Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,639,879	(T)8→7	intergenic (‑159/‑232)	uspB ← / → uspA	putative universal stress (ethanol tolerance) protein B/universal stress global stress response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,639,872	0	T	.	100.0%	264.0 / NA	62	intergenic (‑152/‑239)	uspB/uspA	putative universal stress (ethanol tolerance) protein B/universal stress global stress response regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (30/32);  total (30/32)

ACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGG  >  NC_000913/3639736‑3640007                                                                                                                                         |                                                                                                                                        aCCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttt                                                                                                                                      >  1:84206/1‑140 (MQ=255) aCCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttt                                                                                                                                      >  1:426344/1‑140 (MQ=255)  ccTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGttttt                                                                                                                                     <  2:260891/140‑1 (MQ=255)       ccGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca                                                                                                                                <  2:394024/140‑4 (MQ=255)       ccGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca                                                                                                                                <  2:29330/140‑4 (MQ=255)             aGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTTTAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGt                                                                                                                         <  2:807372/140‑1 (MQ=255)                tCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTaa                                                                                                                      <  2:426344/140‑1 (MQ=255)                 ccACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAAc                                                                                                                     <  1:565146/140‑1 (MQ=255)                 ccACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAAc                                                                                                                     <  2:84206/140‑1 (MQ=255)                   aCCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCa                                                                                                                   <  1:488798/140‑1 (MQ=255)                     cccGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACt                                                                                                                 <  2:571638/140‑1 (MQ=255)                     cccGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACt                                                                                                                 <  1:256779/140‑1 (MQ=255)                     cccGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACt                                                                                                                 <  1:234982/140‑1 (MQ=255)                         gggCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTa                                                                                                                >  2:501888/1‑137 (MQ=255)                         gggCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTa                                                                                                                <  1:501888/137‑1 (MQ=255)                               ccGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAatatat                                                                                                       >  2:443961/1‑140 (MQ=255)                                                          aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACg                                                                            >  2:26707/1‑140 (MQ=255)                                                               cAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTaa                                                                       >  2:88483/1‑140 (MQ=255)                                                                  gTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCa                                                                    >  1:206950/1‑140 (MQ=255)                                                                     cTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAgg                                                                 >  1:56454/1‑140 (MQ=255)                                                                       ggAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAgg                                                                 <  2:743876/138‑1 (MQ=255)                                                                       ggAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAgg                                                                 >  1:743876/1‑138 (MQ=255)                                                                       ggAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCg                                                               >  2:806959/1‑140 (MQ=255)                                                                       ggAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCg                                                               >  1:140377/1‑140 (MQ=255)                                                                       ggAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCg                                                               <  1:88483/140‑1 (MQ=255)                                                                         agaAACCGCTTTTGTGGTGACCAACATACGAGCGACTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGa                                                             <  2:135493/140‑1 (MQ=255)                                                                           aaaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGAtt                                                           <  1:535088/140‑1 (MQ=255)                                                                            aaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTg                                                          >  1:757473/1‑140 (MQ=255)                                                                             aCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGa                                                         <  2:327717/140‑1 (MQ=255)                                                                                gCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGATgg                                                      <  1:622639/140‑1 (MQ=255)                                                                                      gtggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGatcatc                                                <  2:206950/140‑1 (MQ=255)                                                                                       tggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATcc                                               >  2:281444/1‑140 (MQ=255)                                                                                          tgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCggg                                            <  1:681101/140‑1 (MQ=255)                                                                                          tgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCggg                                            <  1:281444/140‑1 (MQ=255)                                                                                          tgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCggg                                            <  1:654638/140‑1 (MQ=255)                                                                                          tgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCggg                                            <  1:646180/140‑1 (MQ=255)                                                                                           gACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                           <  2:351922/140‑1 (MQ=255)                                                                                            aCCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTc                                          >  1:29479/1‑140 (MQ=255)                                                                                             ccAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCg                                         <  1:827167/140‑1 (MQ=255)                                                                                              cAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                           <  1:569110/137‑1 (MQ=255)                                                                                              cAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                           >  2:569110/1‑137 (MQ=255)                                                                                               aaCATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCt                                       <  2:140377/140‑1 (MQ=255)                                                                                                aCATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCta                                      <  2:743030/140‑1 (MQ=255)                                                                                                 cATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCtat                                     <  2:714420/140‑1 (MQ=255)                                                                                                           gCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGa                           >  1:331698/1‑140 (MQ=255)                                                                                                           gCTCTATAGATAGTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGa                           >  1:778711/1‑140 (MQ=255)                                                                                                                  agatagTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCtt                    <  2:29479/140‑1 (MQ=255)                                                                                                                   gatagTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTa                   <  1:806959/140‑1 (MQ=255)                                                                                                                      agTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAaca                >  1:158400/1‑140 (MQ=255)                                                                                                                      agTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAaca                <  2:605331/140‑1 (MQ=255)                                                                                                                      agTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAaca                >  1:43057/1‑140 (MQ=255)                                                                                                                      agTGTAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAaca                >  1:605331/1‑140 (MQ=255)                                                                                                                        tgtAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACt              >  2:541451/1‑140 (MQ=255)                                                                                                                         gtAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAaca                >  1:642539/1‑137 (MQ=255)                                                                                                                         gtAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAaca                <  2:642539/137‑1 (MQ=255)                                                                                                                         gtAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTg             >  2:637112/1‑140 (MQ=255)                                                                                                                         gtAGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTg             >  2:293737/1‑140 (MQ=255)                                                                                                                           aGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGaa           >  2:215698/1‑140 (MQ=255)                                                                                                                           aGGAGATCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGaa           >  2:774156/1‑140 (MQ=255)                                                                                                                              agaTCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGAt                          >  2:725709/1‑122 (MQ=255)                                                                                                                              agaTCAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGAt                          <  1:725709/122‑1 (MQ=255)                                                                                                                                  cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTAc    >  2:348297/1‑140 (MQ=255)                                                                                                                                  cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTAc    >  2:653410/1‑140 (MQ=255)                                                                                                                                  cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTAc    >  1:388106/1‑140 (MQ=255)                                                                                                                                  cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTAc    >  1:268724/1‑140 (MQ=255)                                                                                                                                  cAGGTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTAc    >  1:517837/1‑140 (MQ=255)                                                                                                                                    ggTTG‑TTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACgg  <  1:774156/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ACCTGCCCGCGGAGTTCCACCCCGGGGCTACCGCTCCCGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGG  >  NC_000913/3639736‑3640007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑