Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,274,165	A→G	N14S (AAT→AGT)	ssb →	ssDNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,274,165	0	A	G	100.0%	181.8 / NA	50	N14S (AAT→AGT)	ssb	ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/28);  total (22/28)

CGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGT  >  NC_000913/4274029‑4274297                                                                                                                                         |                                                                                                                                     cGGGACCGAGGTCACAACATAGTAAAAGCGGTATTGGTAATGATACAATCGCGAGTTTACACTTCTTCAGAACGAGTTTTTTCAGGAGACACGATTATGGACAGCAGAGGCGTAAACACGGTTACTCCCGTTGGTAGTCt                                                                                                                                   <  2:198389/140‑1 (MQ=255)     aCCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGt                                                                                                                               <  2:821255/140‑1 (MQ=255)       cGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCa                                                                                                                             <  2:494304/140‑1 (MQ=255)             cacaACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGAccc                                                                                                                       <  1:560014/140‑1 (MQ=255)               caacaTAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCgg                                                                                                                     <  1:458617/140‑1 (MQ=255)                 acaTAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGaa                                                                                                                   <  2:554457/140‑1 (MQ=255)                    tAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTa                                                                                                                <  2:759965/140‑1 (MQ=255)                    tAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTa                                                                                                                <  1:372070/140‑1 (MQ=255)                     aGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTAc                                                                                                               >  2:211156/1‑140 (MQ=255)                      gTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCgg                                                                                                                     >  2:436890/1‑133 (MQ=255)                      gTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCgg                                                                                                                     <  1:436890/133‑1 (MQ=255)                        aaaaGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCt                                                                                                            <  2:102777/140‑1 (MQ=255)                         aaaGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTa                                                                                                           <  1:548648/140‑1 (MQ=255)                          aaGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTAc                                                                                                          <  2:619787/140‑1 (MQ=255)                               cTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGcc                                                                                                     >  2:661268/1‑140 (MQ=255)                                  ttGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCaaa                                                                                                  <  2:622111/140‑1 (MQ=255)                                            tACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCaaa                                                                                                  <  2:628153/130‑1 (MQ=255)                                            tACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCaaa                                                                                                  >  1:628153/1‑130 (MQ=255)                                            tACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCa                                                                                        >  2:467828/1‑140 (MQ=255)                                            tACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCa                                                                                        >  2:597760/1‑140 (MQ=255)                                            gaCAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCa                                                                                        <  1:265464/139‑1 (MQ=255)                                               aaTCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGtt                                                                                     <  2:71112/140‑1 (MQ=255)                                                    cgcgTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCaa                                                                                >  1:222054/1‑140 (MQ=255)                                                     gcgTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGgcg                                                                                          <  1:39066/129‑1 (MQ=255)                                                     gcgTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGgcg                                                                                          >  2:39066/1‑129 (MQ=255)                                                           acacTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACg                                                                         <  1:661268/140‑1 (MQ=255)                                                                tATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGc                                                                    <  1:211156/140‑1 (MQ=255)                                                                   tCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTAc                                                                 >  1:435596/1‑140 (MQ=255)                                                                                         acacGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATaaa                                           >  2:455624/1‑140 (MQ=255)                                                                                               aCATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGAcc                                     <  1:396535/140‑1 (MQ=255)                                                                                                   ggCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGAt                                              <  2:684179/127‑1 (MQ=255)                                                                                                   ggCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGAt                                              >  1:684179/1‑127 (MQ=255)                                                                                                   ggCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGc                                  <  2:12741/139‑1 (MQ=255)                                                                                                   ggCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGc                                  >  1:12741/1‑139 (MQ=255)                                                                                                   ggCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCg                                 >  2:357175/1‑140 (MQ=255)                                                                                                    gCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCgg                                   <  1:222810/137‑1 (MQ=255)                                                                                                    gCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCgg                                   >  2:222810/1‑137 (MQ=255)                                                                                                    gCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCga                                <  2:216809/140‑1 (MQ=255)                                                                                                    gCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCga                                <  2:235215/140‑1 (MQ=255)                                                                                                         cagAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGa                           >  1:527894/1‑140 (MQ=255)                                                                                                         cagAGGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGa                           >  1:354433/1‑140 (MQ=255)                                                                                                            agGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAg                        >  1:326908/1‑140 (MQ=255)                                                                                                            agGCGTAAACAAGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAg                        <  1:467828/140‑1 (MQ=255)                                                                                                                       aGGTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa             <  1:357175/140‑1 (MQ=255)                                                                                                                        ggTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAAt            >  2:142807/1‑140 (MQ=255)                                                                                                                        ggTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAAt            >  2:120803/1‑140 (MQ=255)                                                                                                                        ggTTATTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAAt            >  1:796019/1‑140 (MQ=255)                                                                                                                            aTTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCa        <  2:527894/140‑1 (MQ=255)                                                                                                                            aTTCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCa        <  2:354433/140‑1 (MQ=255)                                                                                                                             ttCTCGTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAc       >  2:629163/1‑140 (MQ=255)                                                                                                                                  gTTGGTAGTCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGt  >  1:510013/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGT  >  NC_000913/4274029‑4274297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑