Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,074,228	A→G	T164A (ACG→GCG)	yihV →	6‑deoxy‑6‑sulfofructose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,074,228	0	A	G	100.0%	188.0 / NA	51	T164A (ACG→GCG)	yihV	6‑deoxy‑6‑sulfofructose kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/28);  total (23/28)

CGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGT  >  NC_000913/4074092‑4074359                                                                                                                                         |                                                                                                                                    cGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTgcgc                                                                                                                                  <  1:825835/140‑1 (MQ=255) cGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTgcgc                                                                                                                                  <  1:58631/140‑1 (MQ=255)   cAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGcgccg                                                                                                                                <  2:500687/140‑1 (MQ=255)        tGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGa                                                                                                                           <  1:298066/140‑1 (MQ=255)          gTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGata                                                                                                                         >  1:272535/1‑140 (MQ=255)          gTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGata                                                                                                                         <  2:616402/140‑1 (MQ=255)           ttGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGatat                                                                                                                        >  2:87306/1‑140 (MQ=255)               aggaAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGt                                                                                                                    <  1:142238/140‑1 (MQ=255)               aggaAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGt                                                                                                                    <  2:331164/140‑1 (MQ=255)                ggaAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTg                                                                                                                   >  1:127156/1‑140 (MQ=255)                  aaaTTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAg                                                                                                                 <  2:549527/140‑1 (MQ=255)                  aaaTTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAg                                                                                                                 >  2:643413/1‑140 (MQ=255)                  aaaTTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAg                                                                                                                 >  2:311920/1‑140 (MQ=255)                     ttGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCtg                                                                                                              <  2:127156/140‑1 (MQ=255)                     ttGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCtg                                                                                                              <  1:213438/140‑1 (MQ=255)                      tGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTATGCTTGCACGACGGCGCTCAAATAGCACTCCCGCTCTCCCGTTCGGCGGGTCTGTTGATCATCCTGCACCGGAACAGTACGTCGCACGGTCTCAGGGAGCtgg                                                                                                             <  2:588136/140‑1 (MQ=255)                                       aTGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCAc                                                                                            <  2:474451/140‑1 (MQ=255)                                       aTGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCAc                                                                                            >  1:363373/1‑140 (MQ=255)                                        tgttgtGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCg                                                                                                 >  1:442040/1‑134 (MQ=255)                                        tgttgtGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCg                                                                                                 <  2:442040/134‑1 (MQ=255)                                         gttgtGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCAcgc                                                                                          <  2:438424/140‑1 (MQ=255)                                            gtgCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGc                                                                                       <  1:193160/140‑1 (MQ=255)                                             tgCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGcc                                                                                      >  1:594322/1‑140 (MQ=255)                                               cTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCtt                                                                                    >  2:241616/1‑140 (MQ=255)                                                    aGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAg                                                                               >  2:164236/1‑140 (MQ=255)                                                       tGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAAc                                                                            >  1:707966/1‑140 (MQ=255)                                                       tGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAAc                                                                            <  2:640793/140‑1 (MQ=255)                                                                gCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTgg                                                                   <  1:92269/140‑1 (MQ=255)                                                                 cACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGgc                                                                  >  1:93323/1‑140 (MQ=255)                                                                 cACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGgc                                                                  >  1:671474/1‑140 (MQ=255)                                                                     acgGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGgcgcgc                                                              >  2:464488/1‑140 (MQ=255)                                                                            tAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACgg                                                       <  2:594322/140‑1 (MQ=255)                                                                                  aGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGa                                                 <  1:464488/140‑1 (MQ=255)                                                                                  aGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGa                                                 <  1:311920/140‑1 (MQ=255)                                                                                    ccTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGaaa                                               <  1:674409/140‑1 (MQ=255)                                                                                       tCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAagag                                            <  1:87306/140‑1 (MQ=255)                                                                                        cACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCGCGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAAc                                                         <  2:279514/126‑1 (MQ=255)                                                                                        cACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAAc                                                         >  1:279514/1‑126 (MQ=255)                                                                                            cTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGc                                       <  2:606000/140‑1 (MQ=255)                                                                                             tGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGcc                                      >  2:673665/1‑140 (MQ=255)                                                                                              ggCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCa                                     >  2:62228/1‑140 (MQ=255)                                                                                               gCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAg                                    <  1:62228/140‑1 (MQ=255)                                                                                               gCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAg                                    <  1:463249/140‑1 (MQ=255)                                                                                                  cGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTgc                                 <  2:272535/140‑1 (MQ=255)                                                                                                    tCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTgcgc                               >  1:775647/1‑140 (MQ=255)                                                                                                     cAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCt                              >  2:683530/1‑140 (MQ=255)                                                                                                        gCGGGTGTGATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTaaa                           <  2:93323/140‑1 (MQ=255)                                                                                                             tgtgATGACCGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAgg                      <  2:707966/140‑1 (MQ=255)                                                                                                                     ccGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACg              <  1:673665/140‑1 (MQ=255)                                                                                                                      cGTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGc             >  1:228116/1‑140 (MQ=255)                                                                                                                       gTTCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCt            >  2:279076/1‑140 (MQ=255)                                                                                                                         tCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTca          >  1:429363/1‑140 (MQ=255)                                                                                                                         tCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTca          >  2:337242/1‑140 (MQ=255)                                                                                                                         tCTGGACGGGGACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTca          <  1:740174/140‑1 (MQ=255)                                                                                                                                 gggACATTGCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGt  <  1:820633/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGT  >  NC_000913/4074092‑4074359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑