Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,619,024	G→A	G379G (GGC→GGT)	uraA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,619,024	0	G	A	100.0%	173.4 / NA	50	G379G (GGC→GGT)	uraA	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/22);  total (28/22)

CGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGC  >  NC_000913/2618900‑2619154                                                                                                                             |                                                                                                                                   cGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCgatg                                                                                                                     <  2:447803/140‑1 (MQ=255) cGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCgatg                                                                                                                     >  1:667893/1‑140 (MQ=255)     cAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCa                                                                                                                 >  1:599486/1‑140 (MQ=255)      aGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCaa                                                                                                                <  2:769273/140‑1 (MQ=255)           cacTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCa                                                                                                           >  2:501532/1‑140 (MQ=255)             cttcttCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACg                                                                                                         >  1:923695/1‑140 (MQ=255)             cttcttCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACg                                                                                                         <  1:422660/140‑1 (MQ=255)              ttcttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACgg                                                                                                        <  1:581433/140‑1 (MQ=255)                cttcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGaa                                                                                                      >  2:260903/1‑140 (MQ=255)                  tcAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGt                                                                                                    >  2:379922/1‑140 (MQ=255)                     gggCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAg                                                                                                 >  2:616523/1‑140 (MQ=255)                        cgcAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGAt                                                                                              >  2:820944/1‑140 (MQ=255)                                     tCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCt                                                                                 <  2:667893/140‑1 (MQ=255)                                     tCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCt                                                                                 <  1:319116/140‑1 (MQ=255)                                      cAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCtt                                                                                <  1:210959/140‑1 (MQ=255)                                      cAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCtt                                                                                <  2:599486/140‑1 (MQ=255)                                         tttGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTttgt                                                                             >  1:90184/1‑140 (MQ=255)                                           tGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTttgttg                                                                           <  1:820944/140‑1 (MQ=255)                                              aGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTaa                                                                        >  1:510520/1‑140 (MQ=255)                                                   aaaCTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCtt                                                                                <  1:470611/127‑1 (MQ=255)                                                   aaaCTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCtt                                                                                >  2:470611/1‑127 (MQ=255)                                                   aaaCTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCAc                                                                   >  1:702857/1‑140 (MQ=255)                                                               aTACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTtcgattcgat                                                       >  1:585097/1‑140 (MQ=255)                                                                 aCCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATc                                                                      >  1:348039/1‑123 (MQ=255)                                                                 aCCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATc                                                                      <  2:348039/123‑1 (MQ=255)                                                                     acgaTGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAac                                                 >  1:926815/1‑140 (MQ=255)                                                                     acgaTGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAac                                                 >  2:713926/1‑140 (MQ=255)                                                                       gaTGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAacac                                               <  1:379922/140‑1 (MQ=255)                                                                          ggTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGaa                                            >  2:235171/1‑140 (MQ=255)                                                                                cAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGa                                             >  1:397818/1‑133 (MQ=255)                                                                                cAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGa                                             <  2:397818/133‑1 (MQ=255)                                                                                cAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCgg                                      <  2:923695/140‑1 (MQ=255)                                                                                 aGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGa                                     <  1:235171/140‑1 (MQ=255)                                                                                  gcgcCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGaa                                    <  2:510520/140‑1 (MQ=255)                                                                                        tACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGaa                                    >  2:543122/1‑134 (MQ=255)                                                                                        tACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGaa                                    <  1:543122/134‑1 (MQ=255)                                                                                          ccTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAg                                   <  2:431434/133‑1 (MQ=255)                                                                                          ccTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAg                                   >  1:431434/1‑133 (MQ=255)                                                                                          ccTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGAt                            <  2:90184/140‑1 (MQ=255)                                                                                          ccTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGAt                            <  2:713011/140‑1 (MQ=255)                                                                                                aaCTCCGCCGCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACAcc                      >  2:293319/1‑140 (MQ=255)                                                                                                    ccgccgCGCCGATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATaa                  >  2:226213/1‑140 (MQ=255)                                                                                                     cgccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATaaa                 >  1:4914/1‑140 (MQ=255)                                                                                                     cgccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATaaa                 >  2:741641/1‑140 (MQ=255)                                                                                                     cgccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATaaa                 >  1:433941/1‑140 (MQ=255)                                                                                                     cgccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATaaa                 >  2:317331/1‑140 (MQ=255)                                                                                                       ccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAagc               >  1:749617/1‑140 (MQ=255)                                                                                                       ccgcgccgATGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAagc               >  1:19625/1‑140 (MQ=255)                                                                                                               aTGTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGa       <  1:681460/140‑1 (MQ=255)                                                                                                                 gTTTACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGaaa     <  2:702857/140‑1 (MQ=255)                                                                                                                    tACCTTCGCACCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAAcgc  <  2:690533/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                   CGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGC  >  NC_000913/2618900‑2619154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑