Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,694,252	T→C	N431S (AAC→AGC)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,694,252	0	T	C	100.0%	60.8 / NA	18	N431S (AAC→AGC)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/4);  total (14/4)

GTTGTCGCGCTGTACGGAAGCAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTT  >  NC_000913/2694122‑2694388                                                                                                                                   |                                                                                                                                         gTTGTCGCGCTGTACGGAAGCAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGc                                                                                                                                 <  1:732628/140‑1 (MQ=255)                    gCAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGgc                                                                                                              <  2:663001/140‑1 (MQ=255)                     cAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGgcg                                                                                                             <  1:235996/140‑1 (MQ=255)                     cAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGgcg                                                                                                             >  2:588863/1‑140 (MQ=255)                                                   aCGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATg                                                                               >  1:321409/1‑140 (MQ=255)                                                   aCGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATg                                                                               >  2:956776/1‑140 (MQ=255)                                                        aTAGAAGCCCCTGCCCCCCCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGTCGATCCCGCCCGCCAGCATGATCgg                                                                          <  2:372348/140‑1 (MQ=255)                                                                 gCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGTCGATCCCGCCCGCCAGCATGATCGGTTTGTGATa                                                                 >  1:372348/1‑140 (MQ=255)                                                                 gCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATa                                                                 >  2:372403/1‑140 (MQ=255)                                                                     caccacCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACcg                                                             >  1:585382/1‑140 (MQ=255)                                                                             ccAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGctc                                                     >  1:283642/1‑140 (MQ=255)                                                                               aaGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCtt                                                   >  2:868387/1‑140 (MQ=255)                                                                                              tGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGt                                    >  1:903263/1‑140 (MQ=255)                                                                                              tGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGt                                    >  1:508837/1‑140 (MQ=255)                                                                                                   ggCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACt                               >  1:565239/1‑140 (MQ=255)                                                                                                   ggCCGCCGAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACt                               >  2:932575/1‑140 (MQ=255)                                                                                                        ccgAGACCGCCCAGCTCCCCCCCGACGCTGTCCTCCCTTTTTTGTACGTGATCGGCGCAAATGTTGCCGCCCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTttc                          <  2:758137/140‑1 (MQ=255)                                                                                                        ccgAGAACGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTttc                          >  1:758137/1‑140 (MQ=255)                                                                                                             aaCGCCCAGCTTCGCCCCGACGCTGTTCTCCCTTTTTTGTCCGTGATCGGCGCGATTGTTGCCGCCCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCAt                     <  2:565239/140‑1 (MQ=255)                                                                                                             aaCGACCAGCTTCGCCCCGACGCTGATCTCGCTTTTTTGTACGTGATCGGCGCGATTGTTCCCGACCCCCCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCAt                     <  2:766394/140‑1 (MQ=255)                                                                                                             aaCGACCAGCTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCAt                     <  1:372403/140‑1 (MQ=255)                                                                                                             aaCGACCACCTCCCCCCCGACGCTGATCTCGCCTTTTTGTACGTGAGCGGCGCGAATGTTGCCGCCCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCAt                     <  2:283642/140‑1 (MQ=255)                                                                                                                  ccAGCTTCGCACCGACGCTGATCTCGCTTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTa                <  2:178314/140‑1 (MQ=255)                                                                                                                      cTTCGCACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGa            >  1:766394/1‑140 (MQ=255)                                                                                                                           ccccGACGTTGTTCTCGCTTTTTTGTCCGTGATGGGCGCGATTGTCGCCGACCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAg       <  2:903263/138‑1 (MQ=255)                                                                                                                           cACCGACGCTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAg       <  1:868387/140‑1 (MQ=255)                                                                                                                                gcGTTGTTCTCCTTTTTTTTTGCGTGTCGGGGGCGATTGTGGCGGACCCCACCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGtt  <  2:144156/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         GTTGTCGCGCTGTACGGAAGCAAAGTCGAGGTCGGCATCAGACTGACCAGACGCCATAGAAGACGCTGCACCACCGCCAAGACCGATGTTCATTGCCGGGCCGCCGAGAACGACCAGCTTCGCACCGACGTTGATCTCGCCTTTTTGTACGTGATCGGCGCGAATGTTGCCGATCCCGCCCGCCAGCATGATCGGTTTGTGATAACCGCGCAGCTCTTCGCCGTTGTGGCTGTTCACTTTTTCTTCATAAGTACGGAAGTAGCCGTT  >  NC_000913/2694122‑2694388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑