tgatgaAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCa                                                                                                >  2:549733/1‑140 (MQ=255)                                   gatgaAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAt                                                                                               >  1:71263/1‑140 (MQ=255)                                    atgaAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt                                                                                              >  2:483424/1‑140 (MQ=255)                                    atgaAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt                                                                                              >  1:12511/1‑140 (MQ=255)                                    atgaAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCAtt                                                                                              >  1:326071/1‑140 (MQ=255)                                     tgaAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCGTCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTg                                                                                             <  2:276959/140‑1 (MQ=255)                                       aaGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg                                                                                           <  2:560098/140‑1 (MQ=255)                                       aaGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg                                                                                           <  2:672690/140‑1 (MQ=255)                                       aaGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg                                                                                           <  2:326071/140‑1 (MQ=255)                                       aaGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCg                                                                                           <  2:385911/140‑1 (MQ=255)                                        agagGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCgg                                                                                          >  1:886597/1‑140 (MQ=255)                                         gagGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTg                                                                                             >  1:352679/1‑136 (MQ=255)                                         gagGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTg                                                                                             <  2:352679/136‑1 (MQ=255)                                         gagGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGt                                                                                         <  2:21792/140‑1 (MQ=255)                                         gagGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGt                                                                                         >  1:576757/1‑140 (MQ=255)                                            gTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCg                                                                                      >  2:452357/1‑140 (MQ=255)                                            gTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCg                                                                                      <  2:333888/140‑1 (MQ=255)                                            gTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCg                                                                                      <  2:576757/140‑1 (MQ=255)                                            gTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCg                                                                                      <  1:483424/140‑1 (MQ=255)                                              gAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCgag                                                                                    >  1:707341/1‑140 (MQ=255)                                                    ctgcATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCg                                                                              >  1:186096/1‑140 (MQ=255)                                                       cATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGc                                                                           >  2:784713/1‑140 (MQ=255)                                                         ttCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCAc                                                                         >  1:833347/1‑140 (MQ=255)                                                              aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCa                                                                          <  2:375364/134‑1 (MQ=255)                                                              aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCa                                                                          >  1:375364/1‑134 (MQ=255)                                                              aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                                                    <  2:199241/140‑1 (MQ=255)                                                              aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                                                    <  2:833347/140‑1 (MQ=255)                                                              aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                                                    <  2:71263/140‑1 (MQ=255)                                                              aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                                                    <  2:734550/140‑1 (MQ=255)                                                              aGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                                                    <  1:452357/140‑1 (MQ=255)                                                                  tgctgATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGAt                                                                <  1:230649/140‑1 (MQ=255)                                                                    ctgATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATcc                                                              >  2:48744/1‑140 (MQ=255)                                                                      gATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCaa                                                            <  1:48744/140‑1 (MQ=255)                                                                      gATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCaa                                                            <  2:886597/140‑1 (MQ=255)                                                                      gATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCaa                                                            <  2:594009/140‑1 (MQ=255)                                                                       aTCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCaaa                                                           <  1:784713/140‑1 (MQ=255)                                                                       aTCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCaaa                                                           <  1:786463/140‑1 (MQ=255)                                                                           aGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                                                    >  1:84273/1‑127 (MQ=255)                                                                           aGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCAt                                                                    <  2:84273/127‑1 (MQ=255)                                                                                        gaACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCg                                          <  2:180742/140‑1 (MQ=255)                                                                                         aaCGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGa                                         >  2:119514/1‑140 (MQ=255)                                                                                                      aCGGTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATg                            >  1:210058/1‑140 (MQ=255)                                                                                                         gTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCg                         <  2:186096/140‑1 (MQ=255)                                                                                                         gTATTCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCg                         <  2:590599/140‑1 (MQ=255)                                                                                                            ttCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGAGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGc                      >  2:454142/1‑140 (MQ=255)                                                                                                             tCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCa                     >  1:874494/1‑140 (MQ=255)                                                                                                             tCAGCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCa                     >  2:510062/1‑140 (MQ=255)                                                                                                                gCCTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATa                  >  2:890282/1‑140 (MQ=255)                                                                                                                 ccTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATaa                 >  1:478509/1‑140 (MQ=255)                                                                                                                 ccTTGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATaa                 >  1:650597/1‑140 (MQ=255)                                                                                                                   ttGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATaaaa               <  1:549733/140‑1 (MQ=255)                                                                                                                    tGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAAt              >  2:425458/1‑140 (MQ=255)                                                                                                                    tGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAAt              >  1:360276/1‑140 (MQ=255)                                                                                                                    tGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAAt              >  1:604244/1‑140 (MQ=255)                                                                                                                    tGCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAAt              >  2:49940/1‑140 (MQ=255)                                                                                                                     gCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAAtt             <  2:478509/140‑1 (MQ=255)                                                                                                                     gCAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAAtt             <  2:650597/140‑1 (MQ=255)                                                                                                                      cAAAGGTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTg            >  2:276278/1‑140 (MQ=255)                                                                                                                           gTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCctg       <  1:890282/140‑1 (MQ=255)                                                                                                                           gTAAAGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCctg       >  2:267911/1‑140 (MQ=255)                                                                                                                             aaaGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCctgct     >  1:97930/1‑140 (MQ=255)                                                                                                                              aaGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCctgctg    >  1:692143/1‑140 (MQ=255)                                                                                                                               aGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGa   >  1:164247/1‑140 (MQ=255)                                                                                                                               aGGCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGa   >  1:125477/1‑140 (MQ=255)                                                                                                                                ggCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCTGGGCAATAAAATTGGCCTGCTGaa  >  2:305059/1‑140 (MQ=255)                                                                                                                                ggCGTTATCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGaa  >  2:63222/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      CTTTGGTGACAAGCTGACGGTTATCTATGATATTGATGAAGAGGTGAATTGCTGCATTCCCAGCCTGCTGATCCAGCCGTTGGTGGAGAACGCCATTGTCCACGGTATTCAGCCTTGCAAAGGTAAAGGCGTTGTCACCATCAGCGTTGCAGAGTGCGGAAATCGGGTACGCATTGCGGTGCGAGATACCGGGCACGGCATCGATCCAAAGGTGATTGAGCGGGTCGAAGCGAATGAAATGCCGGGCAATAAAATTGGCCTGCTGAA  >  NC_000913/2499969‑2500235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑