Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,310,086 C→T G299S (GGT→AGT)  alsA ← D‑allose ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,310,0860CT96.4% 181.2 / ‑1.8 55G299S (GGT→AGT) alsAD‑allose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (24/29);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCC  >  NC_000913/4309956‑4310219
                                                                                                                                  |                                                                                                                                     
tAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTcc                                                                                                                              >  2:575892/1‑140 (MQ=255)
tAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc                                                                                                                              >  2:742163/1‑140 (MQ=255)
   gCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGc                                                                                                                           >  2:655983/1‑140 (MQ=255)
    ccATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCa                                                                                                                          <  2:635782/140‑1 (MQ=255)
    ccATCCCTTTATTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCa                                                                                                                          <  1:39123/140‑1 (MQ=255)
       tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAg                                                                                                                       >  2:404700/1‑140 (MQ=255)
       tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAg                                                                                                                       >  2:589750/1‑140 (MQ=255)
        cccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACGATTCATCAGTTCAGTACGTCCGGAACTGAc                                                                                                                                    >  1:63060/1‑126 (MQ=255)
        cccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACGATTCATCAGTTCAGTACGTCCGGAACTGAc                                                                                                                                    <  2:63060/126‑1 (MQ=255)
        cccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGAc                                                                                                                                    >  1:170439/1‑126 (MQ=255)
        cccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGAc                                                                                                                                    <  2:170439/126‑1 (MQ=255)
        cccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAg                                                                                                                       >  2:796050/1‑139 (MQ=255)
        cccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAg                                                                                                                       <  1:796050/139‑1 (MQ=255)
           ttttttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTa                                                                                                                   <  1:366336/140‑1 (MQ=255)
              tttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCa                                                                                                                          >  2:523727/1‑130 (MQ=255)
              tttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCa                                                                                                                          <  1:523727/130‑1 (MQ=255)
                       aTCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTtctc                                                                                                          >  1:20146/1‑137 (MQ=255)
                       aTCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTtctc                                                                                                          <  2:20146/137‑1 (MQ=255)
                         ccAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCgg                                                                                                     <  1:575892/140‑1 (MQ=255)
                            ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAg                                                                                                  <  1:439436/140‑1 (MQ=255)
                               gTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACg                                                                                               <  2:656795/140‑1 (MQ=255)
                                 gAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCt                                                                                             <  1:589750/140‑1 (MQ=255)
                                 gAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCt                                                                                             <  1:655983/140‑1 (MQ=255)
                                  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCa                                                                                                   >  1:378172/1‑133 (MQ=255)
                                  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCa                                                                                                   <  2:378172/133‑1 (MQ=255)
                                  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCa                                                                                                   <  2:448025/133‑1 (MQ=255)
                                  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCa                                                                                                   >  1:448025/1‑133 (MQ=255)
                                  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTa                                                                                            >  1:313545/1‑140 (MQ=255)
                                  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTa                                                                                            >  2:227620/1‑140 (MQ=255)
                                  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTa                                                                                            >  1:456409/1‑140 (MQ=255)
                                   aCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTaa                                                                                           <  1:742163/140‑1 (MQ=255)
                                    cGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTaaa                                                                                          <  1:774137/140‑1 (MQ=255)
                                    cGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTaaa                                                                                          <  1:141961/140‑1 (MQ=255)
                                                tCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCg                                                                              <  1:227620/140‑1 (MQ=255)
                                                       cATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCttt                                                                       <  1:404700/140‑1 (MQ=255)
                                                         ttAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCttttt                                                                     <  2:528861/140‑1 (MQ=255)
                                                           aaGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTc                                                                   >  2:412682/1‑140 (MQ=255)
                                                                       tcCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTgg                                                       <  2:698899/140‑1 (MQ=255)
                                                                        ccGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGt                                                      <  1:412682/140‑1 (MQ=255)
                                                                         cGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTg                                                     <  1:557902/140‑1 (MQ=255)
                                                                               cgcgTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTg                                                     <  1:258966/134‑1 (MQ=255)
                                                                               cgcgTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTg                                                     >  2:258966/1‑134 (MQ=255)
                                                                                  gTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGc                                            <  1:648403/140‑1 (MQ=255)
                                                                                              cAAACAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAAcc                                <  1:762896/140‑1 (MQ=255)
                                                                                                aaCAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGt                              >  1:298155/1‑140 (MQ=255)
                                                                                                 aCAGACAATTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGtt                             >  1:311329/1‑140 (MQ=255)
                                                                                                       aaTTCATCAGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTgc                       >  2:140849/1‑140 (MQ=255)
                                                                                                            atcaGTTCAGTACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAg                  >  2:539206/1‑140 (MQ=255)
                                                                                                                gttcagtACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCGCGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTg              <  1:268955/140‑1 (MQ=255)
                                                                                                                      tACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACa        <  2:456409/140‑1 (MQ=255)
                                                                                                                      tACGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACCTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACa        >  1:514013/1‑140 (MQ=255)
                                                                                                                        cGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGt                >  2:608461/1‑130 (MQ=255)
                                                                                                                        cGTCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGt                <  1:608461/130‑1 (MQ=255)
                                                                                                                          tCCGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATtct    >  1:168955/1‑140 (MQ=255)
                                                                                                                            cGGAACTGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTcc  >  2:775047/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                     
TAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCC  >  NC_000913/4309956‑4310219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑