Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,405,158	(A)8→7	intergenic (‑86/+545)	nuoA ← / ← lrhA	NADH:quinone oxidoreductase subunit A/DNA‑binding transcriptional dual regulator LrhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,405,151	0	A	.	100.0%	259.1 / NA	61	intergenic (‑79/+552)	nuoA/lrhA	NADH:quinone oxidoreductase subunit A/DNA‑binding transcriptional dual regulator LrhA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (31/30);  total (31/30)

ATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGC  >  NC_000913/2405020‑2405286                                                                                                                                    |                                                                                                                                        aTAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATaaaaaaacc                                                                                                                                 >  2:800945/1‑138 (MQ=255)        aTGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAAt                                                                                                                         <  1:441789/140‑1 (MQ=255)          gCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCa                                                                                                                       <  1:154816/140‑1 (MQ=255)               gTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTa                                                                                                                  >  1:625212/1‑140 (MQ=255)                   tgaGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGaaa                                                                                                              >  2:342693/1‑140 (MQ=255)                   tgaGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGaaa                                                                                                              <  2:351119/140‑1 (MQ=255)                     aGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAAtt                                                                                                            >  2:460744/1‑140 (MQ=255)                     aGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAAtt                                                                                                            >  1:42498/1‑140 (MQ=255)                          tGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCtttt                                                                                                       >  1:354179/1‑140 (MQ=255)                             cTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTtata                                                                                                    <  2:383470/140‑1 (MQ=255)                               tCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACa                                                                                                  <  1:439831/140‑1 (MQ=255)                               tCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACa                                                                                                  <  2:42498/140‑1 (MQ=255)                                 aGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGc                                                                                                >  2:250989/1‑140 (MQ=255)                                    ggATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTaa                                                                                             <  1:800945/140‑1 (MQ=255)                                    ggATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTaa                                                                                             <  2:257934/140‑1 (MQ=255)                                                   cATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGTTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGtttttt                                                                              >  1:718510/1‑140 (MQ=255)                                                    aTTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTa                                                                             >  1:345324/1‑140 (MQ=255)                                                    aTTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTa                                                                             >  2:189441/1‑140 (MQ=255)                                                    aTTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTa                                                                             <  1:240511/140‑1 (MQ=255)                                                      tGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACt                                                                           >  2:424965/1‑140 (MQ=255)                                                        cTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTcc                                                                         <  1:342693/140‑1 (MQ=255)                                                        cTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTcc                                                                         <  1:250989/140‑1 (MQ=255)                                                         ttACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCt                                                                        <  2:345324/140‑1 (MQ=255)                                                           aCTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTcc                                                                         <  1:209506/137‑1 (MQ=255)                                                           aCTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTcc                                                                         >  2:209506/1‑137 (MQ=255)                                                            cTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTc                                                                     >  1:173393/1‑140 (MQ=255)                                                            cTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTc                                                                     >  2:488228/1‑140 (MQ=255)                                                              catcaAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCta                                                                   >  1:654935/1‑140 (MQ=255)                                                              catcaAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCta                                                                   >  2:252508/1‑140 (MQ=255)                                                               atcaAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCtat                                                                  <  2:354179/140‑1 (MQ=255)                                                                tcaAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCtata                                                                 >  1:143984/1‑140 (MQ=255)                                                                  aaaaGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAAc                                                               >  2:647985/1‑140 (MQ=255)                                                                       tAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCtttt                                                          >  1:750032/1‑140 (MQ=255)                                                                         gcgcCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTcc                                                                         <  2:693969/123‑1 (MQ=255)                                                                         gcgcCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTcc                                                                         >  1:693969/1‑123 (MQ=255)                                                                                      tGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACaaa                                           >  1:106665/1‑140 (MQ=255)                                                                                          ctTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAAC‑TTAACAAAAGttt                                      >  2:776256/1‑140 (MQ=255)                                                                                           ttCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGttt                                      >  2:419424/1‑140 (MQ=255)                                                                                             cACGGCAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTttct                                    >  2:158157/1‑140 (MQ=255)                                                                                             cACGGCAGAAGGACCCCACAACGATTTATTGGGAAGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATTCAGCTAATTGATGTGGTTTTTTACCCCTTTCTATAACCCTTTGTCAACCTTAACAAAAGTttct                                    <  2:143984/140‑1 (MQ=255)                                                                                                  cAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTcaca                               <  2:44077/140‑1 (MQ=255)                                                                                                  cAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTcaca                               <  2:566976/140‑1 (MQ=255)                                                                                                  cAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTcaca                               >  1:566976/1‑140 (MQ=255)                                                                                                  cAGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTcaca                               <  1:647985/140‑1 (MQ=255)                                                                                                   aGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACAt                              <  2:654935/140‑1 (MQ=255)                                                                                                   aGATGGACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACAt                              <  2:106665/140‑1 (MQ=255)                                                                                                       ggACGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGt                          >  1:569862/1‑140 (MQ=255)                                                                                                          cGCCACATCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTa                       >  2:226366/1‑140 (MQ=255)                                                                                                             cacaTCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACCGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACat                    <  2:569862/140‑1 (MQ=255)                                                                                                              acaTCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACata                   <  2:173393/140‑1 (MQ=255)                                                                                                               caTCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTttctt                                   >  2:478123/1‑123 (MQ=255)                                                                                                               caTCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTttctt                                   <  1:478123/123‑1 (MQ=255)                                                                                                               caTCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACataa                  <  1:419424/140‑1 (MQ=255)                                                                                                               caTCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACataa                  >  2:245872/1‑140 (MQ=255)                                                                                                                aTCGATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACataat                 <  1:570109/140‑1 (MQ=255)                                                                                                                   gATTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATc              >  2:552805/1‑140 (MQ=255)                                                                                                                    aTTCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCa             <  1:245872/140‑1 (MQ=255)                                                                                                                      tCATGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAac           <  2:135749/140‑1 (MQ=255)                                                                                                                        aTGGGGAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAacac         <  1:252508/140‑1 (MQ=255)                                                                                                                          ggggAGGAAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCa       <  1:158157/140‑1 (MQ=255)                                                                                                                              aggaAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAg   >  2:321053/1‑140 (MQ=255)                                                                                                                              aggaAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAg   <  2:113050/140‑1 (MQ=255)                                                                                                                               ggaAT‑AAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGc  >  2:399310/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        ATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGC  >  NC_000913/2405020‑2405286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑