| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,177,509 | (C)6→7 | coding (402/630 nt) | nudF ← | ADP‑sugar pyrophosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,177,503 | 1 | . | C | 96.3% | 91.3 / ‑2.9 | 27 | coding (408/630 nt) | nudF | ADP‑sugar pyrophosphatase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (20/6); total (20/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
CCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAG > NC_000913/3177364‑3177637 | ccTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑g < 1:291614/140‑1 (MQ=255) cACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑cccccccgggc < 2:421074/140‑6 (MQ=255) tATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCa < 2:70866/140‑1 (MQ=255) tGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAgg < 1:169175/140‑1 (MQ=255) cTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGaaa > 1:688013/1‑140 (MQ=255) ttCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACtt > 1:97247/1‑140 (MQ=255) tCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTa > 1:140674/1‑140 (MQ=255) tCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTa > 1:700133/1‑140 (MQ=255) ggCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAacac > 1:160108/1‑140 (MQ=255) gAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCg > 2:319074/1‑140 (MQ=255) cgcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGttt > 2:466441/1‑140 (MQ=255) cgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGtt > 1:632593/1‑137 (MQ=255) cgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGtt < 2:632593/137‑1 (MQ=255) atatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTg > 1:772245/1‑136 (MQ=255) atatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTg < 2:608288/136‑1 (MQ=255) atatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTg > 1:608288/1‑136 (MQ=255) atatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTg < 2:772245/136‑1 (MQ=255) ttttCACCACCCAGACCGTAAATCCCGCTGGGGGTGGTGGCGCCCCCTCCCCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACCCCGTTTTGGTCCGTTTGACTATCAGTCCCg < 2:688013/140‑1 (MQ=255) ccGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACggg > 2:258481/1‑140 (MQ=255) cGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGc > 1:359146/1‑140 (MQ=255) ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc > 1:315721/1‑140 (MQ=255) ttGCGGTCGTGGCGCCCCCTCCCCCCACCATAATTGACGAACGCTCCCTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc < 2:315721/140‑1 (MQ=255) tGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACa > 2:204131/1‑140 (MQ=255) ccccgcTTCCCCCACAATAATTGCCGAACGCTCTTGGGTCC‑CCCCCCGGGCTT‑GCAAGGAAACTAACCCCCGTTTTGGCCCGTTTGCCTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACtt < 2:359146/135‑1 (MQ=255) cACTCCCCCCCCCATAATTGACGAACGCTCACTGGTCC‑CCCCCCGGGCTT‑GCCAGAAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCa < 2:160108/140‑1 (MQ=255) agctcccccccccATAATTGACGACCGCTCACTGGTCC‑CCCCCCGGGTTT‑GCCAGAAACCTTAACACCGGTTTGGTCCGTTTGACTACCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCa < 2:712827/133‑1 (MQ=255) cctcccccccccAAAATTGACGACCGCTCCCTGGTCC‑CCCCCCGGGCTT‑GCCAGAAAACTTACCACCGGTTGGGTCGTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCAc < 2:140674/134‑1 (MQ=255) aTAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatct > 1:712827/1‑139 (MQ=255) aTAATTGACGAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatca > 1:388493/1‑140 (MQ=255) tAATTAACAAACGCTCACTGGT‑C‑CCCCCCCGGCTTCGCCAGAAAACTTACCACCGTTTTGTCCCGTTTGGCTATCATTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatcat < 2:780482/140‑1 (MQ=255) cGACCGCTCACGGGTCC‑CCCCCCGGGCTT‑GCCAGGAAACTTACCCCCGGTTTGGTCCGTTTGCCTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCa < 2:97247/140‑1 (MQ=255) gAACGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa > 1:470813/1‑140 (MQ=255) cGCTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCa > 2:644765/1‑140 (MQ=255) cccgaCTGGC‑G‑CCCCCCCGGTTTGGCCAGGAAACTTAACGCCGTTTTGGTCCGTTTGACTACCTGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCAt < 2:700133/136‑1 (MQ=255) cTCACTGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCAtc > 1:679574/1‑140 (MQ=255) tGGT‑GCCCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAg > 2:703421/1‑140 (MQ=255) | CCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCCCGGGCTT‑GCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAG > NC_000913/3177364‑3177637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |