| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,490,764 | (C)5→6 | coding (66/564 nt) | pabA ← | aminodeoxychorismate synthase subunit 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,490,759 | 1 | . | C | 95.0% | 139.7 / ‑0.0 | 40 | coding (71/564 nt) | pabA | aminodeoxychorismate synthase subunit 2 |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (20/18); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATAACGTCAAGGGAGATCCCGGCTTCATCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCG‑C‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACCGCGCTATTTTACTCAGATTCCCCGGCTTCGCTTACCAC > NC_000913/3490632‑3490890 | aaTAACGTCAAGGGAGATCCCGGCTTCATCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTca > 1:523417/1‑140 (MQ=255) aCGTCAAGGGAGATCCCGGCTTCATCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACaaa < 2:762889/140‑1 (MQ=255) tCAAGGGAGATCCCGGCTTCATCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGt < 2:611416/140‑1 (MQ=255) tCAAGGGAGATCCCGGCTTCATCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGt < 1:95202/140‑1 (MQ=255) gCTTCATCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTcc < 1:363649/140‑1 (MQ=255) cTTCATCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCa > 1:156751/1‑140 (MQ=255) cTTCATCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCG‑C‑CCCCAGGTCACAAAAGGACTTGTAGAGGGTCCAg > 2:523519/1‑140 (MQ=255) aTCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTag > 1:294654/1‑128 (MQ=255) aTCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTag < 2:294654/128‑1 (MQ=255) cGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGaa < 1:11311/140‑1 (MQ=255) cGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGaa > 2:11311/1‑140 (MQ=255) cGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGaa < 1:514404/140‑1 (MQ=255) gTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCAGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAAt < 1:104658/140‑1 (MQ=255) tACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATc > 2:451203/1‑140 (MQ=255) gggCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGt < 2:430484/140‑1 (MQ=255) ggCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGtt < 1:620393/140‑1 (MQ=255) gCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTa > 1:564556/1‑140 (MQ=255) gCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTa > 2:621510/1‑140 (MQ=255) gCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTa > 2:417524/1‑140 (MQ=255) aGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCt < 1:278714/140‑1 (MQ=255) aTGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCa > 1:607055/1‑140 (MQ=255) aTGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCa > 2:626740/1‑140 (MQ=255) aTGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCa > 1:808874/1‑140 (MQ=255) aaTTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGc > 1:396283/1‑134 (MQ=255) aaTTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGc < 2:396283/134‑1 (MQ=255) aaTTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATc > 2:32709/1‑140 (MQ=255) ttttGTGGTTTAAGGGCGTCGATATCCGCCAGCGTAAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGGTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATgg > 2:545279/1‑140 (MQ=255) ggTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTc > 2:343450/1‑140 (MQ=255) tCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGa > 1:331906/1‑140 (MQ=255) tatCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACcg < 2:564556/140‑1 (MQ=255) tCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACcgcg < 2:523417/140‑1 (MQ=255) cAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACCGCGCTAtt < 1:451203/140‑1 (MQ=255) cGTCAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGG‑GGCTCCGGTACAAAAGAACCGCGCTATTTTAc > 1:752841/1‑140 (MQ=255) cAACGCATCGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACCGCGCTATTTTACTc < 1:621510/140‑1 (MQ=255) cGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACCGCGCTATTTTACTCAGATTccc > 1:395474/1‑140 (MQ=255) cGTTGCGCTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACCGCGCTATTTTACTCAGATTccc > 1:216402/1‑140 (MQ=255) cgcTTAACCAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACCGCGCTATTTTACTCAGATTCCCCGGCt < 2:156751/140‑1 (MQ=255) ccAGCACATCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACCGCGCTATTTTACTCAGATTCCCCGGCTTCGCTTa < 1:32709/140‑1 (MQ=255) gCACATCCG‑CTCCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACCGCGCTATTTTACTCAGATTCCCCGGCTTCGCTTACCa < 1:772775/140‑1 (MQ=255) cacaTCCGCC‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACCGCGCTATTTTACTCAGATTCCCCGGCTTCGCTTACCAc > 2:337907/1‑140 (MQ=255) | AATAACGTCAAGGGAGATCCCGGCTTCATCTGGCGTACAGGGGCCAGGTGAGATGACAATTTTTTGTGGTTTAAGGGCGTCGATATCCGCCAGCGTCAACGCATCGTTGCGCTTAACCAGCACATCCG‑C‑CCCCAGTTCACAAAAGTACTGGTAGAGGTTCCAGGTAAAAGAATCGTAGTTATCTATAAGCAGGATCATGGCGGCTCCGGTACAAAAGAACCGCGCTATTTTACTCAGATTCCCCGGCTTCGCTTACCAC > NC_000913/3490632‑3490890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |