Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,993,230	A→G	A379A (GCA→GCG)	ygeH →	putative transcriptional regulator YgeH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,993,230	0	A	G	100.0%	213.0 / NA	58	A379A (GCA→GCG)	ygeH	putative transcriptional regulator YgeH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/26);  total (32/26)

CTGTCGATGGAAAAATTTTAGCTATTATGGGACTGATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCA  >  NC_000913/2993097‑2993363                                                                                                                                      |                                                                                                                                      cTGTCGATGGAAAAATTTTAGCTATTATGGGACTGATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTc                                                                                                                                 >  2:551849/1‑140 (MQ=255)  tGTCGATGGAAAAATTTTAGCTATTATGGGACTGATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTcc                                                                                                                                >  2:893384/1‑140 (MQ=255)  tGTCGATGGAAAAATTTTAGCTATTATGGGACTGATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTcc                                                                                                                                >  2:173826/1‑140 (MQ=255)       aTGGAAAAATTTTAGCTATTATGGGACTGATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACttt                                                                                                                           >  2:127343/1‑140 (MQ=255)         ggAAAAATTTTAGCTATTATGGGACTGATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCa                                                                                                                         >  1:132005/1‑140 (MQ=255)                               gACTGATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCa                                                                                                   <  2:588016/140‑1 (MQ=255)                                  tGATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAgg                                                                                                <  1:173826/140‑1 (MQ=255)                                  tGATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAgg                                                                                                <  1:893384/140‑1 (MQ=255)                                   gATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGa                                                                                               <  2:931269/140‑1 (MQ=255)                                   gATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGa                                                                                               >  1:943828/1‑140 (MQ=255)                                   gATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGa                                                                                               <  1:349996/140‑1 (MQ=255)                                    aTAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGAt                                                                                              <  2:95585/140‑1 (MQ=255)                                    aTAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGAt                                                                                              >  1:95585/1‑140 (MQ=255)                                              tgtctgGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGAc                                                                                    >  1:742854/1‑140 (MQ=255)                                                                     cATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCag                                                             <  1:127343/140‑1 (MQ=255)                                                                      atatCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCaga                                                            <  2:188338/140‑1 (MQ=255)                                                                          cTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGAc                                                        >  2:588664/1‑140 (MQ=255)                                                                              tttGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGaaa                                                    >  2:90153/1‑140 (MQ=255)                                                                                  aaCAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCa                                                >  1:18858/1‑140 (MQ=255)                                                                                  aaCAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCa                                                >  1:216422/1‑140 (MQ=255)                                                                                       gCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTtgt                                           >  2:940971/1‑140 (MQ=255)                                                                                       gCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTtgt                                           >  1:976452/1‑140 (MQ=255)                                                                                             aTACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATaaa                                     <  1:90153/140‑1 (MQ=255)                                                                                                cacTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGa                                  <  1:205347/140‑1 (MQ=255)                                                                                                 acTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGaa                                 >  2:865542/1‑140 (MQ=255)                                                                                                 acTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGaa                                 >  2:292584/1‑140 (MQ=255)                                                                                                 acTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGaa                                 >  1:71093/1‑140 (MQ=255)                                                                                                 acTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGa                                  >  1:954802/1‑139 (MQ=255)                                                                                                 acTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGa                                  <  2:954802/139‑1 (MQ=255)                                                                                                 acTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAAACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGaa                                 >  2:479340/1‑140 (MQ=255)                                                                                                 acTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGACAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGaa                                 >  2:684710/1‑140 (MQ=255)                                                                                                  cTCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAAt                                <  1:940971/140‑1 (MQ=255)                                                                                                   tCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAAt                                >  2:647990/1‑139 (MQ=255)                                                                                                   tCAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAAt                                <  1:647990/139‑1 (MQ=255)                                                                                                    cAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAAtgt                              <  1:200434/140‑1 (MQ=255)                                                                                                    cAACTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAAACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAAtgt                              <  1:479340/140‑1 (MQ=255)                                                                                                     aaCTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAAtgtg                             <  1:588664/140‑1 (MQ=255)                                                                                                       cTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGa                                  <  1:757716/133‑1 (MQ=255)                                                                                                       cTGATATAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGa                                  >  2:757716/1‑133 (MQ=255)                                                                                                          atatAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATaaa                                     >  2:197922/1‑127 (MQ=255)                                                                                                          atatAGCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATaaa                                     <  1:197922/127‑1 (MQ=255)                                                                                                               gCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGTAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgta                   <  2:132005/140‑1 (MQ=255)                                                                                                               gCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgta                   <  2:18858/140‑1 (MQ=255)                                                                                                               gCCTCTCTCTACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgta                   <  2:742854/140‑1 (MQ=255)                                                                                                                    tctctACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgta                   <  1:853865/135‑1 (MQ=255)                                                                                                                    tctctACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgta                   >  2:853865/1‑135 (MQ=255)                                                                                                                    tctctACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGc              >  1:670902/1‑140 (MQ=255)                                                                                                                     ctctACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgta                   >  1:349868/1‑134 (MQ=255)                                                                                                                     ctctACTACTATAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgta                   <  2:349868/134‑1 (MQ=255)                                                                                                                          ctactaTAGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAAcc        <  1:292584/140‑1 (MQ=255)                                                                                                                                taGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgta                   <  1:189204/123‑1 (MQ=255)                                                                                                                                taGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgta                   >  2:189204/1‑123 (MQ=255)                                                                                                                                taGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCa  >  2:118338/1‑140 (MQ=255)                                                                                                                                taGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCa  >  1:428208/1‑140 (MQ=255)                                                                                                                                taGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCa  >  2:92842/1‑140 (MQ=255)                                                                                                                                taGGGCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCa  >  2:494298/1‑140 (MQ=255)                                                                                                                                  gggCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgtat                  >  2:709300/1‑122 (MQ=255)                                                                                                                                  gggCGCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgtat                  <  1:709300/122‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CTGTCGATGGAAAAATTTTAGCTATTATGGGACTGATAACTGGTCTGTCTGGACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCA  >  NC_000913/2993097‑2993363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑