Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,060,843	(G)6→7	coding (397/711 nt)	yihL →	putative transcriptional regulator YihL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,060,837	1	.	G	100.0%	158.2 / NA	43	W131G (TGG→GGG)	yihL	putative transcriptional regulator YihL
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/21);  total (22/21)

CAGCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGAC  >  NC_000913/4060702‑4060970                                                                                                                                         |                                                                                                                                      cAGCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑gggg                                                                                                                                   <  1:543713/140‑1 (MQ=255)   gCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑gggggg                                                                                                                                 <  1:549283/140‑1 (MQ=255)    cGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑ggggggg                                                                                                                                <  2:52584/140‑2 (MQ=255)      ggCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑gggggggcg                                                                                                                              >  2:644413/1‑137 (MQ=255)           cAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGa                                                                                                                          <  1:154034/140‑1 (MQ=255)            aaTTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGaa                                                                                                                         <  1:644413/140‑1 (MQ=255)                 aGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACa                                                                                                                    >  1:328137/1‑140 (MQ=255)                      gggCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAg                                                                                                               >  1:127902/1‑140 (MQ=255)                      gggCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAg                                                                                                               >  1:287020/1‑140 (MQ=255)                          gagaGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGtttt                                                                                                           <  1:3661/140‑1 (MQ=255)                            gagCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCt                                                                                                         >  1:63334/1‑140 (MQ=255)                             agCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTa                                                                                                        >  1:319801/1‑140 (MQ=255)                              gCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTAt                                                                                                       <  2:127902/140‑1 (MQ=255)                                cTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCa                                                                                                     <  1:185602/140‑1 (MQ=255)                                cTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCa                                                                                                     <  1:62102/140‑1 (MQ=255)                                cTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCa                                                                                                     <  1:215515/140‑1 (MQ=255)                                  tCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACg                                                                                                   <  2:690889/140‑1 (MQ=255)                                  tCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACg                                                                                                   <  1:694639/140‑1 (MQ=255)                                  tCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACg                                                                                                   >  1:690889/1‑140 (MQ=255)                                  tCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACg                                                                                                   >  1:497048/1‑140 (MQ=255)                                   ccTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGa                                                                                                  <  2:287020/140‑1 (MQ=255)                                   ccTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGa                                                                                                  <  2:328137/140‑1 (MQ=255)                                              aCCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGa                                                                                                  >  2:296849/1‑129 (MQ=255)                                              aCCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGa                                                                                                  <  1:296849/129‑1 (MQ=255)                                              aCCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACatatat                                                                                         >  2:702333/1‑138 (MQ=255)                                              aCCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACatatat                                                                                         <  1:702333/138‑1 (MQ=255)                                                    aaaaaCCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTg                                                                                 >  1:442603/1‑140 (MQ=255)                                                             aCCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTc                                                                        <  1:132724/140‑1 (MQ=255)                                                                 gCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCggg                                                                    <  2:808327/140‑1 (MQ=255)                                                                 gCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCggg                                                                    <  2:319801/140‑1 (MQ=255)                                                                             agaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaaca                                                        <  2:63334/140‑1 (MQ=255)                                                                             agaCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaaca                                                        >  1:326082/1‑140 (MQ=255)                                                                               aCGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGaacaac                                                      >  1:266187/1‑140 (MQ=255)                                                                                                     tGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGc                                >  1:247427/1‑140 (MQ=255)                                                                                                       aCGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAg                              >  1:721338/1‑140 (MQ=255)                                                                                                       aCGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAg                              <  2:266187/140‑1 (MQ=255)                                                                                                       aCGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAg                              >  1:812684/1‑140 (MQ=255)                                                                                                        cGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAAGATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGt                             >  2:720316/1‑140 (MQ=255)                                                                                                        cGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGt                             >  2:674184/1‑140 (MQ=255)                                                                                                                     aCTCTATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGc                <  2:762168/140‑1 (MQ=255)                                                                                                                         tATCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTAcc            >  2:445299/1‑140 (MQ=255)                                                                                                                          aTCGCATCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCa           >  1:634347/1‑140 (MQ=255)                                                                                                                                tCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCa           >  2:146057/1‑134 (MQ=255)                                                                                                                                tCACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCa           <  1:146057/134‑1 (MQ=255)                                                                                                                                 cACCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAagag    >  1:296145/1‑140 (MQ=255)                                                                                                                                  aCCGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAgaga   <  1:178477/140‑1 (MQ=255)                                                                                                                                   ccGGCTGGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGAc  >  2:480427/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CAGCGGGCCGCAATTGAGCAAGGGCGAGAGCCTTCCTGGGGGTTTACCGAGAAAAACCGTACCAGCGATATTCCCGAGACGCTCGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCT‑GGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGAC  >  NC_000913/4060702‑4060970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑