Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,885	G→A	intergenic (‑25/+174)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,885	0	G	A	100.0%	178.9 / NA	50	intergenic (‑25/+174)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/31);  total (19/31)

CCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTA  >  NC_000913/2817756‑2818019                                                                                                                                  |                                                                                                                                        ccTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCACTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATAc                                                                                                                               <  1:905422/140‑1 (MQ=255)  cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATAcc                                                                                                                              >  2:555670/1‑140 (MQ=255)  cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATAcc                                                                                                                              >  1:220499/1‑140 (MQ=255)  cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATAcc                                                                                                                              <  1:366455/140‑1 (MQ=255)  cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATAcc                                                                                                                              >  1:859474/1‑140 (MQ=255)   ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCg                                                                                                                             <  1:880984/140‑1 (MQ=255)   ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCg                                                                                                                             <  2:785126/140‑1 (MQ=255)    tCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGc                                                                                                                            >  2:405934/1‑140 (MQ=255)        tGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGctact                                                                                                                        <  2:910145/140‑1 (MQ=255)        tGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGctact                                                                                                                        <  1:895960/140‑1 (MQ=255)        tGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGctact                                                                                                                        <  1:773863/140‑1 (MQ=255)                 ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:124647/140‑1 (MQ=255)                 ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:866550/140‑1 (MQ=255)                 ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:555670/140‑1 (MQ=255)                 ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:454836/140‑1 (MQ=255)                 ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:405934/140‑1 (MQ=255)                  tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              <  2:273826/140‑1 (MQ=255)                  tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              <  1:227941/140‑1 (MQ=255)                  tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              <  2:810908/140‑1 (MQ=255)                           tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAaga                                                                                                     >  1:810909/1‑140 (MQ=255)                                tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              >  2:277880/1‑126 (MQ=255)                                tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              >  2:821880/1‑126 (MQ=255)                                tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              <  1:277880/126‑1 (MQ=255)                                tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              <  1:821880/126‑1 (MQ=255)                                tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATT‑ATACCGCTACTAAAGCCATATCAATTAAGAGATGGt                                                                                               >  1:597948/1‑140 (MQ=255)                                   aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               >  2:765213/1‑122 (MQ=255)                                   aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:765213/122‑1 (MQ=255)                                   aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGc                                                                                             >  1:635774/1‑140 (MQ=255)                                     ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTTCTCATTCCAGTTGAGCTACGGCTGCATCGGGAAACCTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATTAATTAAGAGATGGCGCAt                                                                                           >  2:363364/1‑140 (MQ=14)                                     ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCAt                                                                                           >  2:782964/1‑140 (MQ=255)                                     ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATT‑ATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATc                                                                                          <  2:597948/140‑1 (MQ=255)                                            gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGggag                                                                                    <  1:888346/140‑1 (MQ=255)                                                       ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGcc                                                                         <  2:810909/140‑1 (MQ=255)                                                               tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCt                                                                 >  1:657488/1‑140 (MQ=255)                                                                 ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                               <  2:635774/140‑1 (MQ=255)                                                                 ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                               <  1:566180/140‑1 (MQ=255)                                                                                  tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCt                                             <  2:657488/140‑1 (MQ=255)                                                                                    tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGTCCGTTgtaa                                            >  2:225656/1‑137 (MQ=255)                                                                                       ccAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑cc                                                   <  2:98939/129‑1 (MQ=255)                                                                                       ccAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑cc                                                   >  1:98939/1‑129 (MQ=255)                                                                                        cAGCTGAGCTACGGATGCATCGGGAAAACTTATTCT‑CTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCaa                                      <  1:165295/140‑1 (MQ=255)                                                                                           cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACg                                    >  1:614242/1‑140 (MQ=255)                                                                                             gAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGtt                                  <  2:212054/140‑1 (MQ=255)                                                                                                cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATGAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTatc                               >  2:878413/1‑140 (MQ=255)                                                                                                cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACg                                    >  2:209283/1‑135 (MQ=255)                                                                                                cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACg                                    <  1:209283/135‑1 (MQ=255)                                                                                                cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTat                                <  2:325862/139‑1 (MQ=255)                                                                                                cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTat                                >  1:325862/1‑139 (MQ=255)                                                                                                cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAACAACGTTatc                               >  1:180430/1‑140 (MQ=255)                                                                                                             gggAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                  <  1:878413/140‑1 (MQ=255)                                                                                                                            aCTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt   <  2:712733/140‑1 (MQ=255)                                                                                                                             ctgctaATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt   >  2:135144/1‑139 (MQ=255)                                                                                                                             ctgctaATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt   <  1:135144/139‑1 (MQ=255)                                                                                                                             ctgctaATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTa  >  2:243386/1‑140 (MQ=255)                                                                                                                             ctgctaATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTa  >  1:791011/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        CCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTA  >  NC_000913/2817756‑2818019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑