Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,865,733	A→G	V211V (GTA→GTG)	ygbN →	putative transporter YgbN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,865,733	0	A	G	100.0%	165.8 / NA	46	V211V (GTA→GTG)	ygbN	putative transporter YgbN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/24);  total (22/24)

GGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTACTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCG  >  NC_000913/2865604‑2865859                                                                                                                                  |                                                                                                                               ggggTTACTCCACGCAGACATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGa                                                                                                                      <  1:2960/140‑1 (MQ=255)   ggTTACTCCACGCAGACATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATg                                                                                                                    <  1:120507/140‑1 (MQ=255)       aCTCCACGCAGACATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAAc                                                                                                                <  2:162341/140‑1 (MQ=255)       aCTCCACGCAGACATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAAc                                                                                                                <  1:584450/140‑1 (MQ=255)             cGCAGACATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTc                                                                                                          >  2:978301/1‑140 (MQ=255)                aGACATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCgg                                                                                                       <  1:367368/140‑1 (MQ=255)                  aCATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGcc                                                                                                     <  1:211128/140‑1 (MQ=255)                   cATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCgg                                                                                                       <  2:655546/137‑1 (MQ=255)                   cATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCgg                                                                                                       >  1:655546/1‑137 (MQ=255)                            gctAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCgg                                                                                                       >  1:960839/1‑128 (MQ=255)                            gctAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCgg                                                                                                       <  2:960839/128‑1 (MQ=255)                            gctAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAg                                                                                           >  1:663262/1‑140 (MQ=255)                            gctAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAg                                                                                           >  1:832353/1‑140 (MQ=255)                              tAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGgc                                                                                         <  1:957896/140‑1 (MQ=255)                                      atcGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACaaa                                                                                 >  1:54735/1‑140 (MQ=255)                                      atcGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACaaa                                                                                 >  1:448487/1‑140 (MQ=255)                                          gTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTa                                                                             >  2:251096/1‑140 (MQ=255)                                          gTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTa                                                                             <  2:448487/140‑1 (MQ=255)                                            aTTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAg                                                                           >  2:168393/1‑140 (MQ=255)                                                cGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGat                                                                       <  1:677782/140‑1 (MQ=255)                                                         gacaggTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAaaataaat                                                              <  2:650951/135‑1 (MQ=255)                                                                    gttgttGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCg                                                    <  2:411289/139‑1 (MQ=255)                                                                    gttgttGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCg                                                    >  1:411289/1‑139 (MQ=255)                                                                       gttgGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTcgc                                                >  1:527030/1‑140 (MQ=255)                                                                       gttgGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTcgc                                                <  2:54735/140‑1 (MQ=255)                                                                        ttgGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTcgcg                                               <  2:746787/140‑1 (MQ=255)                                                                               cTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCa                                        <  1:727544/140‑1 (MQ=255)                                                                                     aGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGc                                  >  1:835134/1‑140 (MQ=255)                                                                                     aGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGc                                  >  1:626647/1‑140 (MQ=255)                                                                                     aGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGc                                  >  1:219097/1‑140 (MQ=255)                                                                                     aGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGc                                  >  2:466089/1‑140 (MQ=255)                                                                                      gCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCt                                 <  2:663262/140‑1 (MQ=255)                                                                                      gCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCt                                 <  2:219097/140‑1 (MQ=255)                                                                                       cGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTa                                <  2:527030/140‑1 (MQ=255)                                                                                        gAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTaa                               <  1:168393/140‑1 (MQ=255)                                                                                                tCAATAAGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTAtt                       >  1:866361/1‑140 (MQ=255)                                                                                                     aaGCGCCAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTAt                  <  1:466089/140‑1 (MQ=255)                                                                                                          ccAATATGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGa             >  2:953463/1‑140 (MQ=255)                                                                                                              tatGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTAt         >  1:945319/1‑140 (MQ=255)                                                                                                              tatGCGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTAt         >  1:4833/1‑140 (MQ=255)                                                                                                                  cGATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATg     <  1:953463/140‑1 (MQ=255)                                                                                                                   gATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATgg    >  2:565737/1‑140 (MQ=255)                                                                                                                   gATGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATgg    >  1:745388/1‑140 (MQ=255)                                                                                                                     tGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCTTATcgc               <  1:812290/127‑1 (MQ=255)                                                                                                                     tGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCTTATcgc               >  2:812290/1‑127 (MQ=255)                                                                                                                     tGTCAGTAGAAGTGCTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCg  <  2:745388/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                               GGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCGGTATTGCGATTTCTATTCCCGTAGGGGTTGTTGGCTACTTTGCAGCGAAAATAATCAATAAGCGCCAATATGCGATGTCAGTAGAAGTACTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCG  >  NC_000913/2865604‑2865859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑