Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,711,316	T→C	H299H (CAT→CAC)	nadB →	L‑aspartate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,711,316	0	T	C	100.0%	147.5 / NA	42	H299H (CAT→CAC)	nadB	L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/18);  total (24/18)

GCAATTTCCTGTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCATGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGAACCGGTA  >  NC_000913/2711180‑2711451                                                                                                                                         |                                                                                                                                        gCAATTTCCTGTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCacgaa                                                                                                                                      >  1:19311/1‑140 (MQ=255) gCAATTTCCTGTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCacgaa                                                                                                                                      >  2:705165/1‑140 (MQ=255)       tCCTGTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCAcgaaatgaaa                                                                                                                                <  1:705165/140‑1 (MQ=255)           gTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGcc                                                                                                                            <  2:245015/140‑1 (MQ=255)               aCAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCgg                                                                                                                        >  2:900083/1‑140 (MQ=255)                 aGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGgcg                                                                                                                      <  2:577271/140‑1 (MQ=255)                  gAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGgcgc                                                                                                                     <  2:592163/140‑1 (MQ=255)                   aaGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGgcg                                                                                                                      >  1:337320/1‑138 (MQ=255)                   aaGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGgcg                                                                                                                      <  2:337320/138‑1 (MQ=255)                      cACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGAt                                                                                                                 >  2:738131/1‑140 (MQ=255)                           cgcgGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTAt                                                                                                            >  1:43715/1‑140 (MQ=255)                            gcggcgAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATg                                                                                                           <  1:812683/140‑1 (MQ=255)                            gcggcgAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATg                                                                                                           <  2:43715/140‑1 (MQ=255)                                 gAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCt                                                                                                      <  1:955620/140‑1 (MQ=255)                                   aGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTg                                                                                                    >  1:94786/1‑140 (MQ=255)                                        cTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATc                                                                                               <  2:94786/140‑1 (MQ=255)                                                     cccGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCccg                                                                                  >  2:86779/1‑140 (MQ=255)                                                              tACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCTGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTa                                                                         >  1:314124/1‑140 (MQ=255)                                                               aCGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTAt                                                                        >  1:762852/1‑140 (MQ=255)                                                                cgcgTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTAtt                                                                       >  1:150778/1‑140 (MQ=255)                                                                      ttATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAg                                                                 >  2:546693/1‑140 (MQ=255)                                                                               aTTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCg                                                        >  1:376010/1‑140 (MQ=255)                                                                                 tttGATGAGCGCGGCGAACTGGCCCTGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCgat                                                      <  2:314124/140‑1 (MQ=255)                                                                                          cgcgGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGa                                             >  1:849542/1‑140 (MQ=255)                                                                                          cgcgGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGa                                             >  1:474394/1‑140 (MQ=255)                                                                                          cgcgGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGa                                             >  1:573764/1‑140 (MQ=255)                                                                                          cgcgGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGa                                             >  1:725220/1‑140 (MQ=255)                                                                                            cggcgAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCgatgat                                                   <  2:715849/132‑1 (MQ=255)                                                                                            cggcgAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCgatgat                                                   >  1:715849/1‑132 (MQ=255)                                                                                                   cTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAgctgct                                    <  2:474394/140‑1 (MQ=255)                                                                                                        cccGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGc                               <  2:376010/140‑1 (MQ=255)                                                                                                         ccGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCt                              <  2:725220/140‑1 (MQ=255)                                                                                                         ccGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCt                              >  2:658012/1‑140 (MQ=255)                                                                                                           gcgcgATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTgg                            >  2:960319/1‑140 (MQ=255)                                                                                                              cgATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGa                         >  1:878934/1‑140 (MQ=255)                                                                                                               gATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGAt                        <  1:279889/140‑1 (MQ=255)                                                                                                                atatTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGAtt                       >  2:658350/1‑140 (MQ=255)                                                                                                                      tCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAtctc                 >  1:2135/1‑140 (MQ=255)                                                                                                                      tCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAtctc                 >  1:149033/1‑140 (MQ=255)                                                                                                                           cgcgCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTcacaca            <  1:900083/140‑1 (MQ=255)                                                                                                                             cgcCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAg          >  1:83433/1‑140 (MQ=255)                                                                                                                               ccATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGaa        <  2:2135/140‑1 (MQ=255)                                                                                                                               ccATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGaa        <  2:149033/140‑1 (MQ=255)                                                                                                                               ccATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGaa        <  2:762852/140‑1 (MQ=255)                                                                                                                                     aCCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGAACCGGTa  <  1:738131/140‑1 (MQ=255)                                                                                                                                     aCCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGAACCGGTa  >  1:866574/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GCAATTTCCTGTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCATGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGAACCGGTA  >  NC_000913/2711180‑2711451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑