Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	630,889	C→T	T332T (ACC→ACT)	cstA →	carbon starvation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	630,889	0	C	T	100.0%	98.0 / NA	28	T332T (ACC→ACT)	cstA	carbon starvation protein A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/10);  total (18/10)

ATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCG  >  NC_000913/630755‑631018                                                                                                                                       |                                                                                                                                  aTCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGc                                                                                                                              <  1:419649/140‑1 (MQ=255)                                    gATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtct                                                                                           <  2:825417/140‑1 (MQ=255)                                           cgcCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                                                                                    >  2:435858/1‑140 (MQ=255)                                               cGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAcc                                                                                <  1:551778/140‑1 (MQ=255)                                                            gCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc                                                                   >  2:69720/1‑140 (MQ=255)                                                            gCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc                                                                   >  2:244466/1‑140 (MQ=255)                                                            gCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGc                                                                   >  1:685913/1‑140 (MQ=255)                                                                   cTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaa                                                            >  1:368635/1‑140 (MQ=255)                                                                      aCCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga                                                         >  1:257089/1‑140 (MQ=255)                                                                      aCCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga                                                         >  1:799380/1‑140 (MQ=255)                                                                      aCCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga                                                         >  1:431063/1‑140 (MQ=255)                                                                         aaaTTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAgg                                                      <  2:257089/140‑1 (MQ=255)                                                                                  gATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTg                                             <  2:368635/140‑1 (MQ=255)                                                                                     ggCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                                          >  2:638803/1‑140 (MQ=255)                                                                                            gCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGc                                   <  2:74088/140‑1 (MQ=255)                                                                                            gCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGc                                   >  2:196223/1‑140 (MQ=255)                                                                                               cGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTAc                                <  1:102704/140‑1 (MQ=255)                                                                                                 gTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCt                                           >  2:431471/1‑127 (MQ=255)                                                                                                 gTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCt                                           <  1:431471/127‑1 (MQ=255)                                                                                                 gTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCgg                                    >  1:789260/1‑134 (MQ=255)                                                                                                 gTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCgg                                    <  2:789260/134‑1 (MQ=255)                                                                                                 gTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACgg                              >  2:796005/1‑140 (MQ=255)                                                                                                    tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                           <  2:431063/140‑1 (MQ=255)                                                                                                      gACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa                         >  1:636374/1‑140 (MQ=255)                                                                                                      gACCGGTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGa                         >  1:442498/1‑140 (MQ=255)                                                                                                           gTAACCTGTTCCCGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                    >  2:488530/1‑140 (MQ=255)                                                                                                                     cccGTTCCTGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCt          >  1:787569/1‑140 (MQ=255)                                                                                                                             tGTTTATCACTATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGATCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCg  >  1:427726/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                  ATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCG  >  NC_000913/630755‑631018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑