Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,946,025	(A)7→6	intergenic (‑49/+90)	ruvA ← / ← yobI	Holliday junction branch migration complex subunit RuvA/uncharacterized protein YobI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,946,019	0	A	.	100.0%	241.9 / NA	57	intergenic (‑43/+96)	ruvA/yobI	Holliday junction branch migration complex subunit RuvA/uncharacterized protein YobI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (28/29);  total (28/29)

ATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTG  >  NC_000913/1945882‑1946154                                                                                                                                          |                                                                                                                                        aTCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaa                                                                                                                                       <  2:4408/140‑1 (MQ=255)   cGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaa                                                                                                                                     <  1:232342/140‑1 (MQ=255)      cATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatg                                                                                                                                  <  2:796204/140‑3 (MQ=255)      cATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatg                                                                                                                                  <  2:422508/140‑3 (MQ=255)        tatGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatgct                                                                                                                                >  2:32512/1‑136 (MQ=255)         atGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTg                                                                                                                              <  1:319359/140‑1 (MQ=255)           gCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGga                                                                                                                            <  2:248219/140‑1 (MQ=255)           gCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGga                                                                                                                            >  2:325800/1‑140 (MQ=255)                tCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGgatagat                                                                                                                       >  2:49911/1‑140 (MQ=255)                tCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGgatagat                                                                                                                       >  1:335587/1‑140 (MQ=255)                    aGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGgatag                                                                                                                         <  2:604869/134‑1 (MQ=255)                    aGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGgatag                                                                                                                         >  1:604869/1‑134 (MQ=255)                    aGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATcc                                                                                                                   >  2:499111/1‑140 (MQ=255)                    aGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATcc                                                                                                                   >  2:429029/1‑140 (MQ=255)                      ccTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAg                                                                                                                 >  2:475367/1‑140 (MQ=255)                         aCGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGa                                                                                                              <  1:473992/140‑1 (MQ=255)                         aCGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGa                                                                                                              >  2:697574/1‑140 (MQ=255)                               cccACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATg                                                                                                        <  1:475367/140‑1 (MQ=255)                                ccACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATga                                                                                                       >  2:505301/1‑140 (MQ=255)                                       aaTTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAAc                                                                                                <  1:505301/140‑1 (MQ=255)                                              acCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAg                                                                                         >  2:86868/1‑140 (MQ=255)                                                   cGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtc                                                                                    >  2:14097/1‑140 (MQ=255)                                                   cGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtc                                                                                    >  1:664675/1‑140 (MQ=255)                                                   cGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtc                                                                                    >  2:580562/1‑140 (MQ=255)                                                     ggggTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtctc                                                                                  >  1:711786/1‑140 (MQ=255)                                                      gggTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCg                                                                                 >  1:223142/1‑140 (MQ=255)                                                         ttgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATg                                                                              <  1:499111/140‑1 (MQ=255)                                                          tgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGAT‑AAGAAAACTTGCGAGGTGTCTCGATGaa                                                                            <  1:32512/140‑1 (MQ=255)                                                                tCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGaa                                                                       <  2:223142/140‑1 (MQ=255)                                                                      atgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                                                 <  2:648961/140‑1 (MQ=255)                                                                      atgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                                                 <  2:217896/140‑1 (MQ=255)                                                                       tgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGc                                                                <  1:325800/140‑1 (MQ=255)                                                                        gatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               <  2:711786/140‑1 (MQ=255)                                                                        gatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               <  1:86868/140‑1 (MQ=255)                                                                         atgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGcac                                                              >  1:343757/1‑140 (MQ=255)                                                                         atgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGcac                                                              >  1:34395/1‑140 (MQ=255)                                                                            ccTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGt                                                           <  1:697574/140‑1 (MQ=255)                                                                               ctGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtata                                                        <  2:801251/140‑1 (MQ=255)                                                                                  aGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAAtt                                                     <  1:573032/140‑1 (MQ=255)                                                                                    tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               <  2:651072/128‑1 (MQ=255)                                                                                    tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               >  1:525768/1‑128 (MQ=255)                                                                                    tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               <  2:525768/128‑1 (MQ=255)                                                                                    tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGca                                                               >  1:651072/1‑128 (MQ=255)                                                                                      tGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTAtt                                                 >  2:85978/1‑140 (MQ=255)                                                                                        ccTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                                                 <  1:728966/122‑1 (MQ=255)                                                                                        ccTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                                                                 >  2:728966/1‑122 (MQ=255)                                                                                        ccTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCt                                               >  2:356682/1‑140 (MQ=255)                                                                                        ccTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCt                                               <  1:536991/140‑1 (MQ=255)                                                                                         cTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTa                                              <  2:451544/140‑1 (MQ=255)                                                                                          tATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTAc                                             <  2:343757/140‑1 (MQ=255)                                                                                            tCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAg                                           >  1:109384/1‑140 (MQ=255)                                                                                                       tCCTGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGa                                >  2:697704/1‑140 (MQ=255)                                                                                                          tGCGTAATGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGc                             >  2:671910/1‑140 (MQ=255)                                                                                                                 tGAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATAtt                      >  2:390175/1‑140 (MQ=255)                                                                                                                  gAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATAttt                     <  1:671910/140‑1 (MQ=255)                                                                                                                  gAATCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATAttt                     <  2:48476/140‑1 (MQ=255)                                                                                                                    aTCAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTa                   <  1:356682/140‑1 (MQ=255)                                                                                                                      cAAAGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAat                 <  2:173029/140‑1 (MQ=255)                                                                                                                        aaGATAATGCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAatat               <  2:335587/140‑1 (MQ=255)                                                                                                                                gCTGTATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATAtt       <  2:271881/140‑1 (MQ=255)                                                                                                                                    tATGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGt   >  1:324209/1‑140 (MQ=255)                                                                                                                                     aTGAT‑AAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTg  >  2:214313/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        ATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGGTTATATTGGAAGCAAATATTTTAATATTACATATTCAGTG  >  NC_000913/1945882‑1946154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑