Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,908,002	T→C	intergenic (‑61/+27)	eno ← / ← pyrG	enolase/CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,908,002	0	T	C	100.0%	116.0 / NA	33	intergenic (‑61/+27)	eno/pyrG	enolase/CTP synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/19);  total (14/19)

TACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAA  >  NC_000913/2907867‑2908127                                                                                                                                        |                                                                                                                              tACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCAcgc                                                                                                                           >  1:379758/1‑140 (MQ=255)   cTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGt                                                                                                                         >  2:180690/1‑140 (MQ=255)     tCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTg                                                                                                                       <  2:724512/140‑1 (MQ=255)         cTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGc                                                                                                                   >  2:38828/1‑140 (MQ=255)         cTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGc                                                                                                                   >  1:579115/1‑140 (MQ=255)                aGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACt                                                                                                            <  2:95253/140‑1 (MQ=255)                  tCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACttt                                                                                                          <  2:379758/140‑1 (MQ=255)                  tCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACttt                                                                                                          <  1:603751/140‑1 (MQ=255)                      gTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTtta                                                                                                      >  1:28398/1‑140 (MQ=255)                                   tCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTtacttact                                                                                                >  2:293020/1‑133 (MQ=255)                                   tCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTtacttact                                                                                                <  1:293020/133‑1 (MQ=255)                                     gatgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGa                                                                                        <  1:67713/139‑1 (MQ=255)                                     gatgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGa                                                                                        >  2:67713/1‑139 (MQ=255)                                             cACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTgg                                                                               >  2:796352/1‑140 (MQ=255)                                                      tgatTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTg                                                                      <  1:796352/140‑1 (MQ=255)                                                        atTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggc                                                                    <  2:343928/140‑1 (MQ=255)                                                            ttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCt                                                                <  1:605680/140‑1 (MQ=255)                                                                 aTTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAAC‑‑TTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACaa                                                         <  2:419796/140‑1 (MQ=255)                                                                   tttGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACaa                                                         <  1:643905/140‑1 (MQ=255)                                                                   tttGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACaa                                                         <  1:38828/140‑1 (MQ=255)                                                                                    ccTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCggg                                        >  2:983270/1‑140 (MQ=255)                                                                                             aCTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCa                               >  1:688880/1‑140 (MQ=255)                                                                                              cTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCAc                              <  2:695819/140‑1 (MQ=255)                                                                                               tAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg                             <  2:579115/140‑1 (MQ=255)                                                                                                 gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTg                           <  1:180690/140‑1 (MQ=255)                                                                                                                cAGACAAACAACGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACt            >  1:47338/1‑140 (MQ=255)                                                                                                                     aaacaaCGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa       <  2:28398/140‑1 (MQ=255)                                                                                                                        caacGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATgg    >  2:417263/1‑140 (MQ=255)                                                                                                                          acGCGTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGaa  <  2:47338/140‑1 (MQ=255)                                                                                                                           cgcgTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACt            <  2:294409/129‑1 (MQ=255)                                                                                                                           cgcgTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACt            >  1:294409/1‑129 (MQ=255)                                                                                                                             cgTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGaa  <  2:453513/137‑1 (MQ=255)                                                                                                                             cgTACCCAGGGCACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGaa  >  1:453513/1‑137 (MQ=255)                                                                                                                                        |                                                                                                                              TACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAA  >  NC_000913/2907867‑2908127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑