Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,511,625	G→A	A361V (GCT→GTT)	mntH ←	Mn(2(+))/Fe(2(+)): H(+) symporter MntH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,511,625	0	G	A	100.0%	238.9 / NA	68	A361V (GCT→GTT)	mntH	Mn(2(+))/Fe(2(+)): H(+) symporter MntH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/36);  total (32/36)

AACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGC  >  NC_000913/2511493‑2511760                                                                                                                                     |                                                                                                                                        aacaacCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATAcc                                                                                                                                  <  1:157747/140‑1 (MQ=255)  acaacCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCa                                                                                                                                 <  2:640782/140‑1 (MQ=255)     acCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAAGGATACCaaaa                                                                                                                              >  1:674434/1‑140 (MQ=255)     acCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCaaaa                                                                                                                              <  2:889240/140‑1 (MQ=255)     acCAGATATTAAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCaaaa                                                                                                                              <  1:675529/140‑1 (MQ=255)      ccAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAAc                                                                                                                             <  1:388680/140‑1 (MQ=255)        aGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCa                                                                                                                                 <  2:456805/134‑1 (MQ=255)        aGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCa                                                                                                                                 >  1:456805/1‑134 (MQ=255)          atatTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTaa                                                                                                                         >  2:209830/1‑140 (MQ=255)          atatTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTaa                                                                                                                         <  2:799818/140‑1 (MQ=255)             ttCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTAGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAg                                                                                                                      >  2:881702/1‑140 (MQ=255)              tCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACt                                                                                                                            <  1:742652/133‑1 (MQ=255)              tCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACt                                                                                                                            >  2:742652/1‑133 (MQ=255)              tCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGt                                                                                                                     >  2:766437/1‑140 (MQ=255)                aGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTAc                                                                                                                   >  2:687976/1‑140 (MQ=255)                aGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTAc                                                                                                                   >  1:668237/1‑140 (MQ=255)                  cgcCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCt                                                                                                                 <  1:626749/140‑1 (MQ=255)                    ccACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGa                                                                                                               <  1:682908/140‑1 (MQ=255)                     cACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGAc                                                                                                              >  1:18432/1‑140 (MQ=255)                      aCGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACt                                                                                                             <  1:881702/140‑1 (MQ=255)                       cGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTc                                                                                                            >  2:419479/1‑140 (MQ=255)                       cGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTc                                                                                                            >  2:489833/1‑140 (MQ=255)                        gACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCa                                                                                                           <  1:419479/140‑1 (MQ=255)                                  acaATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGTAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAAt                                                                                                 >  2:638439/1‑140 (MQ=255)                                    aaTCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATcc                                                                                               >  1:284176/1‑140 (MQ=255)                                        aCCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCgtgt                                                                                           <  2:955538/140‑1 (MQ=255)                                        aCCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCgtgt                                                                                           >  1:955538/1‑140 (MQ=255)                                        aCCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCgtgt                                                                                           >  1:345719/1‑140 (MQ=255)                                              ccTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATc                                                                                     >  2:43334/1‑140 (MQ=255)                                                       tttACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCAt                                                                            <  2:194663/140‑1 (MQ=255)                                                           cgcgTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCa                                                                          <  2:751344/138‑1 (MQ=255)                                                           cgcgTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCa                                                                          >  1:551958/1‑138 (MQ=255)                                                           cgcgTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCa                                                                          >  1:751344/1‑138 (MQ=255)                                                           cgcgTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCa                                                                          <  2:551958/138‑1 (MQ=255)                                                           cgcgTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGa                                                                        >  2:903092/1‑140 (MQ=255)                                                           cgcgTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGa                                                                        >  1:895884/1‑140 (MQ=255)                                                             cgTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGTAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAAt                                                                      <  1:638439/140‑1 (MQ=255)                                                               tttGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGa                                                                    <  2:345719/140‑1 (MQ=255)                                                                ttGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGAc                                                                   >  2:207637/1‑140 (MQ=255)                                                                ttGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGAc                                                                   >  2:433747/1‑140 (MQ=255)                                                                   cTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAAt                                                                <  2:18432/140‑1 (MQ=255)                                                                       tCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAAt                                                            <  2:821161/140‑1 (MQ=255)                                                                       tCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCAGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAAt                                                            <  1:209830/140‑1 (MQ=255)                                                                        cACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATg                                                           >  1:277719/1‑140 (MQ=255)                                                                         aCCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGa                                                          <  2:284176/140‑1 (MQ=255)                                                                         aCCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGa                                                          <  1:687976/140‑1 (MQ=255)                                                                           cAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACg                                                        <  1:903092/140‑1 (MQ=255)                                                                             gATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGc                                                      <  2:512653/140‑1 (MQ=255)                                                                                  cccATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACAt                                                 >  2:362691/1‑140 (MQ=255)                                                                                  cccATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACAt                                                 >  1:950241/1‑140 (MQ=255)                                                                                     aTCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGt                                              <  2:484320/140‑1 (MQ=255)                                                                                     aTCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGt                                              <  1:433747/140‑1 (MQ=255)                                                                                                gTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacgac                                   <  2:347980/140‑1 (MQ=255)                                                                                                 tCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacgacg                                  <  2:950241/140‑1 (MQ=255)                                                                                                       gTGAAAATCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCca                            >  1:789445/1‑140 (MQ=255)                                                                                                          aaaaTCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAg                         <  2:167951/140‑1 (MQ=255)                                                                                                          aaaaTCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAg                         <  1:766437/140‑1 (MQ=255)                                                                                                            aaTCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCg                       <  1:489833/140‑1 (MQ=255)                                                                                                            aaTCAGCAGTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCg                       <  1:589436/140‑1 (MQ=255)                                                                                                                 gcagTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGata                  >  1:829623/1‑140 (MQ=255)                                                                                                                 gcagTGGAACCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGata                  >  2:238124/1‑140 (MQ=255)                                                                                                                        aaCCAGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGc           <  2:668237/140‑1 (MQ=255)                                                                                                                            aGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAAt       >  1:635287/1‑140 (MQ=255)                                                                                                                            aGCGCCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAAt       >  1:265933/1‑140 (MQ=255)                                                                                                                              cgcCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATg      >  1:403328/1‑139 (MQ=255)                                                                                                                              cgcCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATg      <  2:403328/139‑1 (MQ=255)                                                                                                                               gcCAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGaa    >  1:865048/1‑140 (MQ=255)                                                                                                                                 cAGAACGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGc  <  1:362691/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        AACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGC  >  NC_000913/2511493‑2511760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑