Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,556,804	(T)6→5	intergenic (+7/‑109)	ytiD → / → idlP	protein YtiD/iraD leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,556,799	0	T	.	100.0%	285.5 / NA	67	intergenic (+2/‑114)	ytiD/idlP	protein YtiD/iraD leader peptide
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (28/39);  total (28/39)

GATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCAT  >  NC_000913/4556660‑4556927                                                                                                                                            |                                                                                                                                 gATTATGCGGAAATATATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAAAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACt                                                                                                                                  >  1:291269/1‑140 (MQ=255)  aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtt                                                                                                                                 >  2:170866/1‑140 (MQ=255)  aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtt                                                                                                                                 >  2:718675/1‑140 (MQ=255)  aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtt                                                                                                                                 >  2:112222/1‑140 (MQ=255)        cGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTATCTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtttttcct                                                                                                                           <  1:170866/140‑4 (MQ=255)        cGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtttttcct                                                                                                                           <  1:697013/140‑4 (MQ=255)          gAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGa                                                                                                                        <  1:614469/140‑1 (MQ=255)               aaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGt                                                                                                                   <  2:148857/140‑1 (MQ=255)                 ataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCa                                                                                                                 <  1:708292/140‑1 (MQ=255)                  taatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAg                                                                                                                <  1:115314/140‑1 (MQ=255)                  taatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAg                                                                                                                <  2:715536/140‑1 (MQ=255)                     tttCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAg                                                                                                             <  2:239978/140‑1 (MQ=255)                      ttCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGa                                                                                                            <  2:118790/140‑1 (MQ=255)                            ccGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCa                                                                                                      <  1:812298/140‑1 (MQ=255)                            ccGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAATTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCa                                                                                                      >  1:106538/1‑140 (MQ=255)                                  ttAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCa                                                                                                <  2:52671/140‑1 (MQ=255)                                      gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                                                            <  1:270016/140‑1 (MQ=255)                                      gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                                                            <  1:150339/140‑1 (MQ=255)                                        agcagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAg                                                                                          <  2:473658/140‑1 (MQ=255)                                          cagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGt                                                                                        >  1:106655/1‑140 (MQ=255)                                          cagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGt                                                                                        >  1:423326/1‑140 (MQ=255)                                                tGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtata                                                                                  >  1:542143/1‑140 (MQ=255)                                                tGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtata                                                                                  >  1:797811/1‑140 (MQ=255)                                                         ttttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGa                                                                         <  2:5464/140‑1 (MQ=255)                                                          tttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGAc                                                                        >  1:645145/1‑140 (MQ=255)                                                           ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                                                       <  2:447854/140‑1 (MQ=255)                                                           ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                                                       <  2:645145/140‑1 (MQ=255)                                                           ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                                                       <  1:44132/140‑1 (MQ=255)                                                           ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGCAAGTAGATATTATAAGGAAGGCAGGGTTTTAAACTTTCCtaaaa                                                                       >  1:604206/1‑136 (MQ=255)                                                           ttCATCTACGTAACTATTCGGAATATTCAAAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                                                       <  2:291269/140‑1 (MQ=255)                                                                 tACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTc                                                                 >  1:58520/1‑140 (MQ=255)                                                                 tACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTCTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTc                                                                 >  2:253009/1‑140 (MQ=255)                                                                   cGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGAt                                                                   >  1:510267/1‑136 (MQ=255)                                                                   cGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGAt                                                                   <  2:510267/136‑1 (MQ=255)                                                                          aTTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtc                                                        <  2:312992/140‑1 (MQ=255)                                                                          aTTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtc                                                        >  2:718396/1‑140 (MQ=255)                                                                           ttCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtct                                                       <  1:331192/140‑1 (MQ=255)                                                                                aaTATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGAc                                                  <  1:11609/140‑1 (MQ=255)                                                                                     tCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa                                             >  2:172640/1‑140 (MQ=255)                                                                                     tCAGAATATACTAGCGGTTTTTTTCTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCGGGCCTGTCTCAGACTGACa                                             <  1:253009/140‑1 (MQ=255)                                                                                         aaTATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGa                                         >  1:33681/1‑140 (MQ=255)                                                                                          atatACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGAt                                        >  2:522662/1‑140 (MQ=255)                                                                                             taCTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTa                                     <  1:112222/140‑1 (MQ=255)                                                                                              aCTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa                                             <  1:697261/131‑1 (MQ=255)                                                                                              aCTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa                                             >  2:697261/1‑131 (MQ=255)                                                                                              aCTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa                                    >  2:254967/1‑140 (MQ=255)                                                                                              aCTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa                                    >  1:327767/1‑140 (MQ=255)                                                                                              aCTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa                                    >  1:619225/1‑140 (MQ=255)                                                                                                 gggggTTTTTTTTGGATTTTGAT‑AATTTTAACAAAACCTGGC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGaa                                <  2:604206/137‑1 (MQ=255)                                                                                                   cGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGaa                                >  1:174290/1‑139 (MQ=255)                                                                                                   cGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGaa                                <  2:174290/139‑1 (MQ=255)                                                                                                   cGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAAc                               >  1:156080/1‑140 (MQ=255)                                                                                                   cGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAAc                               >  1:1957/1‑140 (MQ=255)                                                                                                     gTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACaa                             >  2:118057/1‑140 (MQ=255)                                                                                                     gTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGAATGACATGGATGTAATGAACaa                             >  2:140950/1‑140 (MQ=255)                                                                                                      tttttttttGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGaa                                <  2:259538/136‑1 (MQ=255)                                                                                                      tttttttttGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGaa                                >  1:259538/1‑136 (MQ=255)                                                                                                       ttttttttGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAAt                                   >  2:794212/1‑132 (MQ=255)                                                                                                       ttttttttGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAAt                                   <  1:794212/132‑1 (MQ=255)                                                                                                            tttGAGTTTGATGATTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGaa                      <  2:106655/140‑1 (MQ=255)                                                                                                                     gatgatTTTTATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATgg             >  1:108750/1‑140 (MQ=255)                                                                                                                           ttttATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATg       <  2:327767/140‑1 (MQ=255)                                                                                                                           ttttATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATg       <  1:718675/140‑1 (MQ=255)                                                                                                                            tttATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGa      >  2:62162/1‑140 (MQ=255)                                                                                                                             ttATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAg     <  2:423326/140‑1 (MQ=255)                                                                                                                             ttATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAg     <  2:33681/140‑1 (MQ=255)                                                                                                                             ttATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAg     <  2:106538/140‑1 (MQ=255)                                                                                                                             ttATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAg     <  1:701641/140‑1 (MQ=255)                                                                                                                              tATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGc    <  2:1957/140‑1 (MQ=255)                                                                                                                              tATCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGc    <  2:156080/140‑1 (MQ=255)                                                                                                                               aTCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCa   >  2:494394/1‑140 (MQ=255)                                                                                                                               aTCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCa   <  1:81822/140‑1 (MQ=255)                                                                                                                                tCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCAt  <  2:58520/140‑1 (MQ=255)                                                                                                                                tCAAATCATGAC‑TTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCAt  <  1:718396/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                 GATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCAT  >  NC_000913/4556660‑4556927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑